About: Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4

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About: Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4
Creator	Osterhaus, Albert Baumgärtner, Wolfgang Lehmbecker, Annika Segalés, Joaquim Bensaid, Albert Haagmans, Bart Haverkamp, Ann-Kathrin Spitzbarth, Ingo Vergara-Alert, Julia Widagdo, Widagdo Van Den Brand, Judith
Source	Medline; PMC
abstract	Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one-third of human patients. Recent data indicate that dromedaries represent an important source of infection, although information regarding viral cell tropism and pathogenesis is sparse. In the current study, tissues of eight dromedaries receiving inoculation of MERS-Coronavirus (MERS-CoV) after recombinant Modified-Vaccinia-Virus-Ankara (MVA-S)-vaccination (n = 4), MVA-vaccination (mock vaccination, n = 2) and PBS application (mock vaccination, n = 2), respectively, were investigated. Tissues were analyzed by histology, immunohistochemistry, immunofluorescence, and scanning electron microscopy. MERS-CoV infection in mock-vaccinated dromedaries revealed high numbers of MERS-CoV-nucleocapsid positive cells, T cells, and macrophages within nasal turbinates and trachea at day four post infection. Double immunolabeling demonstrated cytokeratin (CK) 18 expressing epithelial cells to be the prevailing target cell of MERS-CoV, while CK5/6 and CK14 expressing cells did not co-localize with virus. In addition, virus was occasionally detected in macrophages. The acute disease was further accompanied by ciliary loss along with a lack of dipeptidyl peptidase 4 (DPP4), known to mediate virus entry. DPP4 was mainly expressed by human lymphocytes and dromedary monocytes, but overall the expression level was lower in dromedaries. The present study underlines significant species-specific manifestations of MERS and highlights ciliary loss as an important finding in dromedaries. The obtained results promote a better understanding of coronavirus infections, which pose major health challenges.
has issue date	2018-06-27(xsd:dateTime)
bibo:doi	10.1038/s41598-018-28109-2
bibo:pmid	29950581
has license	cc-by
sha1sum (hex)	01234cee305e37a78cedea8148bf6177d47fe66b
schema:url	https://doi.org/10.1038/s41598-018-28109-2
resource representing a document's title	Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4
has PubMed Central identifier	PMC6021449
has PubMed identifier	29950581
schema:publication	Sci Rep
resource representing a document's body	covid:01234cee305e37a78cedea8148bf6177d47fe66b#body_text
is schema:about of	named entity 'current' named entity 'infection' named entity 'cell surface receptor' covid:arg/01234cee305e37a78cedea8148bf6177d47fe66b named entity 'Recent' named entity 'MERS' named entity 'IL-6' named entity 'CD20' named entity 'nasal mucosa' named entity 'antigens' named entity 'DPP4' named entity 'α-tubulin' named entity 'CD20' named entity 'trachea' named entity 'MERS-CoV' named entity 'immunohistochemistry' named entity 'inflammatory cells' named entity 'pathogenesis' named entity 'peptidase' named entity 'dromedaries' named entity 'Tissues' named entity 'respiratory disease' named entity 'MERS-CoV' named entity 'infection' named entity 'cell surface receptor' named entity 'infection' named entity 'dromedaries' named entity 'Immunoglobulin' named entity 'submucosa' named entity 'PBMC' named entity 'MERS-CoV' named entity 'goat' named entity 'lymphocytes' named entity 'nucleocapsid' named entity 'monoclonal antibodies' named entity 'antibody' named entity 'DPP4' named entity 'DPP4' named entity 'immunofluorescence' named entity 'MERS-CoV' named entity 'macrophages' named entity 'dromedaries' named entity 'MERS-CoV' named entity 'Netherlands' named entity 'epithelial' named entity 'flow cytometry' named entity 'coronavirus infection' named entity 'lymph nodes' named entity 'lamina propria' named entity 'MERS-CoV' named entity 'CD3' named entity 'Tissues' named entity 'abomasum' named entity 'epithelium' named entity 'DPP4' named entity 'antigen' named entity 'serological surveys' named entity 'epithelial cells' named entity 'enterocytes' named entity 'ascites fluid' named entity 'viral particles' named entity 'MERS-CoV' named entity 'nucleocapsid' named entity 'monocytes' named entity 'antibodies' named entity 'lymph nodes' named entity 'live/dead' named entity 'domestic livestock' named entity 'epithelial cells' named entity 'MERS-CoV' named entity 'camel'

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