About: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin

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About: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
Creator	Martin, F Thomas, Monitoring
Source	PMC
abstract	The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disrupts Golgi stack formation and impairs critical functions of the Golgi, such as accurate protein glycosylation and sorting. However, how Golgi destruction affects other cellular activities is so far unknown. Here, we report that depletion of GRASP proteins reduces cell attachment and migration. Interestingly, GRASP depletion reduces the protein level of α5β1 integrin, the major cell adhesion molecule at the surface of HeLa and MDA-MB-231 cells, due to decreased integrin protein synthesis. GRASP depletion also increases cell growth and total protein synthesis. These new findings enrich our understanding on the role of the Golgi in cell physiology and provide a potential target for treating protein-trafficking disorders.
has issue date	2019-03-15(xsd:dateTime)
bibo:doi	10.1091/mbc.e18-07-0462
bibo:pmid	30649990
has license	cc-by-nc-sa
sha1sum (hex)	02450c2c82a1511cb5e1639d3b8438f69bbe11f0
schema:url	https://doi.org/10.1091/mbc.e18-07-0462
resource representing a document's title	GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of α5β1 integrin
has PubMed Central identifier	PMC6589770
has PubMed identifier	30649990
schema:publication	Mol Biol Cell
resource representing a document's body	covid:02450c2c82a1511cb5e1639d3b8438f69bbe11f0#body_text
is schema:about of	covid:arg/02450c2c82a1511cb5e1639d3b8438f69bbe11f0 named entity '766' named entity 'posttranslational modification' named entity 'trypsinized' named entity 'marker proteins' named entity 'human plasma' named entity 'GRASP55' named entity 'Golgi' named entity 'integrin' named entity 'extracellular space' named entity 'GRASP55' named entity 'GFP' named entity 'GRASP55' named entity 'homeostasis' named entity 'exogenously' named entity 'MgCl' named entity 'α5β1 integrin' named entity 'wild-type' named entity '2015, 2016' named entity 'Golgi' named entity 'Golgi' named entity 'Western blotting' named entity 'integrin' named entity 'Integrins' named entity 'integrin' named entity 'Golgi' named entity 'eluted' named entity 'integrin' named entity 'cell growth' named entity 'wound-healing' named entity 'protein' named entity 'kDa' named entity 'serum' named entity 'β1 integrins' named entity 'Germany' named entity 'protein' named entity 'plasma membrane' named entity 'integrin' named entity 'vesicles' named entity 'integrin' named entity 'proteolysis' named entity 'room temperature' named entity 'glycosylation' named entity 'Western blot' named entity 'GRASP55' named entity 'GE Healthcare' named entity 'methanol' named entity 'integrin' named entity 'integrin' named entity 'transfection' named entity 'radioactive labeling' named entity 'GRASP65' named entity 'cisternae' named entity 'cell lysate' named entity 'field of view' named entity 'GFP' named entity 'siRNA' named entity 'GRASP65' named entity 'α5β1' named entity 'crystal violet' named entity 'siRNA' named entity 'Golgi' named entity 'HeLa' named entity 'HeLa' named entity 'α5β1 integrin' named entity 'cis-Golgi' named entity 'sodium fluoride' named entity 'β1-integrin' named entity 'growth medium' named entity 'protein' named entity 'Student's t test' named entity 'autoradiography' named entity 'SDS' named entity 'pipette' named entity 'DTT' named entity 'GRASP65' named entity 'Golgi' named entity 'protease inhibitor' named entity 'phosphorylation' named entity 'transfection'

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