About: RNA binding properties of core protein of the flavivirus Kunjin

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About: RNA binding properties of core protein of the flavivirus Kunjin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	RNA binding properties of core protein of the flavivirus Kunjin
Creator	Khromykh, A Westaway, E
source	Medline; PMC
abstract	Kunjin virus (KUN) C is a typical flavivirus core protein which is truncated in vivo to a mature form of 105 residues enriched in lysine and arginine. In order to study the possible association of KUN C with RNA in vitro, we prepared several recombinant C proteins with specific deletions, each fused at the amino-terminus to glutathione-S-transferase (GST) and expressed inE. coli. They were reacted with KUN RNA probes transcribed in vitro from cDNA representing the 5′ untranslated region (5′ UTR, 93 of 96 nucleotides), the 3′ UTR (624 nucleotides), and the 5′ UTR plus most of the C coding region (t′ core, 440 nucleotides). Fusion protein C107 (incorporating mature C) bound strongly to all KUN RNA probes with apparent specificity, being completely resistant to inhibition by 800 mM NaCl, and to competition by a large excess of tRNA. In reactions with labelled KUN RNA probes putative binding sites were identified in the isolated amino-terminal (32 residues) and carboxyterminal (26 residues) basic amino acid domains; this binding was strongly competed by unlabelled KUN UTR probes but weakly or not at all by tRNA. These small domains probably acted co-operatively in binding of mature C to KUN RNA probes. The KUN RNA-core protein binding reactions are similar to those reported with other viral coat or capsid proteins and viral RNAs.
has issue date	1996-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01718326
bibo:pmid	8645104
has license	no-cc
sha1sum (hex)	029d03ec8aef8ce9bba1d88f71f49471e784038b
schema:url	https://doi.org/10.1007/bf01718326
resource representing a document's title	RNA binding properties of core protein of the flavivirus Kunjin
has PubMed Central identifier	PMC7086803
has PubMed identifier	8645104
schema:publication	Arch Virol
resource representing a document's body	covid:029d03ec8aef8ce9bba1d88f71f49471e784038b#body_text
is schema:about of	named entity 'expressed' named entity 'RNA' named entity 'co-operatively' named entity 'proteins' named entity 'glutathione-S-transferase' named entity 'flavivirus' named entity 'PROTEIN ' named entity 'IN VIVO' named entity 'UNTRANSLATED REGION' named entity 'ENRICHED' named entity 'EXPRESSED' named entity 'BASIC AMINO ACID' named entity 'PREPARED' named entity 'E. COLI' named entity 'RNA PROBES' named entity 'AMINO-TERMINUS' named entity 'CAPSID PROTEINS' named entity 'TRANSFERASE' named entity 'ORDER' named entity 'INHIBITION' named entity 'PROTEIN BINDING' named entity 'LYSINE' named entity 'RECOMBINANT' named entity 'IN VITRO' named entity 'BUT' named entity 'POSSIBLE' named entity 'C PROTEINS' named entity 'COMPETITION' named entity 'VIRAL' named entity 'FORM' named entity 'TRANSCRIBED' named entity 'NUCLEOTIDES' named entity 'FUSED' named entity 'CORE PROTEIN' named entity 'PROBES' named entity '28624' named entity 'SPECIFIC' named entity 'TYPICAL' named entity 'REACTIONS' named entity 'COAT' named entity 'LARGE' named entity 'CODING REGION' named entity 'THESE' named entity 'PROPERTIES' named entity 'SMALL' named entity 'BINDING SITES' named entity 'APPARENT' named entity 'TRNA' named entity 'FLAVIVIRUS' named entity 'BINDING' named entity 'EXCESS' named entity 'RNA' named entity 'UTR' named entity 'MOST OF' named entity 'TRUNCATED' named entity 'NAC1' named entity 'IS A' named entity 'IDENTIFIED' named entity '800' named entity 'FLAVIVIRUS' named entity 'RNA BINDING' named entity 'AMINO' named entity 'ASSOCIATION' named entity 'RESISTANT TO' named entity 'BEING' named entity 'NOT AT ALL' named entity 'SPECIFICITY' named entity 'MATURE' named entity 'CORE' named entity 'TERMINAL' named entity 'PROBABLY' named entity 'STUDY' named entity 'CDNA' named entity 'FUSION PROTEIN' named entity 'KUNJIN VIRUS' named entity 'COMPLETELY' named entity 'PROTEIN ' named entity 'SIMILAR' named entity 'GLUTATHIONE' named entity 'CORE PROTEIN'

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