About: Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples

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About: Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples
Creator	Yang, Bo Shen, Guanxin Gan, Yan Huang, Chunmei Lei, Ping Liu, Hongbo Weng, Hui Yang, Daofeng
Source	Medline; PMC
abstract	This study evaluated the performance of the Maxwell 16 System (Promega) for extraction of influenza virus (flu-v) RNA from diverse samples compared to a classical manual method (QIAamp Kit, QIAGEN). Following extraction by the two methods, all samples were analyzed by Real-time RT-PCR. Results revealed that the use of the standard Maxwell 16 protocol (Maxwell 16-S) resulted in good linearity and precision across a wide concentration range and higher sensitivity of detection from flu-v stock suspensions than the manual method. Compared with the latter method, Maxwell 16-S extracted RNA more efficiently (higher RNA yield and/or fewer PCR inhibitors) from throat swabs and bronchoalveolar lavage fluids, while both methods performed comparably on fecal samples from human and poultry in terms of overall threshold cycle values and detection rates although the Maxwell 16-S co-purified more inhibitors from fecal samples. The capacity of this system to remove inhibitors from fecal matrix was improved by using a modified Maxwell 16 protocol with a reduced sample input, which eliminated all false-negatives produced by the Maxwell 16-S. These findings suggest that the Maxwell 16 System is suitable for RNA extraction from multiple-source samples for diagnosis of influenza and viral load determination and that a proper reduction in starting sample volume may improve the detection of flu-v from complex matrices such as feces. Additionally, this system allows flexible sample throughput and labor-saving sample processing with little or no risk of cross-contamination.
has issue date	2012-10-29(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0048094
bibo:pmid	23144730
has license	cc-by
sha1sum (hex)	02a9fd5f564cb3a5b6b1fdbcd9df585b8e24a2de
schema:url	https://doi.org/10.1371/journal.pone.0048094
resource representing a document's title	Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples
has PubMed Central identifier	PMC3483271
has PubMed identifier	23144730
schema:publication	PLoS One
resource representing a document's body	covid:02a9fd5f564cb3a5b6b1fdbcd9df585b8e24a2de#body_text
is schema:about of	named entity 'standard' named entity 'study' named entity 'starting' named entity 'eliminated' named entity 'Kit' named entity 'Promega' named entity 'System' covid:arg/02a9fd5f564cb3a5b6b1fdbcd9df585b8e24a2de named entity 'sample' named entity 'suspensions' named entity 'sample' named entity 'virus' named entity 'samples' named entity 'Maxwell' named entity 'detection' named entity 'QIAGEN' named entity 'PCR' named entity 'viral load' named entity 'performance' named entity 'reduction' named entity 'wide' named entity 'protocol' named entity 'extracted' named entity 'Maxwell' named entity 'values' named entity 'flu' named entity 'RNA' named entity 'viral load' named entity 'RT-PCR' named entity 'Influenza Virus' named entity 'regression analysis' named entity 'PCR' named entity 'PCR' named entity 'sample matrix' named entity 'feces' named entity 'Viral' named entity 'PCR' named entity 'virus' named entity 'Flu' named entity 'viral RNA' named entity 'human plasma' named entity 'false-positive' named entity 'W/V' named entity 'CDC' named entity 'PCR-based' named entity 'phlegm' named entity 'Wilcoxon signed rank test' named entity 'QIAGEN' named entity 'seasonal flu' named entity 'reagent' named entity 'feces' named entity 'SPSS' named entity 'freeze-thaw' named entity 'sample matrix' named entity 'viruses' named entity '13.0' named entity 'BALF' named entity 'fluorescent reporter' named entity 'Promega' named entity 'PCR' named entity 'flu' named entity 'PCR' named entity 'pH1N1' named entity 'highly pathogenic avian influenza' named entity '2009 pandemic' named entity 'coextracted' named entity 'United States' named entity 'cross-contamination' named entity 'cytomegalovirus' named entity 'reagent' named entity 'PCR amplification' named entity 'PCR' named entity 'McNemar test' named entity 'RNA extraction'

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