About: Direct binding of autoimmune disease related T cell epitopes to purified Lewis rat MHC class II molecules

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About: Direct binding of autoimmune disease related T cell epitopes to purified Lewis rat MHC class II molecules Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Direct binding of autoimmune disease related T cell epitopes to purified Lewis rat MHC class II molecules
Creator	Pedersen, Lars Hensen, Evert Van Eden, Willem Wauben, Marca Joosten, Irma Buus, S^ren Holewijn, Monlek Reske, Konrad Roosenboom, Cyril
Source	PMC
abstract	New strategies applied in the treatment of experimental autoimmune disease models involve blocking or modulation of MHC–peptide–TCR interactions either at the level of peptide–MHC interaction or, alternatively, at the level of T cell recognition. In order to identify useful competitor peptldes one must be able to assess peptide–MHC interactions. Several well described autoimmune disease models exist in the Lewis rat and thus this particular rat strain provides a good model system to study the effect of competitor peptldes. So far no information has been available on the peptide binding characteristics of the Lewis rat MHC class II RT1.B(I) molecule. We have now developed a biochemical binding assay which enables competition studies in which the relative MHC binding affinity of a set of non-labelled peptldes can be assessed while employing detection of blotlnylated marker peptides by chemllumlnescence. The assay is sensitive and specific. We have used this assay to determine the binding characteristics of several disease associated T cell determinants and their sequence analogues in the Lewis rat. Notably, most of the autoimmune disease associated peptide sequences tested were found to be intermediate to poor binders. Single amino acid substitutions at defined positions were sufficient to turn certain peptldes into good binders. These results are relevant to the design of competitor peptldes in the treatment of experimental autoimmune diseases.
has issue date	1994-05-01(xsd:dateTime)
bibo:doi	10.1093/intimm/6.5.751
bibo:pmid	7521668
has license	no-cc
sha1sum (hex)	0368cf718d76eea0e4ae99786b9459074038db7e
schema:url	https://doi.org/10.1093/intimm/6.5.751
resource representing a document's title	Direct binding of autoimmune disease related T cell epitopes to purified Lewis rat MHC class II molecules
has PubMed Central identifier	PMC7110089
has PubMed identifier	7521668
schema:publication	Int Immunol
resource representing a document's body	covid:0368cf718d76eea0e4ae99786b9459074038db7e#body_text
is schema:about of	named entity 'peptide' named entity 'strategies' named entity 'strain' named entity 'bear' named entity 'epitopes' named entity 'autoimmune disease' named entity 'DISEASE ASSOCIATED' named entity 'INTERFERENCE' named entity 'CHARACTERISTICS' named entity 'STRUCTURAL' named entity 'DETERMINE' named entity 'LEVEL' named entity 'EXPERIMENTAL' named entity 'BUT' named entity 'THESE' named entity 'NEW' named entity 'STIMULATING' named entity 'MOLECULE' named entity 'THEIR' named entity 'DESIGN' named entity 'POSITIONS' named entity 'EXPECTED' covid:arg/0368cf718d76eea0e4ae99786b9459074038db7e named entity 'structural' named entity 'alternatively' named entity 'binders' named entity 'order' named entity 'Into' named entity 'close' named entity 'expected' named entity 'sequences' named entity 'binding' named entity 'peptides' named entity 'binding affinity' named entity 'autoimmune diseases' named entity 'assay' named entity 'RT1' named entity 'MHC' named entity 'amino acid' named entity 'peptide' named entity 'Lewis rat' named entity 'Lewis rat' named entity 'MHC molecule' named entity 'disease' named entity 'clone' named entity 'immunological' named entity 'MHC' named entity 'rat' named entity 'rat' named entity 'biotinylated' named entity 'MHC' named entity 'dialysis' named entity 'MHC' named entity 'biochemical' named entity 'biotinylation' named entity 'thymocytes' named entity 'autoradiographs' named entity 'IC50' named entity 'relative binding affinity' named entity 'peptide' named entity 'autoimmune disease' named entity 'OVA peptide' named entity 'rodent' named entity 'peptides' named entity 'protease inhibitor' named entity 'peptides' named entity 'footpad' named entity 'allele' named entity 'uveoretinitis' named entity 'peptides' named entity 'Peptide binding' named entity 'peptide' named entity 'MHC'

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