About: A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2

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About: A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2
Creator	Reynolds, S Bisha, B Chandler, J Davidson, M Franklin, A Goodridge, L Magzamen, S Pérez-Méndez, A Schaeffer, J Shriner, S Volckens, J
source	Elsevier; Medline; PMC
abstract	Abstract The detection of aerosolized viruses can serve as an important surveillance and control tool in agriculture, human health, and environmental settings. Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. In this method, aerosolized viruses are collected in a 20ml liquid sample contained within widely used impingers, BioSamplers (SKC Inc., Eighty Four, PA), and further concentrated via adsorption to an anion exchange resin that is suspended within this liquid. Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. For this study, various quantities of two negatively charged viruses, type A and type B influenza viruses (FluMist Quadrivalent vaccine) and the male-specific (F+) RNA coliphage MS2 (MS2), were nebulized into a custom-built bioaerosolization chamber, and sampled using BioSamplers with and without anion exchange resin. Compared to direct testing of the BioSampler liquid, detection was improved by 6.77× and 3.33× for type A and type B influenza viruses, respectively, by using the anion exchange resin. For MS2, the anion exchange resin method allowed for an average improvement in detection of 8.26×.
has issue date	2017-08-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2017.04.004
bibo:pmid	28450173
has license	els-covid
sha1sum (hex)	03d37bd0d8f532abc81284a3bdd38afc3e376873
schema:url	https://doi.org/10.1016/j.jviromet.2017.04.004
resource representing a document's title	A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2
has PubMed Central identifier	PMC7113747
has PubMed identifier	28450173
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:03d37bd0d8f532abc81284a3bdd38afc3e376873#body_text
is schema:about of	named entity 'Bisha' named entity 'influenza viruses' named entity 'Quadrivalent vaccine' named entity 'anion exchange resin' named entity 'inocula' named entity 'enteric viruses' named entity 'anion exchange resin' named entity 'anion exchange resin' named entity 'water samples' named entity 'anion exchange resin' named entity 'resin-based' named entity 'FFU' named entity 'bioaerosols' named entity 'live attenuated' named entity 'U.S.' named entity 'nebulizer' named entity 'Nebulization' named entity 'anion exchange resin' named entity 'anion exchange resin' named entity 'qRT-PCR' named entity 'Brisbane' named entity 'titer' named entity 'aerosol' named entity 'bioaerosol' named entity 'nebulization' named entity 'rotavirus' named entity 'nebulization' named entity 'bioaerosols' named entity 'nebulization' named entity 'viruses' named entity 'vaccine' named entity 'FluMist Quadrivalent' named entity 'environmental conditions' named entity 'influenza viruses' named entity 'inocula' named entity 'qRT-PCR' named entity 'water sample' named entity 'reverse transcription PCR' named entity 'qPCR' named entity 'axial fans' named entity 'carbon dioxide' named entity 'resin' named entity 'enteric viruses' named entity 'viruses' named entity 'HEPA' named entity 'aerosols' named entity 'aerosolization' named entity 'relative mass' named entity 'bioaerosol' named entity 'adsorb' named entity 'nebulizer' named entity 'quantitative real-time PCR' named entity 'influenza' named entity 'FluMist' named entity 'nebulizer' named entity 'qRT-PCR' named entity 'inocula' named entity 'influenza viruses' named entity 'viral transmission' named entity 'nucleic acids' named entity 'qRT-PCR' named entity 'aqueous' named entity 'physicochemical properties' named entity 'bioaerosol' named entity 'ppm' named entity 'PBS' named entity 'inoculum' named entity '3.5' named entity 'dechlorinated' named entity 'bioaerosol' named entity 'viruses'

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