About: Construction of Armored RNA Containing Long-Size Chimeric RNA by Increasing the Number and Affinity of the Pac Site in Exogenous RNA and Sequence Coding Coat Protein of the MS2 Bacteriophage

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About: Construction of Armored RNA Containing Long-Size Chimeric RNA by Increasing the Number and Affinity of the Pac Site in Exogenous RNA and Sequence Coding Coat Protein of the MS2 Bacteriophage Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Construction of Armored RNA Containing Long-Size Chimeric RNA by Increasing the Number and Affinity of the Pac Site in Exogenous RNA and Sequence Coding Coat Protein of the MS2 Bacteriophage
Creator	Wang, Wei Liu, Min Wang, Jing Zhang, Rui Li, Jinming Zhang, Kuo Wang, Lunan Wei, Baojun Wei, Yuxiang Yang, Changmei Lin, Guigao Xu, Ruihuan Zhan, Sien
source	Medline; PMC
abstract	OBJECTIVES: To construct a one-plasmid expression system of the armored RNA containing long chimeric RNA byincreasing the number and affinity of the pac site. METHODS: The plasmid pET-MS2-pac was constructedwith one C-variant pac site, and then the plasmid pM-CR-2C containing 1,891-bp chimeric sequences and two C-variant pac sites was produced. Meanwhile, three plasmids (pM-CR-C, pM-CR-2W and pM-CR-W) were obtained as parallel controls with a different number and affinity of the pac site. Finally, the armored RNA was expressed and purified. RESULTS: The armored RNA with 1,891 bases target RNA was expressed successfully by the one-plasmid expression system with two C-variant pac sites, while for one pac site, no matter whether the affinity was changed or not, only the 1,200 bases target RNA was packaged. It was also found that the C-variant pac site could increase the expression efficiency of the armored RNA. The armored RNA with 1,891-bp exogenous RNA in our study showed the characterization of ribonuclease resistance and stability at different time points and temperature conditions. CONCLUSIONS: The armored RNA with 1,891 bases exogenous RNA was constructed and the expression system can be used as a platform for preparation of the armored RNA containing long RNA sequences.
has issue date	2008-07-01(xsd:dateTime)
bibo:doi	10.1159/000141707
bibo:pmid	18594159
has license	no-cc
sha1sum (hex)	040014a46da8e6af9ebee173c762de46b180fca9
schema:url	https://doi.org/10.1159/000141707
resource representing a document's title	Construction of Armored RNA Containing Long-Size Chimeric RNA by Increasing the Number and Affinity of the Pac Site in Exogenous RNA and Sequence Coding Coat Protein of the MS2 Bacteriophage
has PubMed Central identifier	PMC7179527
has PubMed identifier	18594159
schema:publication	Intervirology
resource representing a document's body	covid:040014a46da8e6af9ebee173c762de46b180fca9#body_text
is schema:about of	named entity 'RNA' named entity 'temperature' named entity 'armored' named entity 'RNA' named entity 'RNA' named entity 'RNA' named entity 'SARS2' named entity 'RNA' named entity 'RT-PCR' named entity 'RNA' named entity 'nucleotide' named entity 'SARS' named entity 'RNA' named entity 'exogenous' named entity 'sonication' named entity 'plasmid' named entity 'E. coli' named entity 'RNA' named entity 'plasmid' named entity 'RNA' named entity 'RNA' named entity 'SARS' named entity 'plasmid' named entity 'GenBank' named entity 'RNA' named entity 'MS2' named entity 'GenBank' named entity 'RNA' named entity 'RNA' named entity 'RT-PCR' named entity 'maturase' named entity 'wild-type' named entity 'stem-loop region' named entity 'plasmid' named entity 'pH 8' named entity 'Promega' named entity 'MS2' named entity 'RNA sequences' named entity 'RNA' named entity 'RNA' named entity 'plasmid' named entity 'ligated' named entity 'plasmid' named entity 'RNA' named entity 'gradient centrifugation' named entity 'RNA' named entity 'coat protein' named entity 'RNA' named entity 'RNA' named entity 'RNA' named entity 'plasmid' named entity 'clinical laboratories' named entity 'viral genomes' named entity 'chimeric' named entity 'chimeric' named entity 'RNA' named entity 'SARS' named entity 'chimeric' named entity 'Novagen' named entity 'RT-PCR' named entity 'RNA' named entity 'GenBank' named entity 'PCR' named entity 'HCV' named entity 'coat protein' named entity 'RNA' named entity 'Germany' named entity 'HCV' named entity 'recombinant'

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