About: Detección automática de bacterias y hongos en sangre

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detección automática de bacterias y hongos en sangre
Creator	Córdoba, Juan Gobernado, Miguel Ramírez, Paula Molina, José
Source	Elsevier; PMC
abstract	La sepsis es una de las principales causas de mortalidad y morbilidad en los hospitales. La detección temprana de los patógenos mediante técnicas basadas en ácidos nucleicos puede facilitar el diagnóstico rápido de la bacteriemia y/o fungemia, la rápida adecuación del tratamiento antibiótico, reducir el uso de antibióticos innecesarios y disminuir la mortalidad. Se describen 2 técnicas disponibles comercialmente que ayudan, en un tiempo reducido, a identificar distintas bacterias y hongos productores de sepsis: LightCycler® SeptiFast Test Mgrade (Roche Diagnostic SL) y GenoType Blood Culture (Hain Lifescience). De estas 2 técnicas, mostramos los resultados de un estudio inicial propio con el LightCycler® SeptiFast Test Mgrade. El estudio se realizó con 50 muestras correspondientes a 28 pacientes (1-3 muestras por paciente) con síndrome séptico ingresados en la unidad de cuidados intensivos, comparando la nueva técnica con el hemocultivo convencional. La concordancia entre los resultados del hemocultivo y el SeptiFast fue buena, 79% en el primer ensayo, y 89% en el segundo, después de corregir defectos técnicos. Inicialmente se observó inhibición importante de los controles internos para los bacilos gramnegativos, por la presencia de heparina en la sangre empleada y un exceso de ácido desoxirribonucleico (ADN) debido al número de leucocitos. Con la finalidad de disminuir las inhibiciones, en otro estudio posterior se trabajó con 24 muestras a la mitad de volumen original, llevando el ácido nucleico extraído a dilución 1:4. Con estas modificaciones, se apreciaron reducciones importantes de las inhibiciones. En comparación con el hemocultivo, el SeptiFast diferencia mejor las contaminaciones por estafilococos coagulasa-negativa y especies de estreptococos. Sepsis is one of the main causes of mortality and morbidity in hospitals. Early detection of pathogens using nucleic acid-based techniques speeds diagnosis of bacteremia and/or fungemia, aids the rapid application of appropriate antibiotics, reduces the use of unnecessary antibiotics, and lowers mortality. Two commercially available techniques that help to identify different sepsisproducing bacteria and fungi in a shorter time period are: LightCycler® SeptiFast Test Mgrade (Roche Diagnostic SL) and GenoType Blood Culture (Hain Lifescience). We present the results of an initial in-house study using the LightCycler® SeptiFast Test Mgrade. The study was carried out in 50 samples from 28 patients (1-3 samples per patient) with septic syndrome admitted to the intensive care unit by comparing the new technique with conventional blood culture. The concordance between the results of blood culture and SeptiFast was good, 79%, in the first trial and 89% in the second, after correcting for technical defects. We initially observed substantial inhibition of internal controls in Gram-negative bacilli, due to the presence of heparin in the blood used, and excess DNA because of the high number of leucocytes. To minimize these inhibitions, the second study used 24 samples at half the original volume (extracted DNA at 1/4 concentration). With these modifications, inhibitions were substantially reduced. SeptiFast is more effective than blood culture in discriminating between contamination by coagulase-negative staphylococci and species of streptococci.
has issue date	2008-07-31(xsd:dateTime)
bibo:doi	10.1016/s0213-005x(08)76544-3
bibo:pmid	19195450
has license	els-covid
sha1sum (hex)	04027550dc47568b1bdb2efae56610275028fcea
schema:url	https://doi.org/10.1016/s0213-005x%2808%2976544-3
resource representing a document's title	Detección automática de bacterias y hongos en sangre
has PubMed Central identifier	PMC7130238
has PubMed identifier	19195450
schema:publication	Enfermedades Infecciosas y Microbiología Clínica
resource representing a document's body	covid:04027550dc47568b1bdb2efae56610275028fcea#body_text
is schema:about of	named entity 'SANGRE' named entity 'AUTOMATIC' named entity 'inhibitions' named entity 'morbidity' named entity 'minimize' named entity 'excess' named entity 'intensive care unit' named entity 'inhibitions' named entity 'NUCLEIC ACID' named entity 'SEPTIC SYNDROME' named entity 'CAUSES' named entity 'USE OF' named entity 'RAPID' named entity 'SAMPLES' named entity 'GOOD' named entity 'VOLUME' named entity 'INHIBITIONS' named entity 'AIDS' named entity 'NEGATIVE' named entity 'DIAGNOSIS' named entity 'FUNGI ' named entity 'INTERNAL' named entity 'BACTEREMIA' named entity 'NEW' named entity 'DIFFERENT' named entity 'HOSPITALS' named entity 'SPEEDS' named entity 'FUNGEMIA' named entity 'INTENSIVE CARE UNIT' named entity 'CONCENTRATION' named entity 'PATIENT' named entity 'REDUCED' named entity 'APPLICATION' named entity 'INHIBITION' named entity 'ORIGINAL' named entity 'PATIENTS' named entity 'STUDY' named entity 'ONE OF' named entity 'BASED' named entity 'FUNGAL INFECTIONS' named entity 'IN BLOOD' named entity 'BLOOD CULTURE' named entity 'PRESENT' named entity 'ADMITTED TO' named entity 'SEPSIS' named entity 'MAIN' named entity 'SECOND' named entity 'THESE' named entity 'HELP' named entity 'EARLY DETECTION' named entity 'TEST' named entity 'EFFECTIVE' named entity 'USED' named entity 'IN-HOUSE' named entity 'INITIAL' named entity 'APPROPRIATE' named entity 'DIAGNOSTIC' named entity 'STREPTOCOCCI' named entity 'HEPARIN' named entity 'DETECTION' named entity 'BACILLI' named entity 'GRAM-' named entity 'BACTERIAL' named entity 'BACTERIAS' named entity 'SPECIES' named entity 'TRIAL' named entity 'BLOOD' named entity 'LEUCOCYTES' named entity 'BACTERIA ' named entity 'HALF' named entity 'OBSERVED' named entity 'LOWERS' named entity 'ANTIBIOTICS' named entity 'EXCESS' named entity 'HIGH' named entity 'CONCORDANCE' named entity 'TECHNIQUES' named entity 'TECHNIQUE'

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