About: Agreement Among 4 Sampling Methods to Identify Respiratory Pathogens in Dairy Calves with Acute Bovine Respiratory Disease

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Agreement Among 4 Sampling Methods to Identify Respiratory Pathogens in Dairy Calves with Acute Bovine Respiratory Disease
Creator	Aly, S Berghaus, L Berghaus, R Blanchard, P Champagne, J Cochran, S Credille, B Crossley, B Doyle, D Lehenbauer, T Woolums, A
Source	Medline; PMC
abstract	BACKGROUND: Four sampling techniques commonly are used for antemortem identification of pathogens from cattle with bovine respiratory disease (BRD): the nasal swab (NS), guarded nasopharyngeal swab (NPS), bronchoalveolar lavage (BAL), and transtracheal wash (TTW). Agreement among these methods has not been well characterized. OBJECTIVE: To evaluate agreement among TTW and NS, NPS, or BAL for identification of viral and bacterial pathogens in dairy calves with BRD. ANIMALS: One hundred dairy calves with naturally acquired BRD. METHODS: Calves were sampled by all 4 methods. Viral agents were identified by real‐time RT‐PCR, bacteria were identified by aerobic culture, and Mycoplasma bovis (M. bovis) isolates were speciated by PCR. Agreement among TTW and NS, NPS, or BAL was evaluated by calculating the kappa statistic and percent positive agreement. McNemar's exact test was used to compare the proportions of positive results. RESULTS: Agreement among TTW and NS, TTW and NPS, and TTW and BAL, was very good for identification of P. multocida, M. haemolytica, and M. bovis. For bovine respiratory syncytial virus (BRSV), agreement with TTW was moderate for NS, good for NPS, and very good for BAL. For bovine coronavirus (BCV), agreement with TTW was moderate for NS and NPS, and good for BAL. McNemar's test was significant only for BCV, indicating that for this pathogen the proportion of positive results from NS and NPS could not be considered comparable to TTW. CONCLUSIONS AND CLINICAL IMPORTANCE: This study provides guidance for veterinarians selecting diagnostic tests for antemortem identification of pathogens associated with BRD.
has issue date	2017-03-14(xsd:dateTime)
bibo:doi	10.1111/jvim.14683
bibo:pmid	28295570
has license	cc-by-nc
sha1sum (hex)	044d6ebfdeacd8fbdd4f039872020cd4adbbe138
schema:url	https://doi.org/10.1111/jvim.14683
resource representing a document's title	Agreement Among 4 Sampling Methods to Identify Respiratory Pathogens in Dairy Calves with Acute Bovine Respiratory Disease
has PubMed Central identifier	PMC5434980
has PubMed identifier	28295570
schema:publication	J Vet Intern Med
resource representing a document's body	covid:044d6ebfdeacd8fbdd4f039872020cd4adbbe138#body_text
is schema:about of	named entity 'THESE' named entity 'IDENTIFICATION' named entity 'SAMPLING TECHNIQUES' named entity 'METHODS' named entity 'GUARDED' named entity 'METHODS' named entity 'AGREEMENT' named entity 'TRANSTRACHEAL' named entity 'WASH' named entity 'RESPIRATORY PATHOGENS' named entity 'DAIRY CALVES' named entity 'PATHOGENS' named entity 'ACUTE' named entity 'BOVINE RESPIRATORY DISEASE' named entity 'TO IDENTIFY' named entity 'SAMPLING' named entity 'ANTEMORTEM' named entity 'BOVINE RESPIRATORY DISEASE' named entity 'NASOPHARYNGEAL SWAB' named entity 'USED' named entity 'CHARACTERIZED' named entity 'CATTLE' named entity 'BRONCHOALVEOLAR LAVAGE' named entity 'AGREEMENT' named entity 'NASAL SWAB' named entity 'BACKGROUND' covid:arg/044d6ebfdeacd8fbdd4f039872020cd4adbbe138 named entity 'identification' named entity 'cattle' named entity 'commonly' named entity 'BRD' named entity 'bovine respiratory disease' named entity 'Bovine' named entity 'transtracheal' named entity 'sampling techniques' named entity 'bovine respiratory disease' named entity 'signs of disease' named entity 'Institutional Animal Care and Use Committee' named entity 'P. multocida' named entity 'nostril' named entity 'sample size' named entity 'M. bovis' named entity 'Brucella' named entity 'Mycoplasma' named entity 'lung' named entity 'transtracheal' named entity 'Mycoplasma' named entity 'morbidity' named entity 'dairy calves' named entity 'nasal swab' named entity 'bacteria' named entity 'bovine' named entity 'commensal organisms' named entity 'bovine coronavirus' named entity 'kappa statistic' named entity 'statistical significance' named entity 'P. multocida' named entity 'glycerol' named entity 'Bibersteinia' named entity 'exact test' named entity 'Mycoplasma bovis' named entity 'microbial' named entity 'lower airway' named entity 'flunixin meglumine' named entity 'real-time RT-PCR' named entity 'modified live' named entity 'lower airway' named entity 'isopropanol' named entity 'mucosa' named entity 'diagnostic tests' named entity 'cattle'

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