About: Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum/plasma of cats to diagnose feline infectious peritonitis

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About: Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum/plasma of cats to diagnose feline infectious peritonitis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum/plasma of cats to diagnose feline infectious peritonitis
Creator	Pantchev, Nikola Hartmann, Katrin Egberink, Herman Balzer, Hans-Joerg Felten, Sandra Matiasek, Kaspar Wess, Gerhard Leutenegger, Christian
topic	covid:05ab8bd6ff732bea31fa673b450d37b58da78c46#this
Source	Medline; PMC
abstract	BACKGROUND: Feline coronavirus (FCoV) exists as two pathotypes, and FCoV spike gene mutations are considered responsible for the pathotypic switch in feline infectious peritonitis (FIP) pathogenesis. The aim of this study was to evaluate sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction (RT-PCR) specifically designed to detect FCoV spike gene mutations at two nucleotide positions. It was hypothesized that this test would correctly discriminate feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FECV). METHODS: The study included 63 cats with signs consistent with FIP. FIP was confirmed in 38 cats. Twenty-five control cats were definitively diagnosed with a disease other than FIP. Effusion and/or serum/plasma samples were examined by real-time RT-PCR targeting the two FCoV spike gene fusion peptide mutations M1058 L and S1060A using an allelic discrimination approach. Sensitivity, specificity, negative and positive predictive values including 95% confidence intervals (95% CI) were calculated. RESULTS: FIPV was detected in the effusion of 25/59 cats, one of them being a control cat with chronic kidney disease. A mixed population of FIPV/FECV was detected in the effusion of 2/59 cats; all of them had FIP. RT-PCR was negative or the pathotype could not be determined in 34/59 effusion samples. In effusion, sensitivity was 68.6% (95% CI 50.7–83.2), specificity was 95.8% (95% CI 78.9–99.9). No serum/plasma samples were positive for FIPV. CONCLUSIONS: Although specificity of the test in effusions was high, one false positive result occurred. The use of serum/plasma cannot be recommended due to a low viral load in blood.
has issue date	2017-08-02(xsd:dateTime)
bibo:doi	10.1186/s12917-017-1147-8
bibo:pmid	28768514
has license	cc-by
sha1sum (hex)	05ab8bd6ff732bea31fa673b450d37b58da78c46
schema:url	https://doi.org/10.1186/s12917-017-1147-8
resource representing a document's title	Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum/plasma of cats to diagnose feline infectious peritonitis
has PubMed Central identifier	PMC5541694
has PubMed identifier	28768514
schema:publication	BMC Vet Res
resource representing a document's body	covid:05ab8bd6ff732bea31fa673b450d37b58da78c46#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/05ab8bd6ff732bea31fa673b450d37b58da78c46
is schema:about of	named entity 'DETECTING' named entity 'aim' named entity 'coronavirus' named entity 'detect' named entity 'positions' named entity 'mutations' covid:arg/05ab8bd6ff732bea31fa673b450d37b58da78c46 named entity 'AIM' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'FELINE CORONAVIRUS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'TO DETECT' named entity 'DISCRIMINATE' named entity 'CATS' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'SWITCH' named entity 'FELINE INFECTIOUS PERITONITIS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'DIAGNOSE' named entity 'MUTATIONS' named entity 'EVALUATE' named entity 'SPIKE' named entity 'BACKGROUND' named entity 'GENE MUTATIONS' named entity 'TEST' named entity 'FELINE CORONAVIRUS' named entity 'PCR' named entity 'NUCLEOTIDE' named entity 'REAL-TIME' named entity 'CONSIDERED' named entity 'FELINE INFECTIOUS PERITONITIS' named entity 'STUDY' named entity 'RESPONSIBLE' named entity 'SERUM' named entity 'POSITIONS' named entity 'REAL-TIME' named entity 'FELINE INFECTIOUS PERITONITIS VIRUS' named entity 'EFFUSION' named entity 'PATHOGENESIS' named entity 'spike' named entity 'hypothesized' named entity 'real-time' named entity 'FIP' named entity 'RT-PCR' named entity 'polymerase' named entity 'gene mutations' named entity 'gene mutations' named entity 'reverse transcriptase polymerase chain reaction' named entity 'feline infectious peritonitis' named entity 'RT-PCR' named entity 'FCoV' named entity 'FCoV' named entity 'coronavirus' named entity 'reverse transcriptase polymerase chain reaction' named entity 'sensitivity and specificity' named entity 'FIP' named entity 'feline infectious peritonitis virus' named entity 'plasma' named entity 'gene' named entity 'Feline coronavirus' named entity 'serum' named entity 'feline coronavirus' named entity 'pathogenesis' named entity 'Sensitivity and specificity' named entity 'feline infectious peritonitis' named entity 'nucleotide' named entity 'FCoV' named entity 'FIPV' named entity 'virus' named entity 'peritonitis' named entity 'discriminate' named entity 'mutations'

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