About: Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus

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About: Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus
Creator	Liu, Bin Zhu, Fengcai Chi, Ying Ge, Yiyue Qi, Xian Zhou, Minghao Cui, Lunbiao Shi, Zhiyang Zhao, Kangchen Bian, Qian Su, Chuan Zou, Bingjie
topic	covid:05e6e7a72d16a7294079af8e96f5d6b42d8ce51f#this
Source	PMC
abstract	Considering the fatal human victims and economic loss caused by influenza virus infection every year, methodologies for rapid and on-site detection of influenza viruses are urgently needed. LAMP is the most commonly used nucleic acid isothermal amplification technology suitable for on-site use. However, for multiplex LAMP, differentiation of the amplicons derived from multiple targets is still challengeable currently. Here we developed a multiplex RT-LAMP assay for simultaneous amplification of three prominent subtypes of influenza viruses (A/H5, A/H7 and 2009A/H1). The amplicons were further identified by cascade invasive reaction and nanoparticle hybridization in separate target-specific detection tubes (referred to as mRT-LAMP-IRNH). The analytic sensitivities of the assay are 10 copies of RNA for all the three HA subtypes, and the specificity reached 100%. Clinical specimen analysis showed this assay had a combined sensitivity and specificity of 98.1% and 100%, respectively. Overall, the mRT-LAMP-IRNH assay can be used as a cost-saving method that utilizes a simple instrument to detect A/H5, A/H7, and 2009A/H1 influenza viruses, especially in resource-limited settings.
has issue date	2017-03-21(xsd:dateTime)
bibo:doi	10.1038/srep44924
bibo:pmid	28322309
has license	cc-by
sha1sum (hex)	05e6e7a72d16a7294079af8e96f5d6b42d8ce51f
schema:url	https://doi.org/10.1038/srep44924
resource representing a document's title	Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus
has PubMed Central identifier	PMC5359610
has PubMed identifier	28322309
schema:publication	Sci Rep
resource representing a document's body	covid:05e6e7a72d16a7294079af8e96f5d6b42d8ce51f#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/05e6e7a72d16a7294079af8e96f5d6b42d8ce51f
is schema:about of	named entity 'Here' named entity 'technology' named entity 'rapid' named entity 'MULTIPLEX' named entity 'CASCADE' named entity 'MULTIPLE' named entity 'METHODOLOGIES' named entity 'PROMINENT' named entity '28A' named entity 'COMBINED' named entity 'IDENTIFIED BY' named entity 'COST' covid:arg/05e6e7a72d16a7294079af8e96f5d6b42d8ce51f named entity 'DERIVED' named entity 'RAPID' named entity 'RESOURCE' named entity 'TARGET' named entity 'DIFFERENTIATION' named entity 'NUCLEIC ACID' named entity 'DETECTION' named entity 'LAMP' named entity 'SUBTYPES' named entity 'CASCADE' named entity 'SIMULTANEOUS' named entity 'REVERSE-TRANSCRIPTION' named entity 'INFLUENZA A VIRUS' named entity 'REACTION' named entity 'INVASIVE' named entity 'SPECIFIC' named entity 'COPIES' named entity 'TECHNOLOGY' named entity 'USED' named entity 'TARGETS' named entity 'INSTRUMENT' named entity 'USE' named entity 'METHOD' named entity 'TO DETECT' named entity 'SEPARATE' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'SIMPLE' named entity 'MULTIPLEX' named entity 'HYBRIDIZATION' named entity 'ANALYSIS' named entity 'NEEDED' named entity 'SETTINGS' named entity 'CURRENTLY' named entity 'INFLUENZA VIRUSES' named entity 'RNA' named entity 'RT-LAMP ASSAY' named entity 'LIMITED' named entity 'SITE' named entity 'MRT' named entity '100%' named entity 'REACTION' named entity 'HERE' named entity 'YEAR' named entity 'INVASIVE' named entity 'ASSAY' named entity 'TUBES' named entity 'AMPLIFICATION' named entity 'OVERALL' named entity 'SUITABLE' named entity 'NANOPARTICLE' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'COUPLED' named entity 'HYBRIDIZATION' named entity 'CLINICAL SPECIMEN' named entity 'NANOPARTICLE'

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