About: A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
Creator	Van Gucht, Steven Broeders, Sylvia Dupont-Rouzeyrol, Myrielle Fraiture, Marie-Alice Garlant, Linda Roosens, Nancy Rousset, Dominique Suin, Vanessa Vandermassen, Els Vanhomwegen, Jessica
source	Elsevier; Medline; PMC
abstract	OBJECTIVE: The re-emergence and spread of tropical viruses to new areas has raised a wave of concern worldwide. In order to treat patients at an early stage and prevent the diffusion of an outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) and chikungunya (CHIKV) viruses simultaneously. METHODS: Two methods targeting different genome segments were selected from the literature for each virus. These were adapted for high genome coverage and combined in a four-plex assay that was thoroughly validated in-house. The SCREENED tool was used to evaluate the sequence coverage of the method. RESULTS: The full validation approach showed that the new four-plex method allows the specific and sensitive identification and discrimination of ZIKV and CHIKV in routine samples. The combination of two targets per virus allowing almost 100% coverage of about 500 genomes is shown for the first time. CONCLUSIONS: PCR is a reliable user-friendly technique that can be applied in remote areas. Such multiplex methods enable early and efficient diagnosis, leading to rapid treatment and effective confinement in outbreak cases. They may also serve as an aid for surveillance, and the full validation permits easy method-transfer allowing worldwide harmonization.
has issue date	2019-12-26(xsd:dateTime)
bibo:doi	10.1016/j.ijid.2019.12.028
bibo:pmid	31884173
has license	no-cc
sha1sum (hex)	05ea31d971a2dfb0fc8e6f9ab2e0fdcad1239712
schema:url	https://doi.org/10.1016/j.ijid.2019.12.028
resource representing a document's title	A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
has PubMed Central identifier	PMC7129992
has PubMed identifier	31884173
schema:publication	Int J Infect Dis
resource representing a document's body	covid:05ea31d971a2dfb0fc8e6f9ab2e0fdcad1239712#body_text
is schema:about of	named entity 'CHIKUNGUNYA' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'EARLY STAGE' named entity 'SEQUENCE COVERAGE' named entity 'ABOUT' named entity 'EFFICIENT' named entity 'PREVENT' named entity 'IS A' named entity 'DETECTION' named entity 'SURVEILLANCE' named entity 'GENOME COVERAGE' named entity 'PATIENTS' named entity 'FRIENDLY' named entity 'SENSITIVE' named entity 'ALLOWING' named entity 'COMBINATION' named entity 'EFFECTIVE' named entity 'ENABLE' named entity 'CONCLUSIONS' named entity 'HIGH' named entity 'DIFFERENT' named entity 'SIMULTANEOUSLY' named entity 'THOROUGHLY' named entity 'IN-HOUSE' named entity 'TARGETING' named entity 'EVALUATE' named entity 'PERMITS' named entity '500' named entity 'THESE' named entity 'END' named entity 'AID' named entity 'WORLDWIDE' named entity 'A WAVE' named entity 'ALMOST' named entity 'TO DETECT' named entity 'ZIKA' named entity 'COMBINED' named entity 'LITERATURE' named entity 'ORDER' named entity 'ADEQUATE' named entity 'DIFFUSION' named entity 'SPECIFIC' named entity 'DIAGNOSIS' named entity 'SAMPLES' named entity 'CASES' named entity 'CHIKUNGUNYA' named entity 'TOOL' named entity 'REMOTE AREAS' named entity 'APPLIED' named entity 'VIRUSES' named entity 'ROUTINE' named entity 'AREAS' named entity 'USER' named entity 'TARGETS' named entity '100%' named entity 'PCR' named entity 'EARLY' named entity 'METHOD' named entity 'GENOME' named entity 'NEW' named entity 'ASSAY' named entity 'COVERAGE' named entity 'NEEDED' named entity 'RELIABLE' named entity 'SELECTED' named entity 'EASY' named entity 'MULTIPLEX' named entity 'APPROACH' named entity 'SERVE' named entity 'SIMULTANEOUS' named entity 'ZIKA' named entity 'METHOD'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software