About: Identification and genetic characterization of porcine hemagglutinating encephalomyelitis virus from domestic piglets in China

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About: Identification and genetic characterization of porcine hemagglutinating encephalomyelitis virus from domestic piglets in China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification and genetic characterization of porcine hemagglutinating encephalomyelitis virus from domestic piglets in China
Creator	Gao, Feng Gao, Wei Lan, Yungang Lu, Huijun Zhao, Kui Tang, Bo Dong, Bo Zhao, Jiakuan Liu, Wenfeng Deguang, • Wenqi, Song
Source	Medline; PMC
abstract	In this study, we investigated an acute outbreak of porcine hemagglutinating encephalomyelitis on a farm of 127 pigs in Jilin province, China. Porcine hemagglutinating encephalomyelitis virus (PHEV) was detected in suckling and weaning pigs by RT-PCR assays. Coronavirus-like particles were observed by electron microscopy. The virus isolate was designated PHEV-JT06. The clinical signs, nervous symptoms and positive labeling of neurons in the cerebral cortex with an immunohistochemical stain in PHEV-JT06-infected BALB/c mice supported the diagnosis of PHEV infection. The five full-length PHEV-JT06 structural genes were cloned, sequenced and analyzed. Phylogenetic studies based on the nucleotide and amino acid sequences of the five genes in the outbreak showed that PHEV remained genetically stable. PHEV shares 95.3-99.3 % amino acid sequence identity with American strains (AY078417), suggesting that the Chinese isolate is most likely derived from the North American strain. Additionally, PHEV, HCoV-OC43 and BCoV were genetically close. These results may provide some insights into the genotype of the etiological agent responsible for the porcine hemagglutinating encephalomyelitis outbreak and could also provide a comparative view of the genomics of the five structural proteins of PHEV.
has issue date	2014-04-23(xsd:dateTime)
bibo:doi	10.1007/s00705-014-2070-y
bibo:pmid	24756345
has license	no-cc
sha1sum (hex)	068d29de8bce9086f78d5190df43c5f828e47314
schema:url	https://doi.org/10.1007/s00705-014-2070-y
resource representing a document's title	Identification and genetic characterization of porcine hemagglutinating encephalomyelitis virus from domestic piglets in China
has PubMed Central identifier	PMC7087033
has PubMed identifier	24756345
schema:publication	Arch Virol
resource representing a document's body	covid:068d29de8bce9086f78d5190df43c5f828e47314#body_text
is schema:about of	named entity 'These' named entity 'PHEV' named entity 'Dong' named entity 'PHEV' named entity 'provide' named entity 'symptoms' named entity 'hemagglutinating' named entity 'Jilin' named entity 'China' named entity 'cloned' named entity 'isolate' named entity 'studies' named entity 'PHEV' named entity 'porcine' named entity 'hemagglutinating' named entity 'PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS' named entity 'AMINO ACID SEQUENCE' named entity 'RESPONSIBLE' named entity 'LENGTH' named entity 'CHINESE' named entity 'PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS' named entity 'PARTICLES' named entity 'RESULTS' named entity 'PORCINE' named entity 'ENCEPHALOMYELITIS' named entity 'STRAIN' named entity 'ANALYZED' named entity 'STABLE' named entity 'JILIN PROVINCE' named entity 'BASED' named entity 'RT-PCR' named entity 'IDENTIFICATION' named entity 'HCOV-OC43' named entity 'CEREBRAL CORTEX' named entity 'SUPPORTED' named entity 'DETECTED' named entity 'LABELING' named entity 'AMERICAN' named entity 'NORTH AMERICAN' named entity 'VIEW' named entity 'ELECTRON MICROSCOPY' named entity 'INSIGHTS' named entity 'CORONAVIRUS' named entity 'INFECTED' named entity 'IMMUNOHISTOCHEMICAL STAIN' named entity 'ISOLATE' named entity 'GENOTYPE' named entity 'STUDIES' named entity 'GENES' named entity 'NEURONS' named entity 'CHINA' named entity 'WEANING' named entity 'VIRUS' named entity 'AGENT' named entity 'STRUCTURAL GENES' named entity 'GENETIC CHARACTERIZATION' named entity 'CHINA' named entity 'MOST LIKELY' named entity 'CLINICAL SIGNS' named entity 'POSITIVE' named entity 'OUTBREAK' named entity 'STRUCTURAL PROTEINS' named entity 'NERVOUS SYMPTOMS' named entity 'DONG' named entity 'GENOMICS' named entity 'PROVIDE' named entity 'FARM' named entity 'ACUTE' named entity 'STUDY' named entity 'DESIGNATED' named entity 'SHARES'

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