About: Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)

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About: Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)
Creator	Dübel, Stefan Hust, Michael Hülseweh, Birgit Marschall, Hans-Jürgen Nacke, Christoph Rülker, Torsten Schirrmann, Thomas Kirsch, Martina
topic	covid:06cf324e3d0f793e5c722894c6ea6949655b1f10#this
Source	Medline; PMC
abstract	BACKGROUND: Venezuelan equine encephalitis virus (VEEV) belongs to the Alphavirus group. Several species of this family are also pathogenic to humans and are recognized as potential agents of biological warfare and terrorism. The objective of this work was the generation of recombinant antibodies for the detection of VEEV after a potential bioterrorism assault or an natural outbreak of VEEV. RESULTS: In this work, human anti-VEEV single chain Fragments variable (scFv) were isolated for the first time from a human naïve antibody gene library using optimized selection processes. In total eleven different scFvs were identified and their immunological specificity was assessed. The specific detection of the VEEV strains TC83, H12/93 and 230 by the selected antibody fragments was proved. Active as well as formalin inactivated virus particles were recognized by the selected antibody fragments which could be also used for Western blot analysis of VEEV proteins and immunohistochemistry of VEEV infected cells. The anti-VEEV scFv phage clones did not show any cross-reactivity with Alphavirus species of the Western equine encephalitis virus (WEEV) and Eastern equine encephalitis virus (EEEV) antigenic complex, nor did they react with Chikungunya virus (CHIKV), if they were used as detection reagent. CONCLUSION: For the first time, this study describes the selection of antibodies against a human pathogenic virus from a human naïve scFv antibody gene library using complete, active virus particles as antigen. The broad and sensitive applicability of scFv-presenting phage for the immunological detection and diagnosis of Alphavirus species was demonstrated. The selected antibody fragments will improve the fast identification of VEEV in case of a biological warfare or terroristic attack or a natural outbreak.
has issue date	2008-09-02(xsd:dateTime)
bibo:doi	10.1186/1472-6750-8-66
bibo:pmid	18764933
has license	cc-by
sha1sum (hex)	06cf324e3d0f793e5c722894c6ea6949655b1f10
schema:url	https://doi.org/10.1186/1472-6750-8-66
resource representing a document's title	Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)
has PubMed Central identifier	PMC2543005
has PubMed identifier	18764933
schema:publication	BMC Biotechnol
resource representing a document's body	covid:06cf324e3d0f793e5c722894c6ea6949655b1f10#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/06cf324e3d0f793e5c722894c6ea6949655b1f10
is schema:about of	named entity 'PHAGE DISPLAY' named entity 'HUMAN' covid:arg/06cf324e3d0f793e5c722894c6ea6949655b1f10 named entity 'BIOTERRORISM' named entity 'PATHOGENIC' named entity 'RECOMBINANT ANTIBODIES' named entity 'NATURAL' named entity 'FAMILY' named entity 'ALPHAVIRUS' named entity 'BACKGROUND' named entity 'GROUP' named entity 'WORK' named entity 'POTENTIAL' named entity 'BIOLOGICAL WARFARE' named entity 'OBJECTIVE' named entity 'HUMANS' named entity 'VENEZUELAN EQUINE ENCEPHALITIS VIRUS' named entity 'OUTBREAK' named entity 'USING' named entity 'DETECTION' named entity 'ASSAULT' named entity 'FRAGMENTS' named entity 'ANTIBODY' named entity 'DIAGNOSIS' named entity 'DETECTION' named entity 'SPECIES' named entity 'TERRORISM' named entity 'VENEZUELAN EQUINE ENCEPHALITIS VIRUS' named entity 'DEVELOPMENT' named entity 'GENERATION' named entity 'recombinant antibodies' named entity 'biological warfare' named entity 'VEEV' named entity 'Venezuelan equine encephalitis virus' named entity 'antibody' named entity 'fetal calf serum' named entity 'VEE' named entity 'cell culture' named entity 'Fab' named entity 'light chain' named entity 'mAb' named entity 'biowarfare' named entity 'zoonoses' named entity 'immunoblot' named entity 'Staining' named entity 'antigen' named entity 'Venezuelan equine encephalitis virus' named entity 'vaccine' named entity 'scFv' named entity 'positive controls' named entity 'Western equine encephalitis virus' named entity 'phage' named entity 'Alphavirus' named entity 'staining' named entity 'scFv' named entity 'Chemicon' named entity '37°C' named entity 'scFv' named entity 'biosafety level 3' named entity 'scFv' named entity 'centrifugation' named entity 'vaccination' named entity 'BSA' named entity 'scFv' named entity 'antibody' named entity 'scFv' named entity 'VEEV' named entity 'antibodies' named entity 'mAb' named entity 'phage' named entity 'virus' named entity 'scFv'

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