About: Molecular characterization of genome segment 2 encoding RNA dependent RNA polymerase of Antheraea mylitta cytoplasmic polyhedrosis virus

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About: Molecular characterization of genome segment 2 encoding RNA dependent RNA polymerase of Antheraea mylitta cytoplasmic polyhedrosis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of genome segment 2 encoding RNA dependent RNA polymerase of Antheraea mylitta cytoplasmic polyhedrosis virus
Creator	Bagchi, Abhisek Chakrabarti, Mrinmay Chavali, Murthy Ghorai, Suvankar Kumar, Ananta Ramana, Venkata Roy, Sobhan
topic	covid:075f3a5c06f30b65661f169c9748c9ce90e89b72#this
Source	Elsevier; Medline; PMC
abstract	Abstract Genome segment 2 (S2) from Antheraea mylitta cypovirus (AmCPV) was converted into cDNA, cloned and sequenced. S2 consisted of 3798 nucleotides with a long ORF encoding a 1116 amino acid long protein (123kDa). BLAST and phylogenetic analysis showed 29% sequence identity and close relatedness of AmCPV S2 with RNA dependent RNA polymerase (RdRp) of other insect cypoviruses, suggesting a common origin of all insect cypoviruses. The ORF of S2 was expressed as 123kDa soluble His-tagged fusion protein in insect cells via baculovirus recombinants which exhibited RdRp activity in an in vitro RNA polymerase assay without any intrinsic terminal transferase activity. Maximum activity was observed at 37°C at pH 6.0 in the presence of 3mM MgCl2. Site directed mutagenesis confirmed the importance of the conserved GDD motif. This is the first report of functional characterization of a cypoviral RdRp which may lead to the development of anti-viral agents.
has issue date	2010-08-15(xsd:dateTime)
bibo:doi	10.1016/j.virol.2010.04.019
bibo:pmid	20488502
has license	els-covid
sha1sum (hex)	075f3a5c06f30b65661f169c9748c9ce90e89b72
schema:url	https://doi.org/10.1016/j.virol.2010.04.019
resource representing a document's title	Molecular characterization of genome segment 2 encoding RNA dependent RNA polymerase of Antheraea mylitta cytoplasmic polyhedrosis virus
has PubMed Central identifier	PMC7111928
has PubMed identifier	20488502
schema:publication	Virology
resource representing a document's body	covid:075f3a5c06f30b65661f169c9748c9ce90e89b72#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/075f3a5c06f30b65661f169c9748c9ce90e89b72
is schema:about of	named entity 'ORF' named entity 'transferase' covid:arg/075f3a5c06f30b65661f169c9748c9ce90e89b72 named entity 'long' named entity 'intrinsic' named entity 'Antheraea mylitta' named entity 'expressed' named entity 'sequence identity' named entity 'His-tagged' named entity 'fusion protein' named entity 'baculovirus' named entity 'nucleotides' named entity 'cDNA' named entity 'polyhedrosis' named entity 'Antheraea mylitta' named entity 'genome' named entity 'Bacterial cells' named entity 'virus' named entity 'sonicated' named entity 'RNA synthesis' named entity 'HCV' named entity 'protein' named entity 'protein' named entity 'RNA' named entity 'base pair' named entity 'Ni-NTA' named entity 'Phylogenetic comparison' named entity 'RdRp' named entity 'Hymenoptera' named entity 'protein' named entity 'agarose gel' named entity 'digestion' named entity 'ATPase' named entity 'NaCl' named entity 'RNA' named entity 'RNA' named entity 'Reoviridae' named entity 'genome' named entity 'genus' named entity 'M13' named entity 'polymerization reaction' named entity 'homology' named entity 'RdRp' named entity 'μCi' named entity 'wild type' named entity 'ORF' named entity 'nucleotides' named entity 'open reading frame' named entity 'enzyme' named entity 'pathogens' named entity 'India' named entity 'Oligo' named entity 'pmol' named entity 'RNA' named entity 'Reoviridae' named entity 'image analysis' named entity 'double stranded RNA' named entity 'helicase' named entity 'silkworms' named entity 'polyclonal antibody' named entity 'ORF' named entity 'A. mylitta' named entity 'positive strand' named entity 'RNA' named entity 'silkworm' named entity 'protein' named entity 'RdRp' named entity '2.2' named entity 'Genbank' named entity 'Invitrogen' named entity 'ribonucleotide' named entity 'viral DNA' named entity 'viral genome replication'

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