About: Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex
Creator	Bae, Joon-Yong Park, Man-Seong Kim, Jinsoo Kwon, Hyung-Joo Maharjan, Sony Lee, Su Park, Byoung
source	Medline; PMC
abstract	Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future.
has issue date	2019-06-30(xsd:dateTime)
bibo:doi	10.5483/bmbrep.2019.52.6.185
bibo:pmid	30355437
has license	cc-by-nc
sha1sum (hex)	077a70059a89059bace1150d779cc911a2229c60
schema:url	https://doi.org/10.5483/bmbrep.2019.52.6.185
resource representing a document's title	Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex
has PubMed Central identifier	PMC6605520
has PubMed identifier	30355437
schema:publication	BMB Rep
resource representing a document's body	covid:077a70059a89059bace1150d779cc911a2229c60#body_text
is schema:about of	named entity 'REDUCED' named entity '492' named entity 'PLAQUE' named entity 'IGG' named entity 'WESTERN BLOTTING' named entity 'REACTIVITY' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'DOMAIN' named entity 'APPLICATIONS' named entity 'PROTEIN ' named entity 'VIRUS' named entity 'COMPLEX' named entity 'MONOCLONAL ANTIBODY' named entity 'ANTIBODIES' named entity 'COULD BE' named entity 'MOUSE' named entity 'STRONG' named entity 'Rep' named entity 'western blotting' named entity 'antibodies' named entity 'produced' named entity 'reactivity' named entity 'detected' named entity 'LIPOSOME' named entity 'MERS-COV' named entity 'COMPLEX' named entity 'SYNTHETIC PEPTIDE' named entity 'MERS-COV' named entity 'NATIVE FORM' named entity 'DIRECTED' named entity 'EPITOPE PEPTIDE' named entity 'THERAPEUTIC' named entity 'USED' named entity 'S PROTEIN' named entity 'CPG' named entity 'LIPOSOME' named entity 'SPIKE PROTEIN' named entity 'USING' named entity 'TARGETING' named entity 'DNA' named entity 'EPITOPE' named entity 'MONOCLONAL ANTIBODY' named entity 'CPG' named entity 'CHARACTERIZATION' named entity 'MONOCLONAL ANTIBODIES' named entity 'NORMAL' named entity 'MERS-COV S PROTEIN' named entity 'RBD' named entity 'FORMATION' named entity 'DIAGNOSTICS' named entity 'DEVELOPMENT' named entity 'PROTEIN ' named entity 'WWW' named entity 'REPORTS' named entity 'VERO CELLS' named entity 'GENERATION' named entity 'DNA' named entity 'ENCAPSULATED' named entity 'MICE' named entity 'DETECTED' named entity 'FUTURE' named entity 'COMPARED' named entity 'BMB' named entity 'ANALYSIS' named entity 'DATA' named entity 'INFECTED' named entity 'RECEPTOR BINDING' named entity 'REP' named entity 'ORG' named entity '2019' covid:arg/077a70059a89059bace1150d779cc911a2229c60 named entity 'protein' named entity 'virus' named entity 'MERS-CoV' named entity 'protein'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software