About: Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media

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About: Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media
Creator	Bruzzone, Chiara Bragado, María Fernández-Hernández, Pablo González-Fernández, Lauro Gutiérrez-Adán, Alfonso Macías-García, Beatriz Millet, Óscar Jesús García-Marín, Luis Jesús Sánchez-Calabuig, María
source	PMC
abstract	SIMPLE SUMMARY: Commercial in vitro embryo production in horses by ICSI (intracytoplasmic sperm injection) is currently used to produce embryos clinically. However, the successful pregnancy and foaling rates obtained after ICSI are only 10% of the oocytes matured in vitro. Conditions used for oocyte in vitro maturation are not optimized for equine oocytes. Hence, in the present work, we aimed to elucidate the major metabolites present in equine preovulatory follicular fluid obtained from postmortem mares using proton nuclear magnetic resonance spectroscopy ((1)H-NMR). Twenty-two metabolites were identified; among these, nine of them are not included in the composition of in vitro maturation media. Hence, our data suggest that the currently used media for equine oocyte maturation can be further improved. ABSTRACT: Production of equine embryos in vitro is currently a commercial technique and a reliable way of obtaining offspring. In order to produce those embryos, immature oocytes are retrieved from postmortem ovaries or live mares by ovum pick-up (OPU), matured in vitro (IVM), fertilized by intracytoplasmic sperm injection (ICSI), and cultured until day 8–10 of development. However, at best, roughly 10% of the oocytes matured in vitro and followed by ICSI end up in successful pregnancy and foaling, and this could be due to suboptimal IVM conditions. Hence, in the present work, we aimed to elucidate the major metabolites present in equine preovulatory follicular fluid (FF) obtained from postmortem mares using proton nuclear magnetic resonance spectroscopy ((1)H-NMR). The results were contrasted against the composition of the most commonly used media for equine oocyte IVM: tissue culture medium 199 (TCM-199) and Dulbecco’s modified eagle medium/nutrient mixture F-12 Ham (DMEM/F-12). Twenty-two metabolites were identified in equine FF; among these, nine of them are not included in the composition of DMEM/F-12 or TCM-199 media, including (mean ± SEM): acetylcarnitine (0.37 ± 0.2 mM), carnitine (0.09 ± 0.01 mM), citrate (0.4 ± 0.04 mM), creatine (0.36 ± 0.14 mM), creatine phosphate (0.36 ± 0.05 mM), fumarate (0.05 ± 0.007 mM), glucose-1-phosphate (6.9 ± 0.4 mM), histamine (0.25 ± 0.01 mM), or lactate (27.3 ± 2.2 mM). Besides, the mean concentration of core metabolites such as glucose varied (4.3 mM in FF vs. 5.55 mM in TCM-199 vs. 17.5 mM in DMEM/F-12). Hence, our data suggest that the currently used media for equine oocyte IVM can be further improved.
has issue date	2020-05-19(xsd:dateTime)
bibo:doi	10.3390/ani10050883
bibo:pmid	32438699
has license	cc-by
sha1sum (hex)	07fb26cffbd85278f0f5fbf4273b852b2d33bd52
schema:url	https://doi.org/10.3390/ani10050883
resource representing a document's title	Study of the Metabolomics of Equine Preovulatory Follicular Fluid: A Way to Improve Current In Vitro Maturation Media
has PubMed Central identifier	PMC7278476
has PubMed identifier	32438699
schema:publication	Animals (Basel)
resource representing a document's body	covid:07fb26cffbd85278f0f5fbf4273b852b2d33bd52#body_text
is schema:about of	named entity 'maturation' named entity 'postmortem' named entity 'Study' named entity 'Fluid' named entity 'Metabolomics' named entity 'metabolites' named entity 'foaling' named entity 'Summary' named entity 'identified' named entity 'oocyte' named entity 'Equine' named entity 'oocyte' named entity 'metabolites' named entity 'oocyte maturation' named entity 'proton nuclear magnetic resonance' named entity 'H-NMR' named entity 'follicular fluid' named entity 'Follicular Fluid' named entity 'metabolism' named entity 'DMEM' named entity 'metabolism' named entity 'follicles' named entity 'valine' named entity 'fumarate' named entity 'lactate' named entity 'TCA' named entity 'nuclear magnetic resonance' named entity 'embryos' named entity 'metabolic' named entity 'descriptive statistics' named entity 'mares' named entity 'hypodermic needle' named entity 'methanol' named entity 'preovulatory' named entity 'amino acids' named entity 'alanine' named entity 'carnitine' named entity 'reactive oxygen species' named entity 'H-NMR' named entity 'DMEM' named entity 'oocytes' named entity 'lipoproteins' named entity 'mitochondrial oxidative phosphorylation' named entity 'bovine' named entity 'metabolism of glucose' named entity 'oocytes' named entity 'Bruker' named entity 'chemical shifts' named entity 'glycoconjugates' named entity 'postmortem' named entity 'arginine' named entity 'glycogen' named entity 'intracellular' named entity 'glycogen' named entity 'centrifuged' named entity 'OPU' named entity 'proton' named entity 'glycogen' named entity 'acetate' named entity 'metabolism' named entity 'follicles' named entity 'DMEM' named entity 'oocyte' named entity 'follicular fluid' named entity 'arginine' named entity 'citrate' named entity 'lactate' named entity 'amino acid' named entity 'lyophilized' named entity 'oocytes' named entity 'uterine' named entity 'meiotic' named entity 'FBS'

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