About: Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR
Creator	Gromadzka, Beata Nidzworski, Dawid Rabalski, Lukasz
Source	Elsevier; Medline; PMC
abstract	A rapid diagnostic method based on the melting curve SYBR Green I real-time PCR analysis was developed to detect and differentiate Newcastle disease virus (NDV) strains. Degenerated primers based on the cleavage site sequence of the F0 gene were designed to detect specific sequences characteristic of virulent and avirulent strains of NDV. Eighteen strains of NDV from four lineages were identified and grouped into virulent and avirulent strains. Peaks on the melting temperature graph with melting temperature values between 80.00 and 83.80 °C were observed for lentogenic (avirulent) strains. T(m) values higher than 83.80 were observed for virulent (mesogenic and velogenic) strains. The detection limit of real-time PCR was 2 × 10(2) plasmid copies per reaction or 10(2) EID(50) for velogenic strains and 10(3) EID(50) for lentogenic strains. The results obtained in this study demonstrate the possible applications for melting curve real-time PCR analysis in laboratory practice for the diagnosis and differentiation of avirulent and virulent strains of Newcastle disease virus.
has issue date	2010-12-28(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.12.015
bibo:pmid	21192979
has license	no-cc
sha1sum (hex)	0825a7b4df2562051386a726f10e5ce14560a69e
schema:url	https://doi.org/10.1016/j.jviromet.2010.12.015
resource representing a document's title	Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR
has PubMed Central identifier	PMC7112782
has PubMed identifier	21192979
schema:publication	J Virol Methods
resource representing a document's body	covid:0825a7b4df2562051386a726f10e5ce14560a69e#body_text
is schema:about of	named entity 'sequence' named entity 'DETECTION' named entity 'STRAINS' named entity 'BASED' named entity 'GENE' named entity 'SEQUENCE' named entity 'RAPID' named entity 'REAL-TIME PCR' named entity 'DIFFERENTIATION' named entity 'PCR ANALYSIS' named entity 'CHARACTERISTIC' named entity 'SYBR GREEN I' named entity 'VIRULENT' named entity 'MELTING CURVE' named entity 'AVIRULENT' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'SPECIFIC' named entity 'AVIRULENT' named entity 'DEGENERATED' named entity 'DIFFERENTIATE' named entity 'CLEAVAGE' named entity 'VIRULENT' named entity 'REAL-TIME PCR ' named entity 'SITE' named entity 'DIAGNOSTIC METHOD' named entity 'SEQUENCES' named entity 'STRAINS' named entity 'TO DETECT' named entity 'PRIMERS' named entity 'NEWCASTLE DISEASE VIRUS' covid:arg/0825a7b4df2562051386a726f10e5ce14560a69e named entity 'based' named entity 'strains' named entity 'gene' named entity 'NDV' named entity 'virulent' named entity 'Newcastle disease' named entity 'real-time PCR' named entity 'NDV' named entity 'NDV' named entity 'viral RNA' named entity 'GenBank' named entity 'KOH' named entity 'reverse transcriptase' named entity 'Poland' named entity 'plasmid DNA' named entity 'Italy' named entity 'NDV' named entity 'Polish' named entity 'real-time PCR' named entity 'cleavage site' named entity 'PCR' named entity 'Poland' named entity 'SYBR Green I' named entity 'virulence' named entity 'respiratory tract' named entity 'spectrophotometrically' named entity 'virulent' named entity 'NDV' named entity 'avirulent' named entity 'neighbor-joining method' named entity 'Australia' named entity 'ICPI' named entity 'non-segmented' named entity 'Germany' named entity 'Newcastle disease' named entity 'Pulawy' named entity 'phylogenetic relationship' named entity 'cDNA' named entity 'avirulent' named entity 'melting curve analysis' named entity 'Transcriptor' named entity 'Gdansk' named entity 'AIV' named entity 'Roche Diagnostics' named entity 'reverse transcriptase' named entity 'bacteria' named entity 'SYBR Green' named entity 'NDV'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software