About: Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014

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About: Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014 - 2015 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014 - 2015
Creator	Hosseini, Hossein Akbarpour, Bijan Charkhkar, Saied Saadat, Yousef Shaikhi, Nariman Dvm, Hassan, Mohammad Fard, Bozorgmehri
Source	Medline; PMC
abstract	The aim of this study was to provide information on the molecular characteristic and the phylogenic relationship of infectious bronchitis viruses (IBV) strains in Bushehr province in comparison to other strains reported in the Middle East. Samples were collected from broiler flocks in Bushehr province during 2014 - 2015. These flocks had respiratory problems such as gasping, sneezing and bronchial rales. A number of 135 tracheal swabs were taken from fifteen flocks (nine swabs per flock). Each three swabs collected from each flock were pooled in one tube (finally, we had three tubes for each flock). The samples were subjected to reverse transcription polymerase chain reaction (RT-PCR). The PCR products of positive samples were analyzed by sequencing of a (392 bp) segment of the spike gene and the related results were compared with the other IBV sequences in GenBank database. Samples from twelve farms (80.0%) were found to be positive. The viruses from seven farms (46.6%) were identified as field viruses closely related to variant 2. The viruses from three farms (20.0%) were characterized as Mass type and were related to vaccine strains. Two different IB viruses (variant 2 and Mass) were detected in samples from two farms (13.3%). The variant 2 genotype detected in Bushehr had high similarity to variant 2 reported from the Middle East. These variants displayed homologies ranging from 72.9% to 76.5%, and 78.8% to 80.0% with H120 and 4/91, respectively. It is necessary to design vaccination program of poultry farms using IBV strains circulating in the region.
has issue date	2017-09-15(xsd:dateTime)
bibo:pmid	29085606
has license	cc-by
sha1sum (hex)	08e6541bee1b67eab1918bcbbe6f90463ecb651a
resource representing a document's title	Molecular characterization of infectious bronchitis viruses isolated from broiler flocks in Bushehr province, Iran: 2014 - 2015
has PubMed Central identifier	PMC5653882
has PubMed identifier	29085606
schema:publication	Vet Res Forum
resource representing a document's body	covid:08e6541bee1b67eab1918bcbbe6f90463ecb651a#body_text
is schema:about of	named entity 'HIGH' named entity 'SEQUENCING' named entity 'DIFFERENT' named entity 'IBV' named entity 'DESIGN' named entity 'GENOTYPE' named entity 'SPIKE' named entity 'PRODUCTS' named entity 'THESE' named entity 'VIRUSES' named entity 'GENBANK' named entity 'FARMS' named entity '28392' named entity 'VACCINE' named entity 'GENE' named entity 'DISPLAYED' named entity 'NUMBER OF' named entity 'MASS' named entity '135' named entity 'IDENTIFIED' named entity 'POSITIVE' named entity 'TUBE' named entity 'VARIANT' named entity 'SNEEZING' named entity 'FIELD' named entity 'DETECTED' named entity 'SIMILARITY' named entity 'RESULTS' named entity 'REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION' named entity 'RESPIRATORY PROBLEMS' named entity 'MOLECULAR' named entity 'RALES' named entity 'INFECTIOUS BRONCHITIS' named entity 'FOUND' named entity 'SEGMENT' named entity 'CHARACTERISTIC' named entity 'COLLECTED' named entity 'COMPARISON' named entity 'PCR' named entity 'TYPE' named entity 'RELATIONSHIP' named entity '80%' named entity 'IRAN' named entity 'ISOLATED' named entity 'VIRUSES' named entity 'CHARACTERIZATION' named entity 'BROILER' named entity 'INFECTIOUS BRONCHITIS' named entity 'BRONCHIAL' named entity 'STUDY' named entity 'CIRCULATING' named entity 'POOLED' named entity 'REPORTED' named entity 'VACCINATION PROGRAM' named entity 'STRAINS' named entity '2015' named entity 'BUSHEHR PROVINCE' named entity 'TAKEN' named entity '2015' named entity 'BUSHEHR PROVINCE' named entity 'POULTRY' named entity 'SWABS' named entity 'USING' named entity 'SAMPLES' named entity 'GASPING' named entity 'RELATED' named entity 'SEQUENCES' named entity 'MOLECULAR' named entity 'BROILER' named entity 'TO PROVIDE' named entity 'NECESSARY' named entity 'TRACHEAL' named entity '2880' named entity 'SEVEN' named entity 'CHARACTERIZED' named entity 'BUSHEHR'

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