About: Establishment, Immortalisation and Characterisation of Pteropid Bat Cell Lines

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Establishment, Immortalisation and Characterisation of Pteropid Bat Cell Lines
Creator	Wang, Lin-Fa Crameri, Gary Middleton, Deborah Yu, Meng Field, Hume Tachedjian, Mary Todd, Shawn Bulach, Dieter De Jong, Carol Grimley, Samantha Liu, Jun-Ping Smith, Craig Marsh, Glenn Mceachern, Jennifer Michalski, Wojtek Virtue, Elena
topic	covid:08e78c0222e84457195ccefb2e371f1847670436#this
Source	Medline; PMC
abstract	BACKGROUND: Bats are the suspected natural reservoir hosts for a number of new and emerging zoonotic viruses including Nipah virus, Hendra virus, severe acute respiratory syndrome coronavirus and Ebola virus. Since the discovery of SARS-like coronaviruses in Chinese horseshoe bats, attempts to isolate a SL-CoV from bats have failed and attempts to isolate other bat-borne viruses in various mammalian cell lines have been similarly unsuccessful. New stable bat cell lines are needed to help with these investigations and as tools to assist in the study of bat immunology and virus-host interactions. METHODOLOGY/FINDINGS: Black flying foxes (Pteropus alecto) were captured from the wild and transported live to the laboratory for primary cell culture preparation using a variety of different methods and culture media. Primary cells were successfully cultured from 20 different organs. Cell immortalisation can occur spontaneously, however we used a retroviral system to immortalise cells via the transfer and stable production of the Simian virus 40 Large T antigen and the human telomerase reverse transcriptase protein. Initial infection experiments with both cloned and uncloned cell lines using Hendra and Nipah viruses demonstrated varying degrees of infection efficiency between the different cell lines, although it was possible to infect cells in all tissue types. CONCLUSIONS/SIGNIFICANCE: The approaches developed and optimised in this study should be applicable to bats of other species. We are in the process of generating further cell lines from a number of different bat species using the methodology established in this study.
has issue date	2009-12-11(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0008266
bibo:pmid	20011515
has license	cc-by
sha1sum (hex)	08e78c0222e84457195ccefb2e371f1847670436
schema:url	https://doi.org/10.1371/journal.pone.0008266
resource representing a document's title	Establishment, Immortalisation and Characterisation of Pteropid Bat Cell Lines
has PubMed Central identifier	PMC2788226
has PubMed identifier	20011515
schema:publication	PLoS One
resource representing a document's body	covid:08e78c0222e84457195ccefb2e371f1847670436#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/08e78c0222e84457195ccefb2e371f1847670436
is schema:about of	named entity 'coronaviruses' named entity 'isolate' named entity 'severe acute respiratory syndrome coronavirus' named entity 'viruses' named entity 'BAT' named entity 'CELL LINES' named entity 'HAVE' named entity 'THESE' named entity 'INCLUDING' named entity 'SARS' named entity 'BACKGROUND' named entity 'SUSPECTED' named entity 'RESERVOIR' named entity 'LIKE' named entity 'EBOLA VIRUS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ISOLATE' named entity 'IMMUNOLOGY' named entity 'MAMMALIAN CELL' named entity 'DISCOVERY' named entity 'CELL LINES' named entity 'STABLE' named entity 'NEEDED' named entity 'VIRUSES' named entity 'VIRUS' named entity 'NEW' named entity 'VARIOUS' named entity 'CORONAVIRUSES' named entity 'HOSTS' named entity 'HENDRA VIRUS' named entity 'BORNE' named entity 'BAT' named entity 'UNSUCCESSFUL' named entity 'NUMBER OF' named entity 'ESTABLISHMENT' named entity 'ZOONOTIC' named entity 'NATURAL' named entity 'BATS' named entity 'CHINESE' named entity 'TOOLS' named entity 'HOST' named entity 'NIPAH VIRUS' named entity 'TO HELP' named entity 'ASSIST' named entity 'INVESTIGATIONS' named entity 'STUDY' named entity 'HORSESHOE BATS' named entity 'INTERACTIONS' named entity 'FAILED' covid:arg/08e78c0222e84457195ccefb2e371f1847670436 named entity 'interactions' named entity 'isolate' named entity 'needed' named entity 'Bats' named entity 'Nipah virus' named entity 'horseshoe bats' named entity 'viruses' named entity 'bat' named entity 'severe acute respiratory syndrome coronavirus' named entity 'serum' named entity 'primary cell' named entity 'fruit bat' named entity 'immune responses' named entity 'cell culture medium'

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