About: Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana

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About: Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana
Creator	Sun, Xiaohong Bai, Suhua Chen, Qiming Dai, Hongyi Dong, Chaohua Zhang, Yugang
Source	PMC
abstract	BACKGROUND: Lysin motif (LysM)-containing proteins are involved in the recognition of fungal and bacterial pathogens. However, few studies have reported on their roles in the defense responses of woody plants against pathogens. A previous study reported that the apple MdCERK1 gene was induced by chitin and Rhizoctonia solani, and its protein can bind to chitin. However, its effect on defense responses has not been investigated. RESULTS: In this study, a new apple CERK gene, designated as MdCERK1–2, was identified. It encodes a protein that shares high sequence identity with the previously reported MdCERK1 and AtCERK1. Its chitin binding ability and subcellular location are similar to MdCERK1 and AtCERK1, suggesting that MdCERK1–2 may play a role in apple immune defense responses as a pattern recognition receptor (PRR). MdCERK1–2 expression in apple was induced by 2 fungal pathogens, Botryosphaeria dothidea and Glomerella cingulate, but not by the bacterial pathogen, Erwinia amylovora, indicating that MdCERK1–2 is involved in apple anti-fungal defense responses. Further functional analysis by heterologous overexpression (OE) in Nicotiana benthamiana (Nb) demonstrated that MdCERK1–2 OE improved Nb resistance to the pathogenic fungus, Alternaria alternata. H(2)O(2) accumulation and callose deposition increased after A. alternata infection in MdCERK1–2 OE plants compared to wild type (WT) and empty vector (EV)-transformed plants. The induced expression of NbPAL4 by A. alternata significantly (p < 0.01, n = 4) increased in MdCERK1–2 OE plants. Other tested genes, including NbNPR1, NbPR1a, NbERF1, and NbLOX1, did not exhibit significant changes after A. alternata infection in OE plants compared to EV or WT plants. OE plants also accumulated more polyphenols after A. alternata infection. CONCLUSIONS: Heterologous MdCERK1–2 OE affects multiple defense responses in Nb plants and increased their resistance to fungal pathogens. This result also suggests that MdCERK1–2 is involved in apple defense responses against pathogenic fungi.
has issue date	2020-04-08(xsd:dateTime)
bibo:doi	10.1186/s12870-020-02361-z
bibo:pmid	32268888
has license	cc-by
sha1sum (hex)	08fac40bb22a79d491bc19c388b6737f334ed430
schema:url	https://doi.org/10.1186/s12870-020-02361-z
resource representing a document's title	Overexpression of an apple LysM-containing protein gene, MdCERK1–2, confers improved resistance to the pathogenic fungus, Alternaria alternata, in Nicotiana benthamiana
has PubMed Central identifier	PMC7386173
has PubMed identifier	32268888
schema:publication	BMC Plant Biol
resource representing a document's body	covid:08fac40bb22a79d491bc19c388b6737f334ed430#body_text
is schema:about of	covid:arg/08fac40bb22a79d491bc19c388b6737f334ed430 named entity 'pathogens' named entity 'Lysin' named entity 'bacterial pathogen' named entity 'gene' named entity 'functional analysis' named entity 'apple' named entity 'chitin' named entity 'gene' named entity 'apple' named entity 'pattern recognition receptor' named entity 'Nicotiana benthamiana' named entity 'protein' named entity 'Alternaria alternata' named entity 'Luminol' named entity 'polyphenolic' named entity 'microscopy' named entity '350 nm' named entity 'phenolic' named entity 'clone' named entity 'fungal pathogens' named entity 'biosynthesis' named entity 'qRT-PCR' named entity 'mycelial growth' named entity 'fungal pathogen' named entity 'aniline blue' named entity 'extracellular region' named entity 'Callose' named entity 'fungal pathogens' named entity 'infection' named entity 'callose' named entity 'phenol' named entity 'bacterial pathogens' named entity 'infection' named entity 'plasma membrane' named entity 'callose' named entity 'fungal pathogen' named entity 'intracellular' named entity 'A. alternata' named entity 'inoculation' named entity 'antibody' named entity 'callose' named entity 'lesion' named entity 'infection' named entity 'qRT-PCR' named entity 'PCR' named entity 'fungal pathogens' named entity 'chitin' named entity 'ectodomain' named entity 'PAMP' named entity 'fungal pathogen' named entity 'Arabidopsis' named entity 'inoculation' named entity 'chitin' named entity 'protein' named entity 'phenotype' named entity 'signaling pathway' named entity 'receptor' named entity 'ectodomain' named entity 'PGN' named entity 'chitin' named entity 'chitin' named entity 'post-hoc test' named entity 'post-hoc test' named entity 'pathogens' named entity '0.01' named entity 'signaling pathways' named entity 'fungal pathogens' named entity 'antibody' named entity 'genome' named entity 'A. alternata' named entity 'genetic transformation' named entity 'rice' named entity 'A. tumefaciens' named entity 'Nicotiana benthamiana' named entity 'Shanghai'

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