About: Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device

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About: Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device
Creator	Chang, Tien-Li Chen, Zhao-Chi Li, Ching-Hao Liu, Cheng-Che Su, Kai-Wen
Source	Elsevier; Medline; PMC
abstract	Abstract Rapid thermal cycling (RTC) in an on-chip device can perform DNA amplification in vitro through precise thermal control at each step of the polymerase chain reaction (PCR). This study reports a straightforward fabrication technique for patterning an on-chip graphene-based device with hole arrays, in which the mechanism of surface structures can achieve stable and uniform thermal control for the amplification of DNA fragments. A thin-film based PCR device was fabricated using picosecond laser (PS-laser) ablation of the multilayer graphene (MLG). Under the optimal fluence of 4.72 J/cm2 with a pulse overlap of 66%, the MLG can be patterned with arrays of 250 μm2 hole surface structures. A 354-bp DNA fragment of VP1, an effective marker for diagnosing the BK virus, was amplified on an on-chip device in less than 60 min. A thin-film electrode with the aforementioned MLG as the heater was demonstrated to significantly enhance temperature stability for each stage of the thermal cycle. The temperature control of the heater was performed by means of a developed programmable PCR apparatus. Our results demonstrated that the proposed integration of a graphene-based device and a laser-pulse ablation process to form a thin-film PCR device has cost benefits in a small-volume reagent and holds great promise for practical medical use of DNA amplification.
has issue date	2019-12-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2019.111581
bibo:pmid	31629228
has license	els-covid
sha1sum (hex)	0938dd5e081c40336508df612842413be32271da
schema:url	https://doi.org/10.1016/j.bios.2019.111581
resource representing a document's title	Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device
has PubMed Central identifier	PMC7126615
has PubMed identifier	31629228
schema:publication	Biosensors and Bioelectronics
resource representing a document's body	covid:0938dd5e081c40336508df612842413be32271da#body_text
is schema:about of	named entity 'DNA' named entity 'benefits' named entity 'thermal control' named entity 'process' named entity 'MLG' named entity 'hole' named entity 'amplification' named entity 'integration' named entity 'thin-film' named entity 'mechanism' named entity 'device' named entity 'DNA' named entity 'uniform' named entity 'amplification' named entity 'THERMAL' named entity 'DNA AMPLIFICATION' named entity 'MIN' named entity 'IN VITRO' named entity 'GREAT' named entity 'DNA' named entity 'USING' named entity 'DNA AMPLIFICATION' named entity 'MEDICAL' named entity 'MEANS' named entity 'STEP' named entity 'CHIP' named entity 'PATTERNING' named entity 'VP1' named entity 'FILM' named entity 'BK VIRUS' named entity 'FLUENCE' named entity 'DIAGNOSING' named entity 'GRAPHENE' named entity 'STAGE' named entity 'PICOSECOND' named entity 'LASER' named entity 'DEVICE' named entity 'MLG' named entity 'STRAIGHTFORWARD' named entity 'TO FORM' named entity 'VOLUME' named entity 'GRAPHENE' named entity 'DEVICE' named entity 'AMPLIFICATION' named entity 'STABLE' named entity 'INTEGRATION' named entity 'STABLE' named entity 'CYCLING' named entity 'UNIFORM' named entity 'FRAGMENTS' named entity 'PERFORMED BY' named entity 'ABLATION' named entity 'CYCLE' named entity 'CYCLING' named entity 'PRECISE' named entity 'BASED' named entity 'OUR' named entity 'HAS COST' named entity 'TEMPERATURE CONTROL' named entity 'PROCESS' named entity 'HEATER' named entity 'ELECTRODE' named entity 'AMPLIFIED' named entity 'OPTIMAL' named entity 'OVERLAP' named entity 'THERMAL' named entity 'DEMONSTRATED' named entity 'COST BENEFITS' named entity 'APPARATUS' named entity 'PICOSECOND' named entity 'REAGENT' named entity 'BCM' named entity 'RAPID' named entity 'ENHANCE' named entity 'RESULTS' named entity 'PROPOSED' named entity 'PERFORM'

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