About: Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality
Creator	Wahren, Britta Frieden, Miriam Koch, Troels Liang, Zicai Ljungberg, Karl Wahlestedt, Claes Westergaard, Majken Xu, Yunhe Elm E En, Joacim Kan Thonberg, Hå
topic	covid:09fc4c5e368a43d21f5130cb8474d61d65191dd9#this
source	Medline; PMC
abstract	Therapeutic application of the recently discovered small interfering RNA (siRNA) gene silencing phenomenon will be dependent on improvements in molecule bio-stability, specificity and delivery. To address these issues, we have systematically modified siRNA with the synthetic RNA-like high affinity nucleotide analogue, Locked Nucleic Acid (LNA). Here, we show that incorporation of LNA substantially enhances serum half-life of siRNA's, which is a key requirement for therapeutic use. Moreover, we provide evidence that LNA is compatible with the intracellular siRNA machinery and can be used to reduce undesired, sequence-related off-target effects. LNA-modified siRNAs targeting the emerging disease SARS, show improved efficiency over unmodified siRNA on certain RNA motifs. The results from this study emphasize LNA's promise in converting siRNA from a functional genomics technology to a therapeutic platform.
has issue date	2005-01-14(xsd:dateTime)
bibo:doi	10.1093/nar/gki193
bibo:pmid	15653644
has license	no-cc
sha1sum (hex)	09fc4c5e368a43d21f5130cb8474d61d65191dd9
schema:url	https://doi.org/10.1093/nar/gki193
resource representing a document's title	Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality
has PubMed Central identifier	PMC546170
has PubMed identifier	15653644
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:09fc4c5e368a43d21f5130cb8474d61d65191dd9#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/09fc4c5e368a43d21f5130cb8474d61d65191dd9
is schema:about of	named entity 'improvements' named entity 'intracellular' named entity 'technology' named entity 'silencing' named entity 'LNA' named entity 'EFFICIENCY' named entity 'PLATFORM' named entity 'HERE' named entity 'THERAPEUTIC USE' named entity 'KEY' named entity 'APPLICATION' named entity 'IMPROVED' named entity 'MACHINERY' named entity 'MOTIFS' named entity 'CERTAIN' named entity 'THERAPEUTIC' named entity 'DELIVERY' named entity '27S' named entity 'RECENTLY' named entity 'RESULTS' named entity 'MODIFIED' named entity 'TO REDUCE' named entity 'AFFINITY' named entity 'LIKE' named entity 'STABILITY' named entity 'FUNCTIONALITY' named entity 'SARS' named entity 'ADDRESS' named entity 'SYNTHETIC' covid:arg/09fc4c5e368a43d21f5130cb8474d61d65191dd9 named entity 'SIRNA' named entity 'LOCKED NUCLEIC ACID' named entity 'ANALOGUE' named entity 'MEDIATED' named entity 'REQUIREMENT' named entity 'STUDY' named entity 'DEPENDENT' named entity 'USED' named entity 'INTRACELLULAR' named entity 'ISSUES' named entity 'PROVIDE' named entity 'HAVE' named entity 'RELATED' named entity 'EVIDENCE' named entity 'GENE SILENCING' named entity 'SERUM' named entity 'CONVERTING' named entity 'FUNCTIONAL GENOMICS' named entity 'STABILITY' named entity 'SIRNAS' named entity 'SMALL INTERFERING RNA' named entity 'EFFECTS' named entity 'HIGH' named entity 'PHENOMENON' named entity 'PROMISE' named entity 'NUCLEOTIDE' named entity 'RNA' named entity 'HALF-LIFE' named entity 'SEQUENCE' named entity 'SIRNA' named entity 'LOCKED NUCLEIC ACID' named entity 'MOLECULE' named entity 'IS A' named entity 'LNA' named entity 'TARGETING' named entity 'TARGET' named entity 'COMPATIBLE WITH' named entity 'THESE' named entity 'DISEASE' named entity 'TECHNOLOGY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software