About: Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus

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About: Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus
Creator	Cong, Feng Huang, Ren Ma, Jingyun Guo, Pengju Zeng, Fanwen Huang, Bihong Ma, Lei
source	Elsevier; Medline; PMC
abstract	Abstract Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was associated with severe diarrhea disease in pigs. SADS-CoV was first detected and identified as the causative agent of a devastating swine disease outbreak in southern China in 2017. Routine monitoring and early detection of the source of infection is therefore integral to the prevention and control of SADS-CoV infection. In this study, a SYBR green-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique was established for rapid detection and monitoring of this emerging virus. Specific primers were designed based on the conserved region within the M gene of the viral genome. The lowest detection limit of the RT-qPCR assay was 10 copies/μL. This assay was specific and had no cross-reaction with other 11 swine viruses. The positive rate of 84 clinical samples for the SYBR green-based RT-qPCR and the conventional RT-PCR was 73.81% (62/84) and 53.57% (45/84), respectively. These results demonstrated that the SYBR green-based RT-qPCR technique was an effectively diagnostic method with higher sensitivity than probe-based RT-qPCR and gel-based RT-PCR for detection and epidemiological investigations of SADS-CoV.
has issue date	2019-03-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.12.010
bibo:pmid	30593837
has license	els-covid
sha1sum (hex)	0a512a4819c586666dfab93b1f5ee4fc02004494
schema:url	https://doi.org/10.1016/j.jviromet.2018.12.010
resource representing a document's title	Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus
has PubMed Central identifier	PMC7113735
has PubMed identifier	30593837
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:0a512a4819c586666dfab93b1f5ee4fc02004494#body_text
is schema:about of	named entity 'specific' named entity 'real-time' named entity 'swine' named entity 'SYBR' named entity 'coronavirus' named entity 'investigations' named entity 'emerging' named entity 'RT-qPCR' named entity 'SYBR' named entity 'DEVELOPMENT' named entity 'CROSS-REACTION' named entity 'INFECTION' named entity 'CAUSATIVE AGENT' named entity 'IS A' named entity 'DISEASE OUTBREAK' named entity 'CONSERVED REGION' named entity 'SENSITIVITY' named entity 'PREVENTION AND CONTROL' named entity 'PROBE' named entity 'DISEASE' named entity 'DETECTED' named entity 'TECHNIQUE' named entity 'SAMPLES' named entity 'VIRAL GENOME' named entity 'BCL' named entity 'SWINE' named entity 'DETECTION' named entity 'QPCR' named entity 'REAL-TIME RT-PCR' named entity 'SWINE ACUTE DIARRHEA SYNDROME CORONAVIRUS' named entity 'CLINICAL' named entity 'ESTABLISHED' named entity 'DETECTION LIMIT' named entity 'VIRUSES' named entity 'SEVERE DIARRHEA' named entity 'SYBR GREEN' named entity 'DIAGNOSTIC METHOD' named entity 'VIRUS' named entity 'AUTHORS' named entity 'ASSOCIATED WITH' named entity 'THESE' named entity 'NOVEL CORONAVIRUS' named entity 'SPECIFIC' named entity 'SYBR GREEN' named entity 'STUDY' named entity 'RT-PCR' named entity 'CONVENTIONAL' named entity 'EARLY DETECTION' named entity 'RAPID' named entity 'RT-PCR ASSAY' named entity 'BASED' named entity 'MONITORING' named entity 'M GENE' named entity 'IDENTIFIED' named entity 'ROUTINE' named entity 'RT-QPCR' named entity 'LOWEST' named entity 'SWINE ACUTE DIARRHEA SYNDROME CORONAVIRUS' named entity 'SWINE DISEASE' named entity 'EPIDEMIOLOGICAL' named entity 'CHINA' named entity 'SOUTHERN' named entity 'RESULTS' named entity 'ASSAY' named entity 'BASED' named entity 'DETECTION' named entity 'REVERSE TRANSCRIPTION QUANTITATIVE POLYMERASE CHAIN REACTION' named entity 'PIGS' named entity 'GEL' named entity 'HIGHER' named entity 'POSITIVE RATE' named entity 'INTEGRAL' named entity 'COPIES' named entity 'PRIMERS' named entity 'REAL-TIME'

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