About: Automated cell-based luminescence assay for profiling antiviral compound activity against enteroviruses

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About: Automated cell-based luminescence assay for profiling antiviral compound activity against enteroviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Automated cell-based luminescence assay for profiling antiviral compound activity against enteroviruses
Creator	Wang, Yan Zhang, Yong Lv, Wanyu Zhang, Mingyu Zhang, Yanyang
source	Medline; PMC
abstract	We describe the development, optimisation, and validation of an automated, cell-based and high-throughput screening assay using existing luminescence-based ATPlite reagents for identifying antiviral compounds that inhibit enterovirus replication. Antiviral efficacy was determined by measuring the ATP levels in cells that were protected from the viral cytopathic effect (CPE) by the antiviral compounds pleconaril and rupintrivir. CPE-based assay conditions were optimised at a cell density of 5000 cells/well and a viral infection dose of 100 CCID(50) in 384-well plates. The assay exhibited excellent robustness, with Z′-factor values between 0.75 and 0.82, coefficients of variation between 0.33% and 1.45%, and signal-to-background ratios ranging from 6.92 to 22.6 when testing three enterovirus A71 isolates circulating in China. The assay was also suitable for screening other picornaviruses, such as poliovirus, coxsackievirus, echovirus, and parechovirus.
has issue date	2019-04-15(xsd:dateTime)
bibo:doi	10.1038/s41598-019-42160-7
bibo:pmid	30988314
has license	cc-by
sha1sum (hex)	0abbc904e7a1b2e1c3ea7be75ee2a61931e0a941
schema:url	https://doi.org/10.1038/s41598-019-42160-7
resource representing a document's title	Automated cell-based luminescence assay for profiling antiviral compound activity against enteroviruses
has PubMed Central identifier	PMC6465263
has PubMed identifier	30988314
schema:publication	Sci Rep
resource representing a document's body	covid:0abbc904e7a1b2e1c3ea7be75ee2a61931e0a941#body_text
is schema:about of	named entity '2C CELL' named entity 'PLECONARIL' named entity 'FACTOR' named entity 'CHINA' named entity 'OTHER PICORNAVIRUSES' named entity '0.82' named entity 'VALIDATION' named entity 'COXSACKIEVIRUS' named entity 'CIRCULATING' named entity 'RATIOS' named entity 'excellent' named entity 'robustness' named entity 'ACTIVITY' named entity 'ATP' named entity 'DETERMINED BY' named entity 'CPE' named entity '1.45' named entity 'CYTOPATHIC EFFECT' named entity 'USING' named entity 'ENTEROVIRUS A71' named entity 'AUTOMATED' named entity 'ENTEROVIRUS' named entity 'EXCELLENT' named entity '100' named entity '384' named entity 'PARECHOVIRUS' named entity 'ASSAY' named entity '80%' named entity 'CONDITIONS' named entity 'ISOLATES' named entity 'VIRAL INFECTION' named entity 'VARIATION' named entity 'CELLS' named entity 'LUMINESCENCE' named entity 'LEVELS' named entity 'VIRAL' named entity 'IDENTIFYING' named entity 'VALUES' named entity '5000' named entity 'AUTOMATED' named entity 'ENTEROVIRUSES' named entity 'ASSAY' named entity 'BASED' named entity 'ANTIVIRAL' named entity 'CELL' named entity 'ANTIVIRAL' named entity 'BASED' named entity 'PLATES' named entity 'DESCRIBE' named entity 'INFECTION DOSE' named entity 'SIGNAL' named entity '0.75' named entity 'A CELL' named entity 'REPLICATION' named entity 'RUPINTRIVIR' named entity 'BACKGROUND' named entity 'SCREENING' named entity 'CCID' named entity '0.33' named entity 'LUMINESCENCE' named entity 'COMPOUND' named entity 'TESTING' named entity 'REAGENTS' named entity 'SUITABLE' named entity 'MEASURING' named entity 'DEVELOPMENT' named entity 'CELL DENSITY' named entity 'POLIOVIRUS' named entity 'ECHOVIRUS' named entity 'EFFICACY' covid:arg/0abbc904e7a1b2e1c3ea7be75ee2a61931e0a941 named entity 'efficacy' named entity 'Antiviral' named entity 'conditions' named entity 'existing' named entity 'echovirus' named entity 'A71'

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