About: Importance of coronavirus negative-strand genomic RNA synthesis prior to subgenomic RNA transcription

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Importance of coronavirus negative-strand genomic RNA synthesis prior to subgenomic RNA transcription
Creator	Makino, Shinji An, Sungwhan Maeda, Akihiko
topic	covid:0adc67dd5357d6059c21d54281273e4ffa20f6c4#this
Source	Elsevier; Medline; PMC
abstract	Abstract The (−)-strand viral RNAs that result from after infection of cells with coronaviruses, which possess RNA genomes of message polarity, are genomic-sized and subgenomic-sized. Each of the (−)-strand subgenomic RNAs corresponds in size to each of the subgenomic mRNA species that are made in infected cells. We tested whether (−)-strand subgenomic RNAs might initially be synthesized from the input single-stranded (+)-strand genomic RNA prior to the production of subgenomic mRNAs. We used a mouse hepatitis virus (MHV) defective interfering (DI) RNA, from which subgenomic RNA was produced in DI RNA-replicating cells, because this DI RNA had a functional MHV intergenic region inserted in its interior. MHV samples containing the DI particles were irradiated with UV-light and then superinfected into cells that had been infected with MHV 4 h prior to superinfection. Northern blot analysis of intracellular RNAs that were extracted 3 h after superinfection showed that genomic DI RNA and subgenomic DI RNA had similar UV-target sizes, indicating that (−)-strand genomic DI RNA synthesis from input genomic DI RNA probably occurred prior to the subgenomic-size DI RNA synthesis. We discuss why, in the course of coronavirus transcription, (−)-strand genomic-length coronavirus RNA synthesis might occur before subgenomic-sized RNAs of either polarity are made.
has issue date	1998-09-30(xsd:dateTime)
bibo:doi	10.1016/s0168-1702(98)00090-2
bibo:pmid	9833884
has license	els-covid
sha1sum (hex)	0adc67dd5357d6059c21d54281273e4ffa20f6c4
schema:url	https://doi.org/10.1016/s0168-1702%2898%2900090-2
resource representing a document's title	Importance of coronavirus negative-strand genomic RNA synthesis prior to subgenomic RNA transcription
has PubMed Central identifier	PMC7127783
has PubMed identifier	9833884
schema:publication	Virus Research
resource representing a document's body	covid:0adc67dd5357d6059c21d54281273e4ffa20f6c4#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/0adc67dd5357d6059c21d54281273e4ffa20f6c4
is schema:about of	named entity 'cells' named entity 'production' named entity 'RNA' named entity 'species' named entity 'functional' named entity 'RNA' named entity 'genomic' covid:arg/0adc67dd5357d6059c21d54281273e4ffa20f6c4 named entity 'MHV' named entity 'genomic' named entity 'infected' named entity 'genomic' named entity 'produced' named entity 'MHV' named entity 'possess' named entity 'RNAs' named entity 'RNA' named entity 'subgenomic' named entity 'coronaviruses' named entity 'mRNAs' named entity 'MHV' named entity 'subgenomic' named entity 'intracellular' named entity 'coronavirus' named entity 'subgenomic mRNA' named entity 'subgenomic RNA' named entity 'superinfection' named entity 'subgenomic RNA' named entity 'negative-strand' named entity 'RNAs' named entity 'RNA' named entity 'Intracellular' named entity 'transcription' named entity 'RNA' named entity 'RNAs' named entity 'RNA replication' named entity 'subgenomic' named entity 'chemiluminescent' named entity 'lipofection' named entity 'control group' named entity 'RNA' named entity 'RNA' named entity 'control groups' named entity 'hybridization' named entity 'mRNAs' named entity 'RNA' named entity 'subgenomic' named entity 'double-stranded' named entity 'MHV' named entity 'RNA' named entity 'RNA' named entity 'RNAs' named entity 'RNA' named entity 'RNA' named entity 'digoxigenin' named entity 'X-ray' named entity 'Northern blot analysis' named entity 'RNAs' named entity 'infection' named entity 'double-stranded' named entity 'transcription' named entity 'subgenomic' named entity 'RNA synthesis' named entity 'MHV' named entity 'MHV' named entity 'subgenomic RNA' named entity 'alkaline phosphatase' named entity 'subgenomic mRNA' named entity 'coronavirus' named entity 'subgenomic' named entity 'primers' named entity 'Northern blot analysis' named entity 'subgenomic' named entity 'RNA replication' named entity 'RNAs' named entity 'RNA' named entity 'Virus'

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