About: A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein

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About: A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein
Creator	Wang, Lin-Fa Fukushi, Shuetsu Bazartseren, Boldbaatar Hotta, Kozue Kaku, Yoshihiro Marsh, Glenn Inoue, Satoshi Noguchi, Akira Okutani, Akiko Yamada, Akio Broder, Christopher Mceachern, Jennifer
source	Elsevier; Medline; PMC
abstract	Abstract Nipah virus (NiV) is a new zoonotic paramyxovirus that emerged in 1998 and is now classified in the genus Henipavirus along with the closely related Hendra virus (HeV). NiV is highly pathogenic in several vertebrate species including humans, and the lack of available vaccines or specific treatment restricts it to biosafety level 4 (BSL4) containment. A serum neutralization test was developed for measuring NiV neutralizing antibodies under BSL2 conditions using a recombinant vesicular stomatitis virus (VSV) expressing green fluorescent protein (GFP) and bearing the F and G proteins of NiV (VSV–NiV–GFP). The neutralization titers were obtained by counting GFP-expressing cells or by measuring fluorescence. The performance of this new assay was compared against the conventional test using live NiV with panels of sera from several mammalian species, including sera from NiV outbreaks, experimental infections, as well as HeV-specific sera. The results obtained with the VSV–NiV–GFP based test correlated with those obtained using live NiV. Using a 50% reduction in VSV–NiV–GFP infected cells as the cut-off for neutralization, this new assay demonstrated its potential as an effective tool for detecting NiV neutralizing antibodies under BSL2 containment with greater speed, sensitivity and safety as compared to the conventional NiV serum neutralization test.
has issue date	2009-09-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2009.04.037
bibo:pmid	19433112
has license	els-covid
sha1sum (hex)	0b028bc596add636668e7b46cf681d1011a4139e
schema:url	https://doi.org/10.1016/j.jviromet.2009.04.037
resource representing a document's title	A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein
has PubMed Central identifier	PMC7112920
has PubMed identifier	19433112
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:0b028bc596add636668e7b46cf681d1011a4139e#body_text
is schema:about of	covid:arg/0b028bc596add636668e7b46cf681d1011a4139e named entity 'NiV' named entity 'assay' named entity 'neutralizing antibodies' named entity 'Hendra virus' named entity 'NiV' named entity 'vesicular stomatitis virus' named entity 'GFP' named entity 'VSV' named entity 'paramyxovirus' named entity 'neutralization test' named entity 'biosafety level 4' named entity 'serum' named entity 'assay' named entity 'vaccines' named entity 'GFP' named entity 'Nipah virus' named entity 'neutralization test' named entity 'antibodies' named entity 'high-throughput' named entity 'Vero cell' named entity 'fruit bat' named entity 'cDNA' named entity 'antibody' named entity 'pig' named entity 'sero' named entity 'VSV' named entity 'gene' named entity 'GFP' named entity 'neutralization test' named entity 'VSV' named entity 'GFP' named entity 'VSV' named entity 'NiV' named entity 'severe acute respiratory syndrome coronavirus' named entity 'toxicity' named entity 'VSV' named entity 'Indonesia' named entity 'viruses' named entity 'VSV' named entity 'VSV' named entity 'BSL2' named entity 'neutralization test' named entity 'MOI' named entity 'immunization' named entity 'gene' named entity 'hantavirus' named entity 'NiV' named entity 'titer' named entity 'pseudotyped viruses' named entity 'OIE' named entity 'prophylactic measures' named entity 'World Organization for Animal Health' named entity '24 hrs' named entity 'EMEM' named entity 'serum' named entity 'antibodies' named entity 'GFP' named entity 'GFP' named entity 'vector' named entity 'transfected' named entity 'neutralization test' named entity 'gene' named entity 'Malaysia' named entity 'neutralization test' named entity 'Vero cells' named entity 'neutralization tests' named entity 'cross-reactivity' named entity 'GFP' named entity 'NiV'

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