About: A Recombinant Fungal Lectin for Labeling Truncated Glycans on Human Cancer Cells

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About: A Recombinant Fungal Lectin for Labeling Truncated Glycans on Human Cancer Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Recombinant Fungal Lectin for Labeling Truncated Glycans on Human Cancer Cells
Creator	Le Pendu, Jacques Imberty, Anne Audfray, Aymeric Beldjoudi, Mona Boos, Irene Bouras, Mourad Breiman, Adrien Busser, Benoit Coll, Jean-Luc Hurbin, Amandine Lantuejoul, Sylvie Unverzagt, Carlo Varrot, Annabelle
source	Medline; PMC
abstract	Cell surface glycoconjugates present alterations of their structures in chronic diseases and distinct oligosaccharide epitopes have been associated with cancer. Among them, truncated glycans present terminal non-reducing β-N-acetylglucosamine (GlcNAc) residues that are rare on healthy tissues. Lectins from unconventional sources such as fungi or algi provide novel markers that bind specifically to such epitopes, but their availability may be challenging. A GlcNAc-binding lectin from the fruiting body of the fungus Psathyrella velutina (PVL) has been produced in good yield in bacterial culture. A strong specificity for terminal GlcNAc residues was evidenced by glycan array. Affinity values obtained by microcalorimetry and surface plasmon resonance demonstrated a micromolar affinity for GlcNAcβ1-3Gal epitopes and for biantennary N-glycans with GlcNAcβ1-2Man capped branches. Crystal structure of PVL complexed with GlcNAcβ1-3Gal established the structural basis of the specificity. Labeling of several types of cancer cells and use of inhibitors of glycan metabolism indicated that rPVL binds to terminal GlcNAc but also to sialic acid (Neu5Ac). Analysis of glycosyltransferase expression confirmed the higher amount of GlcNAc present on cancer cells. rPVL binding is specific to cancer tissue and weak or no labeling is observed for healthy ones, except for stomach glands that present unique αGlcNAc-presenting mucins. In lung, breast and colon carcinomas, a clear delineation could be observed between cancer regions and surrounding healthy tissues. PVL is therefore a useful tool for labeling agalacto-glycans in cancer or other diseases.
has issue date	2015-06-04(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0128190
bibo:pmid	26042789
has license	cc-by
sha1sum (hex)	0bca841af11fa978a07f916ec43b34d7c88b71c1
schema:url	https://doi.org/10.1371/journal.pone.0128190
resource representing a document's title	A Recombinant Fungal Lectin for Labeling Truncated Glycans on Human Cancer Cells
has PubMed Central identifier	PMC4456360
has PubMed identifier	26042789
schema:publication	PLoS One
resource representing a document's body	covid:0bca841af11fa978a07f916ec43b34d7c88b71c1#body_text
is schema:about of	named entity 'basis' named entity 'GlcNAc' named entity 'bacterial culture' named entity 'glycoconjugates' named entity 'GlcNAc' named entity 'Labeling' named entity 'GlcNAc' named entity 'Psathyrella' named entity 'binds' named entity 'N-glycans' named entity 'Cancer' named entity 'Recombinant' covid:arg/0bca841af11fa978a07f916ec43b34d7c88b71c1 named entity 'cancer' named entity 'bind' named entity 'carcinomas' named entity 'lectin' named entity 'metabolism' named entity 'cancer' named entity 'lung' named entity 'cells' named entity 'GlcNAc' named entity 'bacterial culture' named entity 'velutina' named entity 'non-reducing' named entity 'surface plasmon resonance' named entity 'epitopes' named entity 'fungi' named entity 'epitopes' named entity 'glycans' named entity 'fungus' named entity 'Psathyrella' named entity 'microcalorimetry' named entity 'capped' named entity 'stomach' named entity '2.2' named entity 'lectin' named entity 'transferases' named entity 'GlcNAc' named entity 'sialidase' named entity 'electron density' named entity 'confocal microscope' named entity 'higher concentration' named entity 'GE Healthcare' named entity 'lectin' named entity 'ligands' named entity 'staining' named entity 'protein' named entity 'Inserm' named entity 'N-glycans' named entity 'cell line' named entity 'PBS' named entity 'GlcNAc' named entity 'amine' named entity 'oligosaccharide' named entity 'disaccharide' named entity 'colon carcinomas' named entity 'p-values' named entity 'ampicillin' named entity 'A280' named entity 'hormone' named entity 'Ethanol' named entity 'Neu5Ac' named entity 'hexosaminidase' named entity 'PBS' named entity 'lectin' named entity 'glycan' named entity 'lectin' named entity 'w/v'

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