About: Diagnóstico de virus respiratorios utilizando un sistema automatizado de PCR múltiples (FilmArray) y su comparación con métodos convencionales

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About: Diagnóstico de virus respiratorios utilizando un sistema automatizado de PCR múltiples (FilmArray) y su comparación con métodos convencionales Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diagnóstico de virus respiratorios utilizando un sistema automatizado de PCR múltiples (FilmArray) y su comparación con métodos convencionales
Creator	Carballal, Guadalupe Echavarria, Marcela Ricarte, Carmen Marcone, Débora
Source	Elsevier; Medline; PMC
abstract	Acute respiratory infections, which are commonly caused by viruses, are an important cause of morbidity and mortality in children. In Argentina, national surveillance programs for the detection of respiratory viruses are usually performed by using immunofluorescence (IF) assays, although it is well known that molecular methods are more sensitive. An automated multiplex PCR device, the FilmArray-Respiratory Panel (FilmArray-RP), can detect 17 viral and 3 bacterial pathogens in a closed system that requires only 5 min of hands-on time and 1 h of instrumentation time. A total of 315 respiratory samples from children under 6 years of age suffering from acute respiratory infections, were studied by IF for 8 respiratory viruses and by RT-PCR for rhinoviruses. Later, these samples were tested by the FilmArray-RP. The positivity frequency obtained for the 9 viruses tested was 75 % by IF/RT-PCR and 92 % by the FilmArray-RP. The positive and negative percent agreement between both methods was 70.5 % whereas the negative percent agreement was 99.6 % (95 % confidence interval:65.5-75.1 and 99.2-99.8 respectively). The FilmArray-RP allowed a higher positive diagnosis (97 %) and detected other viruses such as coronavirus NL63, 229E, OC43, HKU1 (10 %) and bocavirus (18 %). In addition, this method identified multiple coinfections (39 %) with 2, 3, 4 and up to 5 different viruses. At present, IF is still the most frequently used method in most Latin American countries for respiratory viruses diagnosis due to its low cost, its capability to process a high number of samples simultaneously and the fast determination of results for the most frequent viruses, which are available within 5 h. However, the coming incorporation of molecular methods in routine procedures will significantly increase the diagnostic yield of these infections.
has issue date	2015-02-28(xsd:dateTime)
bibo:doi	10.1016/j.ram.2014.12.003
bibo:pmid	25735216
has license	no-cc
sha1sum (hex)	0bdca750e8aa93db739f513bcd1eddd854380d2e
schema:url	https://doi.org/10.1016/j.ram.2014.12.003
resource representing a document's title	Diagnóstico de virus respiratorios utilizando un sistema automatizado de PCR múltiples (FilmArray) y su comparación con métodos convencionales
has PubMed Central identifier	PMC7131815
has PubMed identifier	25735216
schema:publication	Rev Argent Microbiol
resource representing a document's body	covid:0bdca750e8aa93db739f513bcd1eddd854380d2e#body_text
is schema:about of	named entity '12 DE OCTUBRE' named entity 'sistema' named entity 'PCR' named entity '2015' named entity 'VIRUS' named entity 'C R' named entity 'INTERNET' named entity '2014' named entity 'seguida' named entity '2014' named entity 'PCR' named entity 'CDC' named entity 'RT-PCR' named entity 'el presente' named entity 'concordancia' named entity 'tabla' named entity 'la Ley' named entity 'Stata' named entity 'Argentina' named entity 'influenza' named entity 'el 18' named entity 'Helsinki' named entity 'Argentina' named entity 'enterovirus' named entity 'influenza' named entity 'hace' named entity 'AdV' named entity 'PCR' named entity 'AdV' named entity 'el alto' named entity 'HCoV-HKU1' named entity 'PCR' named entity 'Habeas Data' named entity 'HCoV-229E' named entity 'RT-PCR' named entity 'B. pertussis' named entity 'HCoV-OC43' named entity 'HCoV-NL63' named entity 'ambas' named entity 'cartucho' named entity 'RT-PCR' named entity 'informa' named entity 'mismo' named entity 'RT-PCR' named entity 'PICT' named entity 'PCR' named entity 'STATA' named entity 'cerrado' named entity 'usando' named entity 'cartucho' named entity 'tabla' named entity 'PCR' named entity 'Helsinki' named entity 'AdV' named entity 'C. pneumoniae' named entity 'RT-PCR' named entity 'AdV' named entity 'tabla' named entity 'RT-PCR' named entity '11.1' named entity 'FDA' named entity 'en 3' named entity 'PCR' named entity 'el 12' named entity 'virus' named entity 'PCR' named entity 'Internet' named entity '12 de octubre' named entity '2015' named entity 'Internet' named entity 'virus' named entity 'PCR'
is part of of	Diagnóstico de virus respiratorios utilizando un sistema automatizado de PCR múltiples (FilmArray) y su comparación con métodos convencionales Acute respiratory infections, which are commonly caused by viruses, are an important cause of morbidity and mortality in children. In Argentina, national surveillance programs for the detection of respiratory viruses are usually performed by using immunofluorescence (IF) assays, although it is well known that molecular methods are more sensitive. An automated multiplex PCR device, the FilmArray-Respiratory Panel (FilmArray-RP), can detect 17 viral and 3 bacterial pathogens in a closed system that requires only 5 min of hands-on time and 1 h of instrumentation time. A total of 315 respiratory samples from children under 6 years of age suffering from acute respiratory infections, were studied by IF for 8 respiratory viruses and by RT-PCR for rhinoviruses. Later, these samples were tested by the FilmArray-RP. The positivity frequency obtained for the 9 viruses tested was 75 % by IF/RT-PCR and 92 % by the FilmArray-RP. The positive and negative percent agreement between both methods was 70.5 % whereas the negative percent agreement was 99.6 % (95 % confidence interval:65.5-75.1 and 99.2-99.8 respectively). The FilmArray-RP allowed a higher positive diagnosis (97 %) and detected other viruses such as coronavirus NL63, 229E, OC43, HKU1 (10 %) and bocavirus (18 %). In addition, this method identified multiple coinfections (39 %) with 2, 3, 4 and up to 5 different viruses. At present, IF is still the most frequently used method in most Latin American countries for respiratory viruses diagnosis due to its low cost, its capability to process a high number of samples simultaneously and the fast determination of results for the most frequent viruses, which are available within 5 h. However, the coming incorporation of molecular methods in routine procedures will significantly increase the diagnostic yield of these infections. covid:0bdca750e8aa93db739f513bcd1eddd854380d2e#body_text

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