About: Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

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About: Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
Creator	Komatsuzawa, Hitoshi Kitagawa, Masae Kurihara, Hidemi Nagamine, Kentaro Ogawa, Ikuko Oka, Hiroko Ouhara, Kazuhisa
source	Elsevier; Medline; PMC
abstract	This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.
has issue date	2020-06-25(xsd:dateTime)
bibo:doi	10.1016/j.ab.2020.113812
bibo:pmid	32592714
has license	no-cc
sha1sum (hex)	0be2523b4549e32afac4bac0ef24f1d89822d9f2
schema:url	https://doi.org/10.1016/j.ab.2020.113812
resource representing a document's title	Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification
has PubMed Central identifier	PMC7315161
has PubMed identifier	32592714
schema:publication	Anal Biochem
resource representing a document's body	covid:0be2523b4549e32afac4bac0ef24f1d89822d9f2#body_text
is schema:about of	named entity 'loop-mediated isothermal amplification' named entity 'cnm' named entity 'gene' named entity 'cnm' named entity 'viruses' named entity 'Hiroshima University' named entity 'calcein' named entity 'ion' named entity 'Mitis' named entity 'cerebral microbleeds' named entity 'milliliter' named entity 'Saliva' named entity 'Hiroshima University' named entity 'Candida spp' named entity 'S. mutans' named entity 'brain heart infusion broth' named entity 'saliva' named entity 'Japan' named entity 'loop-mediated isothermal amplification' named entity 'S. mutans' named entity 'cnm' named entity 'hemorrhagic stroke' named entity 'bacteria' named entity 'periodontal disease' named entity 'pathogen' named entity 'human genomic' named entity 'microhemorrhage' named entity 'fluorescence' named entity 'cnm' named entity 'manganese' named entity 'tooth loss' named entity 'cnm' named entity 'risk factor' named entity 'plasmid vector' named entity 'thymidine' named entity 'cerebral microbleeds' named entity 'cloning vector' named entity 'cnm' named entity '1, 2' named entity 'Streptococcus mutans' named entity 'cnm' named entity 'bacteria' named entity 'S. mutans' named entity 'gene' named entity 'systemic diseases' named entity 'cnm' named entity 'S. mutans' named entity 'gene' named entity 'gene' named entity 'mycoplasma' named entity 'plasmid vector' named entity 'saliva' named entity 'P. gingivalis' named entity 'cnm' named entity 'caries' named entity 'bacterial colonies' named entity 'cnm' named entity 'infection' named entity 'influenza' named entity 'cnm' named entity 'genomic DNA' named entity 'fluorescence' named entity 'S. mutans' named entity 'cnm' named entity 'cnm' named entity 'Specificity and sensitivity' named entity 'epidemiological research' named entity 'cnm' named entity 'S. mutans' named entity 'saliva' named entity 'sensitivity test' named entity 'S. mutans' named entity 'gene' named entity 'Promega' named entity 'ion'

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