About: Generation and characterization of anti-α-enolase single-chain antibodies in chicken

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About: Generation and characterization of anti-α-enolase single-chain antibodies in chicken Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation and characterization of anti-α-enolase single-chain antibodies in chicken
Creator	Lee, Yu-Ching Leu, Sy-Jye Yang, Yi-Yuan Huang, I-Jen Huang, Shih-Yi Liu, Ko-Jiunn Lu, Hsu-Feng Shih, Neng-Yao
topic	covid:0bf614ec42d27fa30f001e67ee0c2ae40fe8ed3b#this
Source	Elsevier; Medline; PMC
abstract	It was previously reported that up-regulation of α-enolase protein was detected in 65% of patients with non-small cell lung cancers (NSCLC). Moreover, a high titer of anti-α-enolase antibodies was developed in a smaller proportion (7.4%) of these patients than in non-tumor-associated patients and healthy subjects. In the present study, we characterized polyclonal and single-chain variable fragment (scFv) anti-α-enolase antibodies from immunized chickens. The E. coli-derived recombinant α-enolase protein was purified to its high homogenicity as verified by SDS-PAGE. After the 4th immunization, a high titer of specific polyclonal anti-α-enolase antibodies was elicited in immunized chickens and specifically recognized the purified human α-enolase antigen as determined by Western blot and ELISA. The expressed heavy and light chain variable genes (VH and VL) were isolated from spleen B cells and amplified to construct phage antibody libraries containing scFv molecules. After four rounds of panning selection, the scFv antibodies of randomly chosen clones were expressed and their binding specificity to α-enolase protein was verified using competitive ELISA, flow cytometry and immunofluorescence staining. Nucleotide sequence analysis from 10 α-enolase binding clones showed that 3 (30%) clones used identical heavy and light genes for scFv antibody expression, as represented by EnL5. Notably, amino acid changes in complementarity-determining regions (CDRs) were more frequently observed than those in framework regions (FRs) in all clones, indicating a strong affinity selection through mutations. All together, it is believed that these polyclonal and scFv IgY antibodies may be helpful in the development of molecular diagnostic and therapeutic agents for lung cancers in the future.
has issue date	2010-10-15(xsd:dateTime)
bibo:doi	10.1016/j.vetimm.2010.06.001
bibo:pmid	20655599
has license	no-cc
sha1sum (hex)	0bf614ec42d27fa30f001e67ee0c2ae40fe8ed3b
schema:url	https://doi.org/10.1016/j.vetimm.2010.06.001
resource representing a document's title	Generation and characterization of anti-α-enolase single-chain antibodies in chicken
has PubMed Central identifier	PMC7112641
has PubMed identifier	20655599
schema:publication	Vet Immunol Immunopathol
resource representing a document's body	covid:0bf614ec42d27fa30f001e67ee0c2ae40fe8ed3b#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/0bf614ec42d27fa30f001e67ee0c2ae40fe8ed3b
is schema:about of	named entity 'ELISA' named entity 'THEIR' named entity 'BINDING' named entity 'HELPFUL' named entity 'SCFV ANTIBODY' named entity 'DIAGNOSTIC' named entity 'IMMUNOFLUORESCENCE STAINING' named entity 'DEVELOPMENT' named entity 'LIGHT CHAIN' named entity 'STRONG' named entity 'CHICKENS' named entity 'EXPRESSED' named entity 'PATIENTS' named entity 'FREQUENTLY' named entity 'NON-SMALL CELL' named entity 'PRESENT' named entity 'FLOW CYTOMETRY' named entity 'PREVIOUSLY' named entity 'E. COLI' named entity 'LUNG CANCERS' named entity 'ANTIBODIES' named entity 'TITER' named entity 'HEAVY' named entity 'HIGH' named entity 'PHAGE' named entity 'HEALTHY SUBJECTS' named entity 'IMMUNIZED' named entity 'LIGHT' named entity 'EXPRESSION' named entity 'developed' named entity 'patients' named entity 'clones' named entity 'selection' named entity 'CDRs' named entity 'binding' named entity 'development' named entity 'strong' named entity 'variable' named entity 'light' named entity 'libraries' named entity 'antibodies' named entity 'ENOLASE' named entity 'INDICATING' named entity 'IGY' named entity 'PANNING' named entity 'GENES' named entity 'SPLEEN' named entity 'WESTERN BLOT' named entity '4TH' named entity 'SINGLE-CHAIN VARIABLE FRAGMENT' named entity 'AFFINITY SELECTION' named entity 'CONSTRUCT' named entity 'HUMAN' named entity 'CHICKEN' covid:arg/0bf614ec42d27fa30f001e67ee0c2ae40fe8ed3b named entity 'OBSERVED' named entity 'TUMOR' named entity 'FUTURE' named entity 'USED' named entity 'DETERMINED BY' named entity 'VERIFIED' named entity 'NUCLEOTIDE SEQUENCE' named entity 'CONTAINING' named entity 'PURIFIED' named entity 'MAY BE' named entity 'CHANGES' named entity 'LIBRARIES' named entity 'ENOLASE' named entity '287' named entity 'ELICITED' named entity 'IDENTICAL' named entity 'AMINO ACID'

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