About: A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry
Creator	Ippolito, Giuseppe Horejsh, Douglas Martini, Federico Poccia, Fabrizio Capobianchi, Maria Caro, Antonino
Source	Medline; PMC
abstract	Molecular beacons are dual-labelled probes that are typically used in real-time PCR assays, but have also been conjugated with solid matrices for use in microarrays or biosensors. We have developed a fluid array system using microsphere-conjugated molecular beacons and the flow cytometer for the specific, multiplexed detection of unlabelled nucleic acids in solution. For this array system, molecular beacons were conjugated with microspheres using a biotin-streptavidin linkage. A bridged conjugation method using streptavidin increased the signal-to-noise ratio, allowing for further discrimination of target quantitation. Using beads of different sizes and molecular beacons in two fluorophore colours, synthetic nucleic acid control sequences were specifically detected for three respiratory pathogens, including the SARS coronavirus in proof-of-concept experiments. Considering that routine flow cytometers are able to detect up to four fluorescent channels, this novel assay may allow for the specific multiplex detection of a nucleic acid panel in a single tube.
has issue date	2005-01-19(xsd:dateTime)
bibo:doi	10.1093/nar/gni015
bibo:pmid	15659574
has license	no-cc
sha1sum (hex)	0c3220694f8a18b6dcd64afae28c4784190e4ee6
schema:url	https://doi.org/10.1093/nar/gni015
resource representing a document's title	A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry
has PubMed Central identifier	PMC548373
has PubMed identifier	15659574
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:0c3220694f8a18b6dcd64afae28c4784190e4ee6#body_text
is schema:about of	named entity 'molecular beacons' named entity 'ABLE TO' named entity 'MOLECULAR BEACON' named entity 'BEADS' named entity 'ALLOW' named entity 'BIOSENSORS' named entity 'FLOW' named entity 'DIFFERENT' covid:arg/0c3220694f8a18b6dcd64afae28c4784190e4ee6 named entity 'PANEL' named entity 'NUCLEIC ACID' named entity 'DETECTION' named entity 'TUBE' named entity 'SINGLE' named entity 'USE' named entity 'SYNTHETIC NUCLEIC ACID' named entity 'MICROSPHERES' named entity 'BIOTIN' named entity 'NUCLEIC ACIDS' named entity 'STREPTAVIDIN' named entity 'PROBES' named entity 'CONCEPT' named entity 'BUT' named entity 'SYSTEM' named entity 'FLOW CYTOMETRY' named entity 'SARS CORONAVIRUS' named entity 'INCREASED' named entity 'DETECTION' named entity 'BEAD' named entity 'METHOD' named entity 'LINKAGE' named entity 'DISCRIMINATION' named entity 'EXPERIMENTS' named entity 'CHANNELS' named entity 'NUCLEIC ACIDS' named entity 'QUANTITATION' named entity 'PROOF' named entity 'SOLID' named entity 'REAL-TIME PCR ' named entity 'CONJUGATION' named entity 'ALLOWING' named entity 'ARRAY' named entity 'MULTIPLEX' named entity 'MICROARRAYS' named entity 'SIZES' named entity 'CONSIDERING' named entity 'RATIO' named entity 'FLUORESCENT' named entity 'FLUID' named entity 'TARGET' named entity 'COLOURS' named entity 'NOVEL' named entity 'MOLECULAR' named entity 'MICROSPHERE' named entity 'DUAL' named entity 'SPECIFIC' named entity 'HAVE' named entity 'FLOW CYTOMETER' named entity 'USING' named entity 'OF-' named entity 'RESPIRATORY PATHOGENS' named entity 'FLUOROPHORE' named entity 'MATRICES' named entity 'CONJUGATED' named entity 'ROUTINE' named entity 'ASSAY' named entity 'BASED' named entity 'TO DETECT' named entity 'INCLUDING' named entity 'SOLUTION' named entity 'USED' named entity 'DETECTED' named entity 'ASSAY' named entity 'UP TO' named entity 'CONTROL SEQUENCES' named entity 'SIGNAL'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software