About: Amplification refractory mutation system PCR assays for the detection of variola and Orthopoxvirus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Amplification refractory mutation system PCR assays for the detection of variola and Orthopoxvirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Amplification refractory mutation system PCR assays for the detection of variola and Orthopoxvirus
Creator	Meyer, Hermann Brightwell, Gale Damon, Inger Kline, Richard Pulford, David Ulaeto, David
topic	covid:0c91f2c73d89deadccd0db5359eb1c95242ac027#this
Source	Elsevier; Medline; PMC
abstract	PCR assays that can identify the presence of variola virus (VARV) sequences in an unknown DNA sample were developed using principles established for the amplification refractory mutation system (ARMS). The assay’s specificity utilised unique single nucleotide polymorphisms (SNP) identified among Orthopoxvirus (OPV) orthologs of the vaccinia virus Copenhagen strain A13L and A36R genes. When a variola virus specific primer was used with a consensus primer in an ARMS assay with different Orthopoxvirus genomes, a PCR product was only amplified from variola virus DNA. Incorporating a second consensus primer into the assay produced a multiplex PCR that provided Orthopoxvirus generic and variola-specific products with variola virus DNA. We tested two single nucleotide polymorphisms with a panel of 43 variola virus strains, collected over 40 years from countries across the world, and have shown that they provide reliable markers for variola virus identification. The variola virus specific primers did not produce amplicons with either assay format when tested with 50 other Orthopoxvirus DNA samples. Our analysis shows that these two polymorphisms were conserved in variola virus genomes and provide a reliable signature of Orthopoxvirus species identification.
has issue date	2004-02-13(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2004.01.001
bibo:pmid	15019263
has license	no-cc
sha1sum (hex)	0c91f2c73d89deadccd0db5359eb1c95242ac027
schema:url	https://doi.org/10.1016/j.jviromet.2004.01.001
resource representing a document's title	Amplification refractory mutation system PCR assays for the detection of variola and Orthopoxvirus
has PubMed Central identifier	PMC7119807
has PubMed identifier	15019263
schema:publication	J Virol Methods
resource representing a document's body	covid:0c91f2c73d89deadccd0db5359eb1c95242ac027#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/0c91f2c73d89deadccd0db5359eb1c95242ac027
is schema:about of	named entity 'single nucleotide polymorphisms' named entity 'DNA' named entity 'variola virus' named entity 'mutation' named entity 'ORTHOPOXVIRUS' named entity 'DETECTION' named entity 'ASSAY' named entity 'EITHER' named entity 'DNA' named entity 'PANEL' named entity 'VARIOLA VIRUS DNA' named entity 'TESTED' named entity 'PRODUCTS' named entity 'ORTHOPOXVIRUS DNA' named entity 'CONSERVED' named entity 'VACCINIA VIRUS COPENHAGEN' named entity 'PROVIDED' named entity 'PROVIDE' named entity 'WORLD' named entity 'GENERIC' named entity 'SIGNATURE' named entity 'MULTIPLEX PCR' named entity 'SEQUENCES' named entity 'A36R' named entity 'USED' named entity 'THESE' named entity 'SAMPLES' named entity 'PCR' named entity 'PRESENCE OF' named entity 'GENES' named entity 'STRAIN' named entity 'COUNTRIES' named entity 'ARMS' named entity 'IDENTIFIED' named entity 'SPECIES IDENTIFICATION' named entity 'PRIMER' named entity 'RELIABLE' named entity 'ASSAY FORMAT' named entity 'ANALYSIS' named entity 'SPECIFIC' named entity 'ORTHOPOXVIRUS' named entity 'AMPLIFICATION REFRACTORY MUTATION SYSTEM ' named entity 'YEARS' named entity 'SECOND' named entity 'OUR' named entity 'VARIOLA VIRUS' named entity 'SPECIFICITY' named entity 'UNKNOWN' named entity 'UNIQUE' named entity 'IDENTIFY' named entity 'DID' named entity 'VARIOLA' named entity 'PCR' named entity '27S' named entity 'PRIMERS' named entity 'AMPLIFIED' named entity 'DIFFERENT' named entity 'POLYMORPHISMS' named entity 'MARKERS' named entity 'SINGLE NUCLEOTIDE POLYMORPHISMS' named entity 'COLLECTED' named entity 'HAVE' named entity 'STRAINS' named entity 'PCR PRODUCT' named entity 'PRODUCE' named entity 'USING' named entity 'GENOMES' named entity 'ESTABLISHED' named entity 'VIRUS IDENTIFICATION' named entity 'AMPLIFICATION REFRACTORY MUTATION SYSTEM ' named entity 'SAMPLE' named entity 'VARIOLA' named entity 'ACROSS' named entity 'CONSENSUS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software