About: Development and implementation of influenza a virus subtyping and detection of genotypic resistance to neuraminidase inhibitors

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About: Development and implementation of influenza a virus subtyping and detection of genotypic resistance to neuraminidase inhibitors Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and implementation of influenza a virus subtyping and detection of genotypic resistance to neuraminidase inhibitors
Creator	Casas, Inmaculada Reina, Jordi Pozo, Francisco Ruiz-Carrascoso, Guillermo Pé Rez-Breñ, Pilar Pé Rez-Gonzá, Carmen
Source	Medline; PMC
abstract	Influenza virus hemagglutinin and neuraminidase, surface glycoproteins with an essential role in viral pathogenesis, are important antigen determinants and essential markers for epidemiological surveillance. Neuraminidase is also a suitable target for designing antiviral drugs. The introduction into clinical practice of neuraminidase inhibitors and the development of random point mutations have increased the emergence of drug‐resistant viruses. A universal RT nested PCR‐based system has been developed for subtyping H1, H3, N1 and N2, in influenza A viruses of human or animal origin. The subsequent sequencing and analysis of the hemagglutinin and neuraminidase templates reveal antigenic and receptor binding changes in the HA1 subunit and mutations of clinical relevance concerning resistance to neuraminidase inhibitors. The specificity and sensitivity of the method were evaluated using 113 influenza A isolates, 105 influenza A positive respiratory samples obtained from patients and 29 prototype strains of both human and animal origin. The resulting analytical sensitivity of the subtyping techniques is one to at least 100 molecules of cloned DNA product in a final reaction volume of 50 µl. In the course of implementing the method, two H1N1 isolates with the H274Y mutation in the neuraminidase segment have been detected and their molecular features analyzed. The emergence of influenza virus resistance makes the neuraminidase genetic characterization and surveillance activities to detect antiviral resistance necessary. J. Med. Virol. 82: 843–853, 2010. © 2010 Wiley‐Liss, Inc.
has issue date	2010-03-24(xsd:dateTime)
bibo:doi	10.1002/jmv.21692
bibo:pmid	20336728
has license	no-cc
sha1sum (hex)	0d51b5d1fe18da0da930e3d1971b81d3cbe96982
schema:url	https://doi.org/10.1002/jmv.21692
resource representing a document's title	Development and implementation of influenza a virus subtyping and detection of genotypic resistance to neuraminidase inhibitors
has PubMed Central identifier	PMC7166748
has PubMed identifier	20336728
schema:publication	J Med Virol
resource representing a document's body	covid:0d51b5d1fe18da0da930e3d1971b81d3cbe96982#body_text
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