About: A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant
Creator	Zambon, Maria Baric, Ralph Gralinski, Lisa Agnihothram, Sudhakar Whitmore, Alan Yount, Boyd Donaldson, Eric Huynh, Jeremy Scobey, Trevor Heise, Mark Mesecar, Andrew Tomar, Sakshi Gopal, Robin Menachery, Vineet Denison, Mark Becker, Michelle Bl, Yount Ghosh, Arun Graham, Rachel Lipkin, Editor Osswald, Heather Vd, Menachery Agnihothram, Citation Ak, Ghosh Ef, Donaldson Hl, Osswald Le, Gralinski Mr, Denison Rl, Graham Td, Scobey
source	Medline; PMC
abstract	Cross-species transmission of zoonotic coronaviruses (CoVs) can result in pandemic disease outbreaks. Middle East respiratory syndrome CoV (MERS-CoV), identified in 2012, has caused 182 cases to date, with ~43% mortality, and no small animal model has been reported. MERS-CoV and Pipistrellus bat coronavirus (BtCoV) strain HKU5 of Betacoronavirus (β-CoV) subgroup 2c share >65% identity at the amino acid level in several regions, including nonstructural protein 5 (nsp5) and the nucleocapsid (N) protein, which are significant drug and vaccine targets. BtCoV HKU5 has been described in silico but has not been shown to replicate in culture, thus hampering drug and vaccine studies against subgroup 2c β-CoVs. We report the synthetic reconstruction and testing of BtCoV HKU5 containing the severe acute respiratory syndrome (SARS)-CoV spike (S) glycoprotein ectodomain (BtCoV HKU5-SE). This virus replicates efficiently in cell culture and in young and aged mice, where the virus targets airway and alveolar epithelial cells. Unlike some subgroup 2b SARS-CoV vaccines that elicit a strong eosinophilia following challenge, we demonstrate that BtCoV HKU5 and MERS-CoV N-expressing Venezuelan equine encephalitis virus replicon particle (VRP) vaccines do not cause extensive eosinophilia following BtCoV HKU5-SE challenge. Passage of BtCoV HKU5-SE in young mice resulted in enhanced virulence, causing 20% weight loss, diffuse alveolar damage, and hyaline membrane formation in aged mice. Passaged virus was characterized by mutations in the nsp13, nsp14, open reading frame 5 (ORF5) and M genes. Finally, we identified an inhibitor active against the nsp5 proteases of subgroup 2c β-CoVs. Synthetic-genome platforms capable of reconstituting emerging zoonotic viral pathogens or their phylogenetic relatives provide new strategies for identifying broad-based therapeutics, evaluating vaccine outcomes, and studying viral pathogenesis.
has issue date	2014-03-25(xsd:dateTime)
bibo:doi	10.1128/mbio.00047-14
bibo:pmid	24667706
has license	cc-by-nc-sa
sha1sum (hex)	0e2c97d34c8c6042f26cc11e83e1a2fbac521203
schema:url	https://doi.org/10.1128/mbio.00047-14
resource representing a document's title	A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant
has PubMed Central identifier	PMC3977350
has PubMed identifier	24667706
schema:publication	mBio
resource representing a document's body	covid:0e2c97d34c8c6042f26cc11e83e1a2fbac521203#body_text
is schema:about of	named entity 'MOUSE MODEL' named entity 'mortality' named entity 'SARS-CoV' named entity 'ectodomain' named entity 'aged' named entity 'outbreaks' named entity 'targets' named entity 'platforms' named entity 'weight loss' named entity 'particle' named entity 'demonstrate' named entity 'Subgroup' named entity 'NONSTRUCTURAL PROTEIN' named entity 'STRAIN' named entity 'BAT CORONAVIRUS' named entity 'DO NOT' named entity 'AIRWAY' named entity 'REPORTED' named entity 'VIRUS' named entity 'CONTAINING' named entity '90%' named entity 'CAUSING' named entity 'PROVIDE' named entity 'CELL CULTURE' named entity 'RESULT' named entity 'VENEZUELAN EQUINE ENCEPHALITIS VIRUS' named entity 'AGED' named entity 'EXTENSIVE' named entity 'REPLICON' named entity 'THERAPEUTICS' named entity 'GENOME' named entity 'VACCINE' named entity 'ANIMAL MODEL' named entity 'CHALLENGE' named entity 'REPLICATE' named entity 'IN SILICO' named entity 'FORMATION' named entity '28S' named entity 'SPECIES' named entity 'RELATIVES' named entity 'VACCINES' named entity 'PIPISTRELLUS' named entity 'ELICIT' named entity 'EVALUATING' named entity 'VIRAL PATHOGENESIS' named entity 'EOSINOPHILIA' named entity 'INCLUDING' named entity 'YOUNG' named entity 'VIRULENCE' named entity 'STRATEGIES' named entity 'BASED' named entity 'INHIBITOR'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software