About: Expression and membrane integration of SARS-CoV M protein

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About: Expression and membrane integration of SARS-CoV M protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression and membrane integration of SARS-CoV M protein
Creator	Li, Hui-Chun Ma, Hsin-Chieh Ae, Chen Hsieh, Yi-Ching Lo, Ae Shih-Yen Shih-Chi, A Ae, Fang Chiu-Ping, A
Source	Medline; PMC
abstract	SARS-CoV M gene fragment was cloned and expressed as a recombinant protein fused with a V5 tag at the C-terminus in Vero E6 cells. In addition to un-glycosylated and glycosylated proteins, one product with smaller size initiated in-frame from the third Met residues probably through ribosomal re-initiation was also detected. Translation initiated in-frame from the third Met is unusual since the sequence around the first Met of SARS-CoV M protein contains the optimal consensus Kozak sequence. The function of this smaller translated product awaits further investigation. Similar to other N-glycosylated proteins, glycosylation of SARS-CoV M protein was occurred co-translationally in the presence of microsomes. The SARS-CoV M protein is predicted as a triple-spanning membrane protein lack of a conventional signal peptide. The second and third trans-membrane regions (a.a. 46–68 and 78–100) are predicted to be the primary type helices, which will be able to penetrate into membrane by themselves, while the first trans-membrane region (a.a. 14–36) is predicted to be the secondary type helix, which is considered to be stabilized by the interaction with other trans-membrane segments. As expected, the second and third trans-membrane regions were able to insert a cytoplasmic protein into the endoplasmic reticulum membrane more efficiently than the first one. These results should be important for the study of SARS-CoV morphogenesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11373-008-9235-1) contains supplementary material, which is available to authorized users.
has issue date	2008-04-09(xsd:dateTime)
bibo:doi	10.1007/s11373-008-9235-1
bibo:pmid	18398701
has license	no-cc
sha1sum (hex)	0e4d1c0fdd86f53350ee549062cee1f6a7ac9fd1
schema:url	https://doi.org/10.1007/s11373-008-9235-1
resource representing a document's title	Expression and membrane integration of SARS-CoV M protein
has PubMed Central identifier	PMC7088832
has PubMed identifier	18398701
schema:publication	J Biomed Sci
resource representing a document's body	covid:0e4d1c0fdd86f53350ee549062cee1f6a7ac9fd1#body_text
is schema:about of	named entity 'proteins' named entity 'cloned' named entity 'primary' named entity 'Met' named entity 'microsomes' named entity 'Expression' named entity 'SEQUENCE' named entity 'FRAME' named entity 'FUNCTION' named entity 'PROTEIN FUSED' named entity 'SARS-COV' named entity 'PRIMARY' named entity 'INTERACTION' named entity 'OPTIMAL' named entity 'SIMILAR' named entity 'MICROSOMES' named entity 'UNUSUAL' named entity 'M GENE' named entity 'PRESENCE OF' named entity 'SECONDARY' named entity 'STUDY' named entity 'MEMBRANE PROTEIN' named entity 'SMALLER' named entity 'EXPRESSED' named entity 'INSERT' named entity 'MEMBRANE REGION' named entity 'GLYCOSYLATED' covid:arg/0e4d1c0fdd86f53350ee549062cee1f6a7ac9fd1 named entity 'CLONED' named entity 'M PROTEIN' named entity 'EXPRESSION' named entity 'MEMBRANE' named entity 'SARS-COV' named entity 'INTEGRATION' named entity 'SECOND' named entity 'SIZE' named entity 'HELIX' named entity 'M PROTEIN' named entity 'PRODUCT' named entity 'PROBABLY' named entity 'AS EXPECTED' named entity 'ADDITION' named entity 'HELICES' named entity 'ENDOPLASMIC RETICULUM MEMBRANE' named entity 'PENETRATE' named entity 'PROTEIN ' named entity 'REGIONS' named entity 'INITIATION' named entity '28A' named entity 'RIBOSOMAL' named entity 'MORPHOGENESIS' named entity 'VERO' named entity 'SEGMENTS' named entity 'C-TERMINUS' named entity 'TYPE' named entity 'MEMBRANE' named entity 'RECOMBINANT PROTEIN' named entity 'IMPORTANT' named entity '100%' named entity 'SIGNAL PEPTIDE' named entity 'CELLS' named entity 'MET' named entity 'TRANS' named entity 'GLYCOSYLATION' named entity 'TRANSLATED' named entity 'LACK' named entity 'TRIPLE' named entity 'FUSED WITH' named entity 'DETECTED' named entity 'V5 TAG' named entity 'CYTOPLASMIC PROTEIN' named entity 'GENE FRAGMENT' named entity 'KOZAK SEQUENCE' named entity 'TRANSLATION'

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