About: Transmission of Mouse Minute Virus (MMV) but Not Mouse Hepatitis Virus (MHV) Following Embryo Transfer with Experimentally Exposed In Vivo-Derived Embryos(1)

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About: Transmission of Mouse Minute Virus (MMV) but Not Mouse Hepatitis Virus (MHV) Following Embryo Transfer with Experimentally Exposed In Vivo-Derived Embryos(1) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Transmission of Mouse Minute Virus (MMV) but Not Mouse Hepatitis Virus (MHV) Following Embryo Transfer with Experimentally Exposed In Vivo-Derived Embryos(1)
Creator	Nauwynck, Hans Soom, Ann Bulian, Diana Mahabir, Esther Mateusen, Bart Mayer, Anna Needham, Jeffrey Rg Schmidt, Jö
topic	covid:0ed1304d3b9475096ecb0d57411f96892b8d2a5e#this
Source	Medline; PMC
abstract	The present study investigated the presence and location of fluorescent microspheres having the size of mouse hepatitis virus (MHV) and of mouse minute virus (MMV) in the zona pellucida (ZP) of in vivo-produced murine embryos, the transmission of these viruses by embryos during embryo transfer, and the time of seroconversion of recipients and pups. To this end, fertilized oocytes and morulae were exposed to different concentrations of MMVp for 16 h, while 2-cell embryos and blastocysts were coincubated for 1 h. In addition, morulae were exposed to MHV-A59 for 16 h. One group of embryos was washed, and the remaining embryos remained unwashed before embryo transfer. Serological analyses were performed by means of ELISA to detect antibodies to MHV or MMV in recipients and in progeny on Days 14, 21, 28, 42, and 63 and on Days 42, 63, 84, 112, 133, and 154, respectively, after embryo transfer. Coincubation with a minimum of 10(5)/ml of fluorescent microspheres showed that particles with a diameter of 20 nm but not 100 nm crossed the ZP of murine blastocysts. Washing generally led to a 10-fold to 100-fold reduction of MMVp. Washed MMV-exposed but not MHV-exposed embryos led to the production of antibodies independent of embryonic stage and time of virus exposure. Recipients receiving embryos exposed to a minimum of 10(7) mean tissue culture infective dose (TCID(50))/ml of MHV-A59 and 10(2) TCID(50)/ml of MMVp seroconverted by Day 42 after embryo transfer. The results indicate that MMV but not MHV can be transmitted to recipients even after washing embryos 10 times before embryo transfer.
has issue date	2007-02-01(xsd:dateTime)
bibo:doi	10.1095/biolreprod.106.056135
bibo:pmid	17021342
has license	no-cc
sha1sum (hex)	0ed1304d3b9475096ecb0d57411f96892b8d2a5e
schema:url	https://doi.org/10.1095/biolreprod.106.056135
resource representing a document's title	Transmission of Mouse Minute Virus (MMV) but Not Mouse Hepatitis Virus (MHV) Following Embryo Transfer with Experimentally Exposed In Vivo-Derived Embryos(1)
has PubMed Central identifier	PMC7109837
has PubMed identifier	17021342
schema:publication	Biol Reprod
resource representing a document's body	covid:0ed1304d3b9475096ecb0d57411f96892b8d2a5e#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/0ed1304d3b9475096ecb0d57411f96892b8d2a5e
is schema:about of	named entity 'virus' named entity 'embryos' named entity 'embryo transfer' named entity 'fertilized' named entity 'embryo transfer' named entity 'blastocysts' named entity 'microspheres' named entity 'The' named entity 'independent' named entity 'PARTICLES' named entity 'INFECTIVE DOSE' named entity 'VIRUS' named entity 'A59' named entity 'PRODUCTION' named entity 'MEANS' named entity 'TRANSMITTED' named entity 'WASHED' named entity 'MOUSE MINUTE VIRUS' named entity 'FOLD' named entity 'IN VIVO' named entity 'TIMES' named entity 'BUT' named entity 'GROUP' named entity 'TCID' named entity 'MURINE' named entity 'MOUSE HEPATITIS VIRUS' named entity 'OOCYTES' named entity 'MMVP' named entity '100' named entity '154' named entity '112' named entity 'EMBRYOS' named entity 'ZONA PELLUCIDA' named entity 'CELL EMBRYOS' named entity 'GENERALLY' named entity 'WASHING' named entity 'CONCENTRATIONS' named entity 'REMAINING' named entity 'PUPS' named entity 'RESULTS' named entity 'RECEIVING' named entity 'EXPOSED TO' named entity 'LOCATION' named entity 'TRANSMISSION' named entity 'DAY' named entity 'TISSUE CULTURE' named entity 'DIFFERENT' named entity 'MMV' named entity 'MICROSPHERES' named entity 'DAYS' named entity 'LED' named entity 'CROSSED' named entity 'ADDITION' named entity 'TIME' named entity 'THESE' named entity 'PRESENCE' named entity 'STUDY' named entity 'EMBRYONIC STAGE' named entity 'MINIMUM' named entity 'SEROLOGICAL' named entity 'FLUORESCENT' named entity 'EXPOSED' named entity '133' named entity '2-CELL' named entity 'END' named entity 'PERFORMED BY' named entity 'ELISA' named entity 'FERTILIZED' named entity 'EXPOSURE' named entity 'REDUCTION' named entity 'MEAN' named entity 'PROGENY'

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