About: The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice (Glycyrrhiza glabra)

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About: The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice (Glycyrrhiza glabra) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice (Glycyrrhiza glabra)
Creator	Mehrabi, Rahim Nasrollahi, Vida Nazeri, Sonbol Piri, Khosro Mirzaie-Asl, Asghar
Source	Elsevier; Medline; PMC
abstract	Abstract Glycyrrhiza glabra is an important medicinal plant throughout the world. Glycyrrhizin is a triterpenoid that is among the most important secondary metabolites produced by liquorice. Drought stress is proposed to enhance the levels of secondary metabolites. In this study, the effect of drought stress on the expression of important genes involved in the glycyrrhizin biosynthetic pathway was examined. Drought stress at the seedling stage was applied to 8-day-old plants using polyethylene glycol. Subsequently, the samples were collected 0, 4, 8 or 24h post-treatment. At the adult plant stage, 10-month-old plants were subjected to drought stress by discontinuing irrigation. Subsequently, samples were collected at 2, 16 and 28days after drought imposition (S2d, S16d and S28d, respectively). We performed semi-quantitative RT-PCR assays to evaluate the gene expression levels of sequalene synthase (SQS), β-amyrin synthase (bAS), lupeol synthase (LUS) and cycloartenol synthase (CAS) during stress. Finally, the glycyrrhizin content of stolons was determined via HPLC. The results revealed that due to osmotic stress, the gene expression levels of SQS and bAS were increased, whereas those of CAS were relatively unchanged at the seedling stage. At the adult plant stage, the expression levels of SQS and bAS were increased under drought stress conditions, whereas the gene expression level of CAS remained relatively constant. The glycyrrhizin content in stolons was increased only under severe drought stress conditions (S28d). Our results indicate that application of controlled drought stress up-regulates the expression of key genes involved in the biosynthesis of triterpenoid saponins and directly enhances the production of secondary metabolites, including glycyrrhizin, in liquorice plants.
has issue date	2014-07-31(xsd:dateTime)
bibo:doi	10.1016/j.phytochem.2014.03.004
bibo:pmid	24768283
has license	els-covid
sha1sum (hex)	1008493bc8f2024ea3dbc238e0fb3be0f6ef79c5
schema:url	https://doi.org/10.1016/j.phytochem.2014.03.004
resource representing a document's title	The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice (Glycyrrhiza glabra)
has PubMed Central identifier	PMC7111703
has PubMed identifier	24768283
schema:publication	Phytochemistry
resource representing a document's body	covid:1008493bc8f2024ea3dbc238e0fb3be0f6ef79c5#body_text
is schema:about of	named entity 'polyethylene glycol' named entity 'gene' named entity 'revealed' named entity 'involved' named entity 'plants' named entity 'plants' named entity 'key' named entity 'genes' named entity 'liquorice' named entity 'TRITERPENOID SAPONINS' named entity 'LIQUORICE' named entity 'BIOSYNTHETIC' named entity 'IMPORTANT' named entity 'IS A' named entity 'POST-TREATMENT' named entity 'INCLUDING' named entity 'DETERMINED' named entity 'IMPOSITION' named entity 'PROPOSED' named entity 'CONTROLLED' named entity 'EXAMINED' named entity '28S' named entity 'PLANTS' named entity 'OUR' named entity 'TRITERPENOID SAPONINS' named entity 'OLD' named entity 'BAS' named entity 'CYCLOARTENOL SYNTHASE' named entity 'SAMPLES' named entity 'OSMOTIC STRESS' named entity 'MONTH' named entity 'CONTENT' named entity 'BIOSYNTHESIS' named entity 'DROUGHT' named entity 'DURING STRESS' named entity 'APPLICATION' named entity 'USING' named entity 'PERFORMED' named entity 'HPLC' named entity 'DROUGHT STRESS' named entity 'GLYCYRRHIZIN' named entity 'CAS' named entity 'QUANTITATIVE RT-PCR' named entity 'ADULT' named entity 'EXPRESSION' named entity 'SEEDLING' named entity 'GENE EXPRESSION' named entity 'DAYS' named entity 'SEMI-QUANTITATIVE' named entity 'SYNTHASE' named entity 'TRITERPENOID' named entity 'COLLECTED' named entity 'MEDICINAL PLANT' named entity 'EVALUATE' named entity 'INCREASED' named entity 'DAY-OLD' named entity 'PLANT STAGE' named entity 'IRRIGATION' named entity 'EFFECT' named entity 'GENES' named entity 'CONDITIONS' named entity 'INVOLVED' named entity 'INVOLVED' named entity 'EXPRESSION' named entity 'BIOSYNTHESIS' named entity 'KEY' named entity 'STAGE' named entity 'SECONDARY METABOLITES' named entity 'UP-REGULATES' named entity 'POLYETHYLENE GLYCOL' named entity 'STUDY' named entity 'PRODUCTION' named entity 'EFFECT' named entity 'PATHWAY' named entity 'GLYCYRRHIZA GLABRA' named entity 'LUPEOL SYNTHASE' named entity 'LEVELS'

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