About: In situ hybridization technique for the detection of swine enteric and respiratory coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in formalin-fixed paraffin-embedded tissues

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: In situ hybridization technique for the detection of swine enteric and respiratory coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in formalin-fixed paraffin-embedded tissues Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In situ hybridization technique for the detection of swine enteric and respiratory coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in formalin-fixed paraffin-embedded tissues
Creator	Sirinarumitr, Theerapol Halbur, Patrick Kluge, John Paulb, Prem
Source	Elsevier; Medline; PMC
abstract	Abstract The in situ hybridization (ISH) technique was developed to detect the swine coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in cell culture and tissue sections from TGEV-or PRCV-infected pigs. The 35S-labeled RNA probes were generated from two plasmids pPSP.FP1 and pPSP.FP2 containing part of the S gene of TGEV. The procedure was first standardized in cell cultures. The radiolabeled pPSP.FP2 probe detected both TGEV and PRCV in virus-inoculated cell cultures, whereas pPSP.FP1 probe detected TGEV but not PRCV. The probe was then used to detect TGEV or PRCV in tissues of pigs experimentally infected with TGEV or PRCV or naturally infected with TGEV. Again, the probes detected TGEV in intestines of experimentally and naturally infected pigs and PRCV in the lungs of experimentally infected pigs. TGEV RNA was detected mainly within the enterocytes at the tips of villi and, less often, within some crypt epithelial cells. PRCV was shown to replicate mainly in the bronchiolar epithelial cells and in lesser amount in type II pneumocytes, type I pneumocytes, alveolar macrophages and bronchial epithelial cells, respectively. ISH has potential applications as a diagnostic test for the detection and differentiation of TGEV and PRCV in tissues and in studies to gain a better understanding of the mechanism of pathogenesis of enteric and respiratory coronavirus infections.
has issue date	1996-02-29(xsd:dateTime)
bibo:doi	10.1016/0166-0934(95)01901-4
bibo:pmid	8882645
has license	els-covid
sha1sum (hex)	10d26e0d6a3d50bd821ebc7c1588cb48d88431ee
schema:url	https://doi.org/10.1016/0166-0934%2895%2901901-4
resource representing a document's title	In situ hybridization technique for the detection of swine enteric and respiratory coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in formalin-fixed paraffin-embedded tissues
has PubMed Central identifier	PMC7119765
has PubMed identifier	8882645
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:10d26e0d6a3d50bd821ebc7c1588cb48d88431ee#body_text
is schema:about of	named entity 'RESPIRATORY' named entity 'TISSUES' named entity 'ISH' named entity 'naturally' named entity 'pathogenesis' named entity 'alveolar' named entity 'bronchial epithelial cells' named entity 'transmissible' named entity 'PORCINE RESPIRATORY CORONAVIRUS' named entity 'TISSUES' named entity 'PART OF' named entity 'TIPS' named entity 'DIFFERENTIATION' named entity 'RESPIRATORY' named entity 'POTENTIAL' named entity 'CORONAVIRUS INFECTIONS' named entity '35S' named entity 'INTESTINES' named entity 'ENTEROCYTES' named entity 'CORONAVIRUSES' named entity 'GENE' named entity 'BRONCHIOLAR' named entity 'BETTER' named entity 'RNA PROBES' named entity 'SWINE' named entity 'VIRUS' named entity 'DIAGNOSTIC TEST' named entity 'PLASMIDS' named entity 'EPITHELIAL CELLS' named entity 'REPLICATE' named entity 'PORCINE RESPIRATORY CORONAVIRUS' named entity 'DETECTED' named entity 'INFECTED' named entity 'IN SITU HYBRIDIZATION' named entity 'TISSUE' named entity 'DETECTION' named entity 'TYPE I' named entity 'PIGS' named entity 'CELL CULTURE' named entity 'BRONCHIAL' named entity 'GENERATED' named entity 'DETECTION' named entity 'LABELED' named entity 'THE LUNGS' named entity 'CELL CULTURES' named entity 'BUT' named entity 'PROCEDURE' named entity 'CRYPT' covid:arg/10d26e0d6a3d50bd821ebc7c1588cb48d88431ee named entity 'CORONAVIRUSES' named entity 'PROBES' named entity 'APPLICATIONS' named entity 'TYPE II' named entity 'PNEUMOCYTES' named entity 'ENTERIC' named entity 'SWINE' named entity 'FORMALIN-FIXED PARAFFIN-EMBEDDED' named entity 'LESSER' named entity 'ISH' named entity 'LESS OFTEN' named entity 'CONTAINING' named entity 'RADIOLABELED' named entity 'PROBE' named entity 'TECHNIQUE' named entity 'MECHANISM OF PATHOGENESIS' named entity 'UNDERSTANDING' named entity 'STUDIES' named entity 'TO DETECT' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'RNA' named entity 'GAIN' named entity 'USED' named entity 'ENTERIC' named entity 'HYBRIDIZATION TECHNIQUE' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'IN SITU HYBRIDIZATION' named entity 'ALVEOLAR MACROPHAGES' named entity 'replicate'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software