About: Application of quantitative PCR for the detection of microorganisms in water

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Application of quantitative PCR for the detection of microorganisms in water Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Application of quantitative PCR for the detection of microorganisms in water
Creator	Botes, Marelize Cloete, Thomas De Kwaadsteniet, Michéle
Source	Medline; PMC
abstract	The occurrence of microorganisms in water due to contamination is a health risk and control thereof is a necessity. Conventional detection methods may be misleading and do not provide rapid results allowing for immediate action. The quantitative polymerase chain reaction (qPCR) method has proven to be an effective tool to detect and quantify microorganisms in water within a few hours. Quantitative PCR assays have recently been developed for the detection of specific adeno- and polyomaviruses, bacteria and protozoa in different water sources. The technique is highly sensitive and able to detect low numbers of microorganisms. Quantitative PCR can be applied for microbial source tracking in water sources, to determine the efficiency of water and wastewater treatment plants and act as a tool for risk assessment. Different qPCR assays exist depending on whether an internal control is used or whether measurements are taken at the end of the PCR reaction (end-point qPCR) or in the exponential phase (real-time qPCR). Fluorescent probes are used in the PCR reaction to hybridise within the target sequence to generate a signal and, together with specialised systems, quantify the amount of PCR product. Quantitative reverse transcription polymerase chain reaction (q-RT-PCR) is a more sensitive technique that detects low copy number RNA and can be applied to detect, e.g. enteric viruses and viable microorganisms in water, and measure specific gene expression. There is, however, a need to standardise qPCR protocols if this technique is to be used as an analytical diagnostic tool for routine monitoring. This review focuses on the application of qPCR in the detection of microorganisms in water.
has issue date	2012-09-25(xsd:dateTime)
bibo:doi	10.1007/s00216-012-6399-3
bibo:pmid	23001336
has license	no-cc
sha1sum (hex)	10ee080308e7ee4e7e6223c75f4666b096e469e5
schema:url	https://doi.org/10.1007/s00216-012-6399-3
resource representing a document's title	Application of quantitative PCR for the detection of microorganisms in water
has PubMed Central identifier	PMC7079929
has PubMed identifier	23001336
schema:publication	Anal Bioanal Chem
resource representing a document's body	covid:10ee080308e7ee4e7e6223c75f4666b096e469e5#body_text
is schema:about of	named entity 'The' named entity 'water' named entity 'quantify' named entity 'microorganisms' named entity 'SOURCES' named entity 'BACTERIA ' named entity 'APPLIED' named entity 'detection' named entity 'microbial' named entity 'effective' named entity 'phase' named entity 'generate' named entity 'water' named entity 'microorganisms' named entity 'Quantitative PCR' named entity 'rapid' named entity 'detect' named entity 'contamination' named entity 'expression' named entity 'exponential' named entity 'numbers' named entity 'Quantitative' named entity 'detects' named entity 'measure' named entity 'detection' named entity 'RNA' named entity 'microorganisms' named entity 'enteric viruses' named entity 'risk assessment' named entity 'PCR reaction' named entity 'microorganisms' named entity 'reverse transcription polymerase chain reaction' named entity 'bacteria' named entity 'exponential phase' named entity 'diagnostic tool' named entity 'Quantitative PCR' named entity 'qPCR' named entity 'RT-PCR' named entity 'DNA sequence' named entity 'water safety plan' named entity 'damaged cells' named entity 'water samples' named entity 'Cysts' named entity '100 %' named entity '5′ to 3′' named entity 'Gel electrophoresis' named entity 'qPCR' named entity 'faecal contamination' named entity 'wastewater' named entity 'messenger RNA' named entity 'enteric viruses' named entity 'microalgal' named entity 'qPCR' named entity 'diarrhoea' named entity 'covalently linked' named entity 'disease outbreaks' named entity 'surface water' named entity 'blue circle' named entity 'microbial cells' named entity 'qPCR' named entity 'Clostridium' named entity 'primers' named entity 'biosolids' named entity 'nuclease' named entity 'wastewater' named entity 'RNA genomes' named entity 'nucleic acid sequence' named entity 'serotype' named entity 'supply of water' named entity 'qPCR' named entity 'fluorescence' named entity 'waterborne pathogens' named entity 'inoculated' named entity 'real-time PCR' named entity 'Cholera' named entity 'PCR' named entity 'Viruses' named entity 'spp' named entity 'safe drinking water'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software