About: Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR

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About: Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR
Creator	Chiou, Ming-Tang Lin, Chao-Nan Hung, Li-Ning Lin, Wei-Hao Wang, Sheng-Yuan
Source	PMC
abstract	BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) is a RNA virus with high genetic variation. This virus causes significant economic losses in most pig-producing countries. The clinical presentation of PRRSV ranges from asymptomatic to devastating. In this study, we developed a sensitive and specific zip nucleic acid probe-based real-time PCR assay to evaluate the viremia of natural PRRSV-infected pigs in Taiwan. Serum samples were collected from 577 pigs aged 5–12 weeks. These include 444 clinically healthy pigs and 133 symptomatic pigs were confirmed to have porcine respiratory disease complex (PRDC). RESULTS: Viremia was quantified in 79 of the 444 (17.8%) clinically healthy pigs and in 112 of the 133 (84.2%) PRDC cases. Viremias were significantly more common in pigs with PRDC compared with the clinically healthy pigs (P <0.0001). These results suggest that a high viral load is a major feature of PRRSV-affected pigs. CONCLUSIONS: ZNA probe-based real-time PCR can be a useful tool to diagnose symptomatic and asymptomatic PRRSV-infected pigs. The presence of this marker in a sample of animals with high PRRSV loads (>10(4.2) PRRSV genomes/μl of serum) seems to indicate that it correlates with the presence of PRDC in pigs.
has issue date	2013-09-12(xsd:dateTime)
bibo:doi	10.1186/1746-6148-9-181
bibo:pmid	24028493
has license	cc-by
sha1sum (hex)	112c03868171e79e5f64b72e7c837fd4875d88f4
schema:url	https://doi.org/10.1186/1746-6148-9-181
resource representing a document's title	Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR
has PubMed Central identifier	PMC3847877
has PubMed identifier	24028493
schema:publication	BMC Vet Res
resource representing a document's body	covid:112c03868171e79e5f64b72e7c837fd4875d88f4#body_text
is schema:about of	named entity 'high' named entity 'pigs' named entity 'real-time PCR' named entity 'respiratory' named entity 'quantified' named entity 'viral load' named entity 'aged' named entity 'porcine' named entity 'respiratory' covid:arg/112c03868171e79e5f64b72e7c837fd4875d88f4 named entity 'weeks' named entity 'pigs' named entity 'RNA virus' named entity 'genomes' named entity 'study' named entity 'Taiwan' named entity 'serum' named entity 'PRRSV' named entity 'pig' named entity 'nucleic acid probe' named entity 'primer' named entity 'PRRSV' named entity 'porcine parvovirus' named entity 'immunohistochemistry' named entity 'Nsp2' named entity 'causative agent' named entity 'gene' named entity 'plasmid DNA' named entity 'plasmid DNA' named entity 'chi-square test' named entity 'asymptomatic' named entity 'infection' named entity 'PRRSV' named entity 'coefficient of variation' named entity 'PRRSV' named entity 'porcine circovirus' named entity 'PRRSV' named entity 'asymptomatic' named entity 'ORF' named entity 'asymptomatic' named entity 'gene' named entity 'virus isolation' named entity 'genotype' named entity 'real-time PCR' named entity 'PCR' named entity 'virus' named entity 'dyspnea' named entity 'PRRS' named entity 'PRRSV' named entity 'National Pingtung University' named entity '4.21' named entity 'nucleic acid' named entity 'porcine' named entity 'serum' named entity 'correlation' named entity 'standard curve' named entity 'RNA extraction' named entity 'oligonucleotides' named entity 'chi-square test' named entity '2.11' named entity 'PCR' named entity 'real-time PCR' named entity 'primer' named entity 'PRRSV' named entity 'PRDC' named entity 'reverse transcription' named entity 'perfect match' named entity 'serological' named entity 'PRDC' named entity 'serum' named entity 'genetic variation' named entity 'PRDC' named entity 'type 1' named entity 'Nidovirales' named entity 'serum samples' named entity 'real-time PCR' named entity 'PRRSV'

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