About: Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR

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About: Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR—A GA2LEN project Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR—A GA2LEN project
Creator	Molenkamp, Richard Kurowski, Marcin Papadopoulos, Nikolaos Kowalski, Marek Carlsen, Kai-Håkon Christodoulou, Ioannis De Beer, Lieke Hovland, Vegard Lødrup-Carlsen, Karin Olszewska-Ziąber, Agnieszka Spyridaki, Irini Van Drunen, Cornelis
Source	Elsevier; Medline; PMC
abstract	Abstract The aim of this study was to compare the efficacy and patient discomfort between four techniques for obtaining nasal secretions. Nasal secretions from 58 patients with symptoms of a common cold, from three clinical centers (Amsterdam, Lodz, Oslo), were obtained by four different methods: swab, aspirate, brush, and wash. In each patient all four sampling procedures were performed and patient discomfort was evaluated by a visual discomfort scale (scale 1–5) after each procedure. Single pathogen RT-PCRs for Rhinovirus (RV), Influenza virus and Adenovirus, and multiplex real-time PCR for RV, Enterovirus, Influenza virus, Adenovirus, Respiratory Syncytial Virus (RSV), Parainfluenza virus, Coronavirus, Metapneumovirus, Bocavirus and Parechovirus were performed in all samples. A specific viral cause of respiratory tract infection was determined in 48 patients (83%). In these, the detection rate for any virus was 88% (wash), 79% (aspirate), 77% (swab) and 74% (brush). The degree of discomfort reported was 2.54 for swabs, 2.63 for washes, 2.68 for aspirates and 3.61 for brushings. Nasal washes yielded the highest rate of viral detection without excessive patient discomfort. In contrast, nasal brushes produced the lowest detection rates and demonstrated the highest level of discomfort.
has issue date	2009-03-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.10.027
bibo:pmid	19041346
has license	els-covid
sha1sum (hex)	117e3db50cab4495375ac155a250283173f51757
schema:url	https://doi.org/10.1016/j.jviromet.2008.10.027
resource representing a document's title	Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR—A GA2LEN project
has PubMed Central identifier	PMC7112903
has PubMed identifier	19041346
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:117e3db50cab4495375ac155a250283173f51757#body_text
is schema:about of	named entity 'wash' named entity 'Oslo' named entity 'Single' named entity 'Amsterdam' named entity 'respiratory tract' named entity 'LEVEL' named entity 'TO COMPARE' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'MULTIPLEX REAL-TIME PCR' named entity 'COMMON COLD' named entity '2.68' named entity 'PROCEDURE' named entity 'SYMPTOMS' named entity 'SCALE' named entity 'OBTAINED BY' named entity 'REAL-TIME PCR ' named entity 'DEMONSTRATED' named entity 'DETECTION' named entity 'SAMPLING' named entity 'CAUSE' named entity 'RHINOVIRUS' named entity 'NASAL' named entity 'CLINICAL' named entity 'EVALUATED' named entity 'BRUSH' named entity 'VIRAL DETECTION' named entity 'DETECTION' named entity 'NASAL' named entity 'COMPARISON' named entity 'PROJECT' named entity 'LEN' named entity 'GA 2' named entity 'RT-PCR' named entity 'VIRAL PATHOGENS' named entity 'SAMPLING' named entity 'METHODS' named entity 'EFFICACY' named entity 'PROCEDURES' named entity 'DEGREE' named entity 'NASAL SECRETIONS' named entity 'PARECHOVIRUS' named entity 'DETERMINED' named entity 'HIGHEST' named entity 'VIRAL' named entity 'LODZ' named entity 'TECHNIQUES' named entity 'PERFORMED' named entity 'DISCOMFORT' named entity 'PARAINFLUENZA VIRUS' named entity 'ENTEROVIRUS' named entity 'REPORTED' named entity 'SINGLE' named entity 'CENTERS' named entity 'PATIENT' named entity 'CORONAVIRUS' named entity 'ADENOVIRUS' named entity 'EXCESSIVE' named entity 'SWABS' named entity 'THESE' named entity 'RESPIRATORY TRACT INFECTION' named entity 'A COMMON' named entity 'DIFFERENT' named entity 'METAPNEUMOVIRUS' named entity 'RATE' named entity 'BOCAVIRUS' named entity 'STUDY' named entity 'VIRUS' named entity 'ASPIRATE' named entity 'LOWEST' named entity 'INFLUENZA VIRUS'

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