About: Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice

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About: Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice
Creator	Huang, Xiaobo Ma, Xiaoping Wen, Xintian Wen, Yiping Wu, Rui Liu, Jiawen Yan, Qigui Zhang, Yudi Chen, • Li, Yaqing Qing, Ying Sanjie Cao, •
source	Medline; PMC
abstract	Attenuated Salmonella typhimurium (S. typhimurium) was selected as a transgenic vehicle for the development of live mucosal vaccines against transmissible gastroenteritis virus (TGEV) based on the M gene. An approximate 1.0 kb DNA fragment, encoding for glycoprotein M, was amplified by RT-PCR and cloned into eukaryotic expression vector pVAX1. The recombinant plasmid pVAX-M was transformed by electroporation into attenuated S. typhimurium SL7207, and the expression and translation of the pVAX-M delivered by recombinant S. typhimurium SL7207 (pVAX-M) was detected both in vitro and in vivo. BALB/c mice were inoculated orally with SL7207 (pVAX-M) at different dosages to evaluate safety of the vaccines. The bacterium was safe to mice at a dosage of 2 × 10(9) CFU, almost eliminated from the spleen and liver at week 4 post-immunization and eventually cleared at week 6. Mice immunized with 1 × 10(9) CFU of SL7207 (pVAX-M) elicited specific anti-TGEV local mucosal and humoral responses including levels of IgA, IgG, IL-4, and IFN-γ as measured by indirect ELISA assay. Moreover, the control groups (pVAX group, PBS group) maintained at a normal level during week 4–8 post-immunization. The results indicated that attenuated S. typhimurium could be used as a delivery vector for oral immunization of TGEV M gene vaccine.
has issue date	2016-02-02(xsd:dateTime)
bibo:doi	10.1007/s11262-016-1296-z
bibo:pmid	26837896
has license	no-cc
sha1sum (hex)	11b32fd959131a10c460119368638178a512b2da
schema:url	https://doi.org/10.1007/s11262-016-1296-z
resource representing a document's title	Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice
has PubMed Central identifier	PMC7088643
has PubMed identifier	26837896
schema:publication	Virus Genes
resource representing a document's body	covid:11b32fd959131a10c460119368638178a512b2da#body_text
is schema:about of	named entity 'measured' named entity 'IgA' named entity 'cloned' named entity 'mucosal' named entity 'SAFE' named entity 'PBS' named entity 'INCLUDING' named entity 'LIVE' named entity 'LOCAL' named entity 'BASED' named entity 'IN VITRO' named entity 'CLONED' named entity 'VACCINES' named entity 'ELIMINATED' named entity 'IMMUNIZED' named entity 'GROUP' named entity 'COULD BE' named entity 'AMPLIFIED' named entity 'BALB' named entity 'ORALLY' named entity 'GLYCOPROTEIN' named entity 'ELICITED' named entity 'LEVELS' named entity 'RECOMBINANT' named entity 'IN VIVO' named entity 'DOSAGES' named entity 'SPECIFIC' named entity 'EUKARYOTIC' named entity 'LEVEL' named entity 'TRANSFORMED' named entity 'ATTENUATED' named entity 'ELECTROPORATION' named entity 'DELIVERED' named entity 'MUCOSAL' named entity 'VEHICLE' named entity 'VACCINE' named entity 'MAINTAINED' named entity 'DNA FRAGMENT' named entity '28S' named entity 'IGG' named entity 'MICE' named entity 'DEVELOPMENT' named entity 'USED' named entity 'TRANSLATION' named entity 'BACTERIUM' named entity 'DELIVERY' named entity 'ALMOST' named entity 'ENCODING' named entity 'INDIRECT' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'EXPRESSION VECTOR' named entity 'MEASURED' named entity 'ELISA ASSAY' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'M GENE' named entity 'IMMUNOGENICITY' named entity 'DELIVERED' named entity 'IGA' named entity 'ORAL IMMUNIZATION' named entity 'EVALUATE' named entity 'M GENE' named entity 'LIVER' named entity 'DETECTED' named entity 'DIFFERENT' named entity 'POST' named entity 'SELECTED' named entity 'RESULTS' named entity 'RT-PCR' named entity 'IFN' named entity 'CONTROL GROUPS'

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