About: Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus

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About: Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus
Creator	Cong, Feng Huang, Ren Guo, Pengju Wu, Miaoli Zeng, Fanwen Zhu, Yujun Huang, Bihong Yuan, Wen Ma, Lei Xu, Fengjiao
Source	Medline; PMC
abstract	BACKGROUND: Murine norovirus (MNV) is recognized as the most prevalent viral pathogen in captive mouse colonies. The rapid detection assay for MNV would be a useful tool for monitoring and preventing MNV infection. A recombinase polymerase amplification (RPA) assay was established in this study to provide a solution for rapid and sensitive detection of MNV. RESULTS: The detection limit of the RT-RPA assay for the detection of MNV was 1 × 10(2) copies of RNA molecules per reaction. The assay was specific since there was no cross-reaction with other common murine viruses. In addition, the broad reactivity of the RT-RPA assay was validated using the synthesized template carrying seven point mutations among several MNV strains. The MNV RT-RPA assay could detect as few as 1 × 10(2) copies of the mutant per reaction, suggesting the assay could be broadly reactive against a large diversity of MNV strains. Forty eight clinical samples including 16 gastric tissue specimens, 16 cecal tissue specimens and 16 fecal specimens were tested for the validation of the new developed RT-RPA assay. The detection results of RT-RPA and RT-qPCR for clinical samples were very similar, except that a gastric tissue sample which was positive by RT-qPCR, with a RNA titer of 27 copies, was negative by RT-RPA. CONCLUSIONS: A broadly reactive RT-RPA assay was successfully established for MNV detection.
has issue date	2018-12-14(xsd:dateTime)
bibo:doi	10.1186/s12917-018-1736-1
bibo:pmid	30547776
has license	cc-by
sha1sum (hex)	11f3022ad1def6dfb1b29712112b96739ff3c498
schema:url	https://doi.org/10.1186/s12917-018-1736-1
resource representing a document's title	Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus
has PubMed Central identifier	PMC6295035
has PubMed identifier	30547776
schema:publication	BMC Vet Res
resource representing a document's body	covid:11f3022ad1def6dfb1b29712112b96739ff3c498#body_text
is schema:about of	named entity 'ASSAY' named entity 'tool' named entity 'assay' named entity 'REACTIVE' named entity 'ASSAY' named entity 'SOLUTION' named entity 'RAPID' named entity 'SENSITIVE' named entity 'BACKGROUND' named entity 'PREVENTING' named entity 'VIRAL PATHOGEN' named entity 'TOOL' named entity 'INFECTION' named entity 'TO PROVIDE' named entity 'MOUSE' named entity 'MURINE NOROVIRUS' named entity 'DETECTION' named entity 'DEVELOPMENT' named entity 'REVERSE TRANSCRIPTION' named entity 'MNV' named entity 'POLYMERASE' named entity 'USEFUL' covid:arg/11f3022ad1def6dfb1b29712112b96739ff3c498 named entity 'RAPID' named entity 'DETECTION' named entity 'STUDY' named entity 'AMPLIFICATION' named entity 'EVALUATION' named entity 'MURINE NOROVIRUS' named entity 'AMPLIFICATION' named entity 'PREVALENT' named entity 'ESTABLISHED' named entity 'MONITORING' named entity 'RECOMBINASE' named entity 'POLYMERASE' named entity 'COLONIES' named entity 'RECOMBINASE' named entity 'solution' named entity 'preventing' named entity 'sensitive' named entity 'RPA' named entity 'assay' named entity 'MNV' named entity 'pneumonia' named entity 'distilled water' named entity 'binding site' named entity 'RPA' named entity 'amplicon' named entity 'titer' named entity 'sodium sulfate' named entity 'real-time RT-PCR' named entity 'primer design' named entity 'RPA' named entity 'RNA' named entity 'primers' named entity 'major capsid protein' named entity 'ORF2' named entity 'RNA' named entity 'Promega' named entity 'PVM' named entity 'virus' named entity 'quality control' named entity 'regression analysis' named entity '12.2' named entity 'RT-PCR' named entity 'RPA' named entity 'polymerase' named entity 'RT-qPCR' named entity 'pathogenic bacteria' named entity 'RPA' named entity 'RNA molecules' named entity 'gene amplification'

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