About: Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice

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About: Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice
Creator	Deng, Yao Tan, Wenjie Huang, Baoying Ye, Fei Zhao, Li Niu, Junwei Shen, Liang Wang, Huijuan
Source	Elsevier; Medline; PMC
abstract	Abstract Human coronaviruses (HCoVs) are important pathogens that cause upper respiratory tract infections and have neuroinvasive abilities; however, little is known about the dynamic infection process of CoVs in vivo, and there are currently no specific antiviral drugs to prevent or treat HCoV infection. Here, we verified the replication ability and pathogenicity of a reporter HCoV-OC43 strain expressing Renilla luciferase (Rluc; rOC43-ns2DelRluc) in mice with different genetic backgrounds (C57BL/6 and BALB/c). Additionally, we monitored the spatial and temporal progression of HCoV-OC43 through the central nervous system (CNS) of live BALB/c mice after intranasal or intracerebral inoculation with rOC43-ns2DelRluc. We found that rOC43-ns2DelRluc was fatal to suckling mice after intranasal inoculation, and that viral titers and Rluc expression were detected in the brains and spinal cords of mice infected with rOC43-ns2DelRluc. Moreover, viral replication was initially observed in the brain by non-invasive bioluminescence imaging before the infection spread to the spinal cord of BALB/c mice, consistent with its tropism in the CNS. Furthermore, the Rluc readout correlated with the HCoV replication ability and protein expression, which allowed quantification of antiviral activity in live mice. Additionally, we validated that chloroquine strongly inhibited rOC43-ns2DelRluc replication in vivo. These results provide new insights into the temporal and spatial dissemination of HCoV-OC43 in the CNS, and our methods provide an extremely sensitive platform for evaluating the efficacy of antiviral therapies to treat neuroinvasive HCoVs in live mice.
has issue date	2020-01-31(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2019.104646
bibo:pmid	31705922
has license	els-covid
sha1sum (hex)	1291897d17fac6a0658ddca49931aca2b7d2eb65
schema:url	https://doi.org/10.1016/j.antiviral.2019.104646
resource representing a document's title	Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice
has PubMed Central identifier	PMC7114176
has PubMed identifier	31705922
schema:publication	Antiviral Research
resource representing a document's body	covid:1291897d17fac6a0658ddca49931aca2b7d2eb65#body_text
is schema:about of	named entity 'viral replication' named entity 'CNS' named entity 'verified' named entity 'extremely' named entity 'suckling' named entity 'chloroquine' named entity 'inoculation' named entity 'spinal' named entity 'live' named entity 'NON-INVASIVE' named entity 'OUR' named entity 'INTRACEREBRAL' named entity 'REPORTER' named entity 'REPLICATION' named entity 'INSIGHTS' named entity 'VERIFIED' named entity 'CHLOROQUINE' named entity 'PROVIDE' named entity 'INFECTION PROCESS' named entity 'DYNAMIC' named entity 'PROTEIN EXPRESSION' named entity 'ANTIVIRAL DRUGS' named entity 'THESE' named entity 'READOUT' named entity 'OBSERVED' named entity 'DIFFERENT' named entity 'RENILLA LUCIFERASE' named entity 'ANTIVIRAL ACTIVITY' named entity 'TREAT' named entity 'BALB' named entity 'PROGRESSION' named entity 'VIRAL REPLICATION' named entity 'TEMPORAL' named entity 'ABILITY' named entity 'INTRANASAL INOCULATION' named entity 'MICE' named entity 'DISSEMINATION' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'CORONAVIRUSES' named entity 'TO PREVENT' named entity 'HUMAN' named entity 'NEW' named entity 'HCOV-OC43' named entity 'LIVE' named entity 'BIOLUMINESCENCE IMAGING' named entity 'INFECTION' named entity 'THERAPY' named entity 'MICE' named entity 'METHODS' named entity 'PLATFORM' named entity 'LIVE' named entity 'CAUSE' named entity 'TROPISM' named entity 'KNOWN' named entity 'FOUND' named entity 'EXPRESSION' named entity 'COVS' named entity 'BRAINS' named entity 'DETECTED' named entity 'HCOV-OC43' named entity 'CORRELATED' named entity 'MONITORED' named entity 'IN VIVO' named entity 'HAVE' named entity 'INFECTED' named entity 'THE BRAIN' named entity 'HERE' named entity 'QUANTIFICATION' named entity 'ITS' named entity 'SPECIFIC' named entity 'EXTREMELY' named entity 'STRAIN' named entity 'EFFICACY' named entity 'FATAL'

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