About: An RNA Aptamer Provides a Novel Approach for the Induction of Apoptosis by Targeting the HPV16 E7 Oncoprotein

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About: An RNA Aptamer Provides a Novel Approach for the Induction of Apoptosis by Targeting the HPV16 E7 Oncoprotein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An RNA Aptamer Provides a Novel Approach for the Induction of Apoptosis by Targeting the HPV16 E7 Oncoprotein
Creator	Forrest, Sophie Nicol, Clare Belyaeva, Tamara Blair, G Bunka, David Cesur, Zlem Stonehouse, Nicola
Source	Medline; PMC
abstract	BACKGROUND: Human papillomavirus 16 (HPV16) is a high-risk DNA tumour virus, which is a major causative agent of cervical cancer. Cellular transformation is associated with deregulated expression of the E6 and E7 oncogenes. E7 has been shown to bind a number of cellular proteins, including the cell cycle control protein pRb. In this study, RNA aptamers (small, single-stranded oligonucleotides selected for high-affinity binding) to HPV16 E7 were employed as molecular tools to further investigate these protein-protein interactions. METHODOLOGY/PRINCIPAL FINDINGS: This study is focused on one aptamer (termed A2). Transfection of this molecule into HPV16-transformed cells resulted in inhibition of cell proliferation (shown using real-time cell electronic sensing and MTT assays) due to the induction of apoptosis (as demonstrated by Annexin V/propidium iodide staining). GST-pull down and bead binding assays were used to demonstrate that the binding of A2 required N-terminal residues of E7 known to be involved in interaction with the cell cycle control protein, pRb. Using a similar approach, A2 was shown to disrupt the interaction between E7 and pRb in vitro. Furthermore, transfection of HPV16-transformed cells with A2 appeared to result in the loss of E7 and rise in pRb levels, as observed by immunoblotting. CONCLUSIONS/SIGNIFICANCE: This paper includes the first characterisation of the effects of an E7 RNA aptamer in a cell line derived from a cervical carcinoma. Transfection of cells with A2 was correlated with the loss of E7 and the induction of apoptosis. Aptamers specific for a number of cellular and viral proteins have been documented previously; one aptamer (Macugen) is approved for clinical use and several others are in clinical trials. In addition to its role as a molecular tool, A2 could have further applications in the future.
has issue date	2013-05-30(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0064781
bibo:pmid	23738000
has license	cc-by
sha1sum (hex)	1297127819be12e7f5319edd0a9b35fd5cc566b7
schema:url	https://doi.org/10.1371/journal.pone.0064781
resource representing a document's title	An RNA Aptamer Provides a Novel Approach for the Induction of Apoptosis by Targeting the HPV16 E7 Oncoprotein
has PubMed Central identifier	PMC3667794
has PubMed identifier	23738000
schema:publication	PLoS One
resource representing a document's body	covid:1297127819be12e7f5319edd0a9b35fd5cc566b7#body_text
is schema:about of	named entity 'Cellular' named entity 'protein-protein interactions' named entity 'HPV16' named entity 'Targeting' named entity 'Apoptosis' covid:arg/1297127819be12e7f5319edd0a9b35fd5cc566b7 named entity 'oligonucleotides' named entity 'DNA' named entity 'oncogenes' named entity 'high-risk' named entity 'HPV16' named entity 'RNA' named entity 'Aptamer' named entity 'Apoptosis' named entity 'pRb' named entity 'aptamer' named entity 'HeLa' named entity 'pRb' named entity 'RTCA' named entity 'aptamer' named entity 'ubiquitinated' named entity 'E6 and E7' named entity 'FITC' named entity 'lowest energy' named entity 'cellular proliferation' named entity 'point mutations' named entity 'dose-dependent' named entity 'p-values' named entity 'transfected' named entity 'transfection' named entity 'isopropanol' named entity 'transfected' named entity 'co-immunoprecipitation' named entity 'epithelia' named entity 'Expression vectors' named entity 'pRb' named entity 'pRb' named entity 'reverse transcriptase' named entity 'transfection' named entity 'staurosporine' named entity 'HPV' named entity 'SiHa' named entity 'MTT assay' named entity 'cell lysate' named entity 'HeLa' named entity 'aptamer' named entity 'RNA aptamers' named entity 'Human papillomaviruses' named entity 'HeLa' named entity 'conjugated' named entity 'pRb' named entity 'oncoprotein' named entity 'SiHa' named entity 'transcription' named entity 'cell line' named entity 'mode of action' named entity 'apoptosis' named entity 'pRb' named entity 'apoptosis' named entity 'pRb' named entity 'aptamer' named entity 'pRb' named entity 'aptamers' named entity 'RNA' named entity 'Transfection' named entity 'SiHa' named entity 'Cellular transformation' named entity 'transfection' named entity 'glutathione' named entity 'staurosporine' named entity 'transfected' named entity 'cytotoxic compounds' named entity 'HeLa' named entity 'pRb' named entity 'pRb'

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