About: Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A

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About: Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A
Creator	Lin, Chun-Ming Wang, Qiuhong Hou, Yixuan Baric, Ralph Yount, Boyd Beall, Anne Saif, Linda Lipkin, Editor Beall, Citation Lin, Yount
Source	Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic alphacoronavirus. In the United States, highly virulent PEDV strains cause between 80 and 100% mortality in suckling piglets and are rapidly transmitted between animals and farms. To study the genetic factors that regulate pathogenesis and transmission, we developed a molecular clone of PEDV strain PC22A. The infectious-clone-derived PEDV (icPEDV) replicated as efficiently as the parental virus in cell culture and in pigs, resulting in lethal disease in vivo. Importantly, recombinant PEDV was rapidly transmitted to uninoculated pigs via indirect contact, demonstrating virulence and efficient transmission while replicating phenotypes seen in the wild-type virus. Using reverse genetics, we removed open reading frame 3 (ORF3) and replaced this region with a red fluorescent protein (RFP) gene to generate icPEDV-ΔORF3-RFP. icPEDV-ΔORF3-RFP replicated efficiently in vitro and in vivo, was efficiently transmitted among pigs, and produced lethal disease outcomes. However, the diarrheic scores in icPEDV-ΔORF3-RFP-infected pigs were lower than those in wild-type-virus- or icPEDV-infected pigs, and the virus formed smaller plaques than those of PC22A. Together, these data describe the development of a robust reverse-genetics platform for identifying genetic factors that regulate pathogenic outcomes and transmission efficiency in vivo, providing key infrastructural developments for developing and evaluating the efficacy of live attenuated vaccines and therapeutics in a clinical setting.
has issue date	2016-01-05(xsd:dateTime)
bibo:doi	10.1128/mbio.01451-15
bibo:pmid	26733065
has license	cc-by-nc-sa
sha1sum (hex)	131af5ab50551638e3ce9a8c615b9b4364f2f34e
schema:url	https://doi.org/10.1128/mbio.01451-15
resource representing a document's title	Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A
has PubMed Central identifier	PMC4724997
has PubMed identifier	26733065
schema:publication	mBio
resource representing a document's body	covid:131af5ab50551638e3ce9a8c615b9b4364f2f34e#body_text
is schema:about of	named entity 'MODEL' named entity 'EFFICACY' named entity 'THESE' named entity 'PROVIDING' named entity 'PATHOGENIC' named entity 'CLINICAL SETTING' named entity 'WILD-TYPE' named entity 'IS A' named entity 'TRANSMISSION' named entity 'PLAQUES' named entity 'SMALLER' named entity 'DEVELOPMENT' named entity 'OPEN READING FRAME' named entity 'CLONE' named entity 'IN VIVO' named entity 'FORMED' named entity 'REMOVED' named entity 'MOLECULAR' named entity 'SEEN' named entity 'TRANSMITTED' named entity 'ALPHACORONAVIRUS' named entity 'cell culture' named entity 'RFP' named entity 'efficiently' named entity 'replicated' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PLATFORM' named entity 'IDENTIFYING' named entity 'REGULATE' named entity 'PIGS' named entity 'KEY' named entity 'ROBUST' named entity 'CHARACTERIZATION' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PROTEIN ' named entity 'RECOMBINANT' named entity 'LIVE' named entity 'INFECTIOUS' named entity 'REGION' named entity 'EFFICIENCY' named entity 'UNITED STATES' named entity 'LENGTH' named entity 'CDNA CLONE' named entity 'STRAIN' named entity 'REVERSE' named entity 'RAPIDLY' named entity 'INDIRECT' named entity 'TYPE VIRUS' named entity 'INFECTED' named entity 'DESCRIBE' named entity 'STRAINS' named entity 'REVERSE GENETICS' named entity 'RED FLUORESCENT PROTEIN' named entity 'VIRULENCE' named entity 'CELL CULTURE' named entity 'RFP' named entity 'USING' named entity 'RESULTING IN' named entity 'GENETIC FACTORS' named entity 'LETHAL' named entity 'IN VITRO' named entity 'VIRULENT' named entity 'CONTACT' named entity 'STUDY' named entity 'PATHOGENIC' named entity 'STRAIN' named entity 'THERAPEUTICS' named entity 'PATHOGENESIS' named entity 'GENETICS' named entity 'REPLACED' named entity 'TRANSMISSION' named entity 'VIRUS'

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