About: Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23

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About: Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23
Creator	Yuen, Kwok-Yung Joseph, Sunitha Wernery, Renate Wernery, Ulrich Yip, Cyril Tsang, Chi-Ching Fan, Rachel Lau, Susanna Tsang, Alan Wong, Emily Woo, Patrick Lau, Candy
Source	Medline; PMC
abstract	Recently, we reported the discovery of a dromedary camel coronavirus UAE-HKU23 (DcCoV UAE-HKU23) from dromedaries in the Middle East. In this study, DcCoV UAE-HKU23 was successfully isolated in two of the 14 dromedary fecal samples using HRT-18G cells, with cytopathic effects observed five days after inoculation. Northern blot analysis revealed at least seven distinct RNA species, corresponding to predicted subgenomic mRNAs and confirming the core sequence of transcription regulatory sequence motifs as 5′-UCUAAAC-3′ as we predicted previously. Antibodies against DcCoV UAE-HKU23 were detected in 58 (98.3%) and 59 (100%) of the 59 dromedary sera by immunofluorescence and neutralization antibody tests, respectively. There was significant correlation between the antibody titers determined by immunofluorescence and neutralization assays (Pearson coefficient = 0.525, p < 0.0001). Immunization of mice using recombinant N proteins of DcCoV UAE-HKU23 and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, and heat-inactivated DcCoV UAE-HKU23 showed minimal cross-antigenicity between DcCoV UAE-HKU23 and MERS-CoV by Western blot and neutralization antibody assays. Codon usage and genetic distance analysis of RdRp, S and N genes showed that the 14 strains of DcCoV UAE-HKU23 formed a distinct cluster, separated from those of other closely related members of Betacoronavirus 1, including alpaca CoV, confirming that DcCoV UAE-HKU23 is a novel member of Betacoronavirus 1.
has issue date	2016-05-07(xsd:dateTime)
bibo:doi	10.3390/ijms17050691
bibo:pmid	27164099
has license	cc-by
sha1sum (hex)	13e902f62fb20b66fbbc69e9ec434243342d16cd
schema:url	https://doi.org/10.3390/ijms17050691
resource representing a document's title	Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23
has PubMed Central identifier	PMC4881517
has PubMed identifier	27164099
schema:publication	Int J Mol Sci
resource representing a document's body	covid:13e902f62fb20b66fbbc69e9ec434243342d16cd#body_text
is schema:about of	named entity 'CHARACTERIZATION' named entity 'MINIMAL' named entity 'SEROLOGICAL' named entity 'CROSS' named entity 'SPECIES' named entity 'RECENTLY' named entity 'SEPARATED FROM' named entity 'SEQUENCE MOTIFS' named entity 'FECAL' named entity 'RDRP' named entity 'INOCULATION' named entity 'DROMEDARY CAMEL CORONAVIRUS' named entity 'DETERMINED BY' named entity 'GENETIC DISTANCE' named entity 'MICE' named entity 'REGULATORY SEQUENCE' named entity 'CORE' named entity 'ANTIBODIES' named entity 'STUDY' named entity 'BETACORONAVIRUS 1' named entity 'DROMEDARY' named entity 'CORRELATION' named entity 'DISTINCT' named entity 'PROTEINS' named entity 'NORTHERN BLOT ANALYSIS' named entity 'UAE' named entity 'SEQUENCE' named entity 'CLUSTER' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'DROMEDARIES' named entity 'MIDDLE EAST' named entity 'ISOLATION' named entity 'MERS CORONAVIRUS' named entity 'FORMED' named entity 'GENES' named entity 'PREDICTED' named entity 'MRNAS' named entity 'WESTERN BLOT' named entity 'OBSERVED' named entity 'RELATED' named entity 'DISCOVERY' named entity 'REPORTED' named entity 'HRT-18G' named entity 'UAE' named entity 'ANTIBODY TESTS' named entity 'HEAT' named entity 'CYTOPATHIC EFFECTS' named entity 'MEMBERS' named entity 'ISOLATED' named entity 'PREVIOUSLY' named entity 'DROMEDARIES' named entity 'CROSS' named entity 'ANALYSIS' named entity 'NOVEL' named entity 'CONFIRMING' named entity 'ALPACA' named entity 'ANTIBODY TITERS' named entity 'SAMPLES' named entity 'CELLS' named entity 'ANTIGENICITY' named entity 'IMMUNOFLUORESCENCE' named entity '525' named entity 'DAYS' named entity 'STRAINS' named entity 'MIDDLE EAST' named entity 'MINIMAL' named entity 'TRANSCRIPTION' named entity 'COEFFICIENT' named entity 'REVEALED' named entity 'CODON USAGE'

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