About: Production of second-generation cloned cats by somatic cell nuclear transfer

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About: Production of second-generation cloned cats by somatic cell nuclear transfer Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Production of second-generation cloned cats by somatic cell nuclear transfer
Creator	Cho, S Lee, H Yin, X Yu, X Choi, E Kim, L Kong, I Shin, H
topic	covid:13eb0c246fa4c137333bc8cd3d87bb23eb8d5dc2#this
Source	Elsevier; Medline; PMC
abstract	Abstract We successfully produced second-generation cloned cats by somatic cell nuclear transfer (SCNT) using skin cells from a cloned cat. Skin cells from an odd-eyed, all-white male cat (G0 donor cat) were used to generate a cloned cat (G1 cloned cat). At 6 months of age, skin cells from the G1 cloned cat were used for SCNT to produce second-generation cloned cats. We compared the in vitro and in vivo development of SCNT embryos that were derived from the G0 donor and G1 cloned donor cat's skin fibroblasts. The nuclei from the G0 donor and G1 cloned donor cat's skin fibroblasts fused with enucleated oocytes with equal rates of fusion (60.7% vs. 58.8%, respectively) and cleavage (66.3% vs. 63.4%). The 2–4-cell SCNT embryos were then transferred into recipients. One of the five recipients of G0 donor derived NT embryos (20%) delivered one live male cloned kitten, whereas 4 of 15 recipients of the G1 cloned donor cat derived NT embryos (26%) delivered a total of seven male second-generation cloned kittens (four live kittens from one surrogate, plus two stillborn kittens, and one live kitten that died 2d after birth from three other surrogate mothers). The four second-generation cloned kittens from the same surrogate all had a white coat color; three of the four second-generation cloned kittens had two blue eyes, and one of the second-generation cloned kittens had an odd-eye color. Despite low cloning efficiency, cloned cats can be used as donor cats to produce second-generation cloned cats.
has issue date	2008-05-31(xsd:dateTime)
bibo:doi	10.1016/j.theriogenology.2008.01.017
bibo:pmid	18358524
has license	els-covid
sha1sum (hex)	13eb0c246fa4c137333bc8cd3d87bb23eb8d5dc2
schema:url	https://doi.org/10.1016/j.theriogenology.2008.01.017
resource representing a document's title	Production of second-generation cloned cats by somatic cell nuclear transfer
has PubMed Central identifier	PMC7127140
has PubMed identifier	18358524
schema:publication	Theriogenology
resource representing a document's body	covid:13eb0c246fa4c137333bc8cd3d87bb23eb8d5dc2#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/13eb0c246fa4c137333bc8cd3d87bb23eb8d5dc2
is schema:about of	named entity 'GENERATION' named entity 'oocytes' named entity 'surrogate' named entity 'development' named entity 'cloned' named entity 'live' named entity 'One' named entity 'cloned' named entity 'cloned' named entity 'Skin' named entity 'SURROGATE MOTHERS' named entity 'DONOR' named entity 'COAT COLOR' named entity 'ODD' named entity 'USING' named entity 'LOW' named entity 'STILLBORN' named entity 'FUSED WITH' named entity 'EQUAL' named entity 'COMPARED' named entity 'SEVEN' named entity 'CLEAVAGE' named entity 'MONTHS' named entity 'CLONING' named entity 'GENERATE' named entity 'USED' named entity 'GENERATION' named entity '4-CELL' named entity '2860' named entity 'IN VIVO' named entity 'NUCLEI' named entity 'ONE OF' named entity 'CLONED' named entity 'WHITE' named entity 'SOMATIC CELL NUCLEAR TRANSFER' named entity 'SKIN' named entity 'SOMATIC CELL NUCLEAR TRANSFER' named entity 'DERIVED' named entity 'DELIVERED' named entity 'EYE COLOR' covid:arg/13eb0c246fa4c137333bc8cd3d87bb23eb8d5dc2 named entity '27S' named entity 'CLONED' named entity 'PRODUCTION' named entity 'IN VITRO' named entity 'TO PRODUCE' named entity 'SCNT' named entity 'MALE' named entity 'OOCYTES' named entity 'TRANSFERRED' named entity 'CELLS' named entity 'BIRTH' named entity 'SECOND' named entity 'RATES' named entity 'FIBROBLASTS' named entity 'FUSION' named entity 'TOTAL' named entity 'AGE' named entity 'EMBRYOS' named entity 'CAT' named entity 'CATS' named entity 'DIED' named entity 'LIVE' named entity 'DEVELOPMENT' named entity 'SECOND' named entity 'CATS' named entity 'BLUE EYES' named entity 'SURROGATE' named entity 'EFFICIENCY' named entity 'died' named entity 'donor' named entity 'kittens'

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