About: An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection

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About: An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection
Creator	Stamminger, Thomas Münch, Jan Müller, Janis Ständker, Ludger Conzelmann, Carina Groß, Rüdiger Preising, Nico Sparrer, Konstantin Stenger, Steffen Schütz, Desiree Conzelmann, C Gilg, A Gilg, Andrea Groß, R Jahrsdörfer, Bernd Schrezenmeier, Hubert Schütz, D Stenger,
Source	Elsevier; Medline; PMC
abstract	SARS-CoV-2 is a novel pandemic coronavirus that caused a global health and economic crisis. The development of efficient drugs and vaccines against COVID-19 requires detailed knowledge about SARS-CoV-2 biology. Several techniques to detect SARS-CoV-2 infection have been established, mainly based on counting infected cells by staining plaques or foci, or by quantifying the viral genome by PCR. These methods are laborious, time-consuming and expensive and therefore not suitable for a high sample throughput or rapid diagnostics. We here report a novel enzyme-based immunodetection assay that directly quantifies the amount of de novo synthesized viral spike protein within fixed and permeabilized cells. This in-cell ELISA enables a rapid and quantitative detection of SARS-CoV-2 infection in microtiter format, regardless of the virus isolate or target cell culture. It follows the established method of performing ELISA assays and does not require expensive instrumentation. Utilization of the in-cell ELISA allows to e.g. determine TCID(50) of virus stocks, antiviral efficiencies (IC(50) values) of drugs or neutralizing activity of sera. Thus, the in-cell spike ELISA represents a promising alternative to study SARS-CoV-2 infection and inhibition and may facilitate future research.
has issue date	2020-07-29(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2020.104882
bibo:pmid	32738255
has license	no-cc
sha1sum (hex)	14775fc7745ca44ea6c00d0ed8dcb8a0e4d7ff63
schema:url	https://doi.org/10.1016/j.antiviral.2020.104882
resource representing a document's title	An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection
has PubMed Central identifier	PMC7388004
has PubMed identifier	32738255
schema:publication	Antiviral Res
resource representing a document's body	covid:14775fc7745ca44ea6c00d0ed8dcb8a0e4d7ff63#body_text
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